Nanosecond-time-resolved infrared spectroscopic study of fast relaxation kinetics of protein folding by means of laser-induced temperature-jump

doi:10.1088/1009-1963/14/12/019

Abstract

Abstract Elucidating the initial kinetics of folding pathways is critical to the understanding of the protein folding mechanism. Transient infrared spectroscopy has proved a powerful tool to probe the folding kinetics. Herein we report the construction of a nanosecond laser-induced temperature-jump ($T$-jump) technique coupled to a nanosecond time-resolved transient mid-infrared (mid-IR) spectrometer system capable of investigating the protein folding kinetics with a temporal resolution of 50 ns after deconvolution of the instrumental response function. The mid-IR source is a liquid N$_{2}$ cooled CO laser covering a spectral range of 5.0$\mu$m (2000 cm$^{-1}) \sim $ 6.5$\mu$m (1540 cm$^{-1})$. The heating pulse was generated by a high pressure H$_{2}$ Raman shifter at wavelength of 1.9$\mu$m. The maximum temperature-jump could reach as high as 26$\pm $1$^{\circ}$C. The fast folding/unfolding dynamics of cytochrome $C$ was investigated by the constructed system, providing an example.

Keywords: protein folding sub-microsecond laser-induced temperature-jump transient infrared spectrum dynamics cytochrome $C$

Received: 06 July 2005
Revised: 08 September 2005
Accepted manuscript online:

PACS:	87.15.M-	(Spectra of biomolecules)
	87.15.Cc	(Folding: thermodynamics, statistical mechanics, models, and pathways)
	87.15.H-	(Dynamics of biomolecules)
	87.14.E-	(Proteins)

Fund: Project supported by the National Natural Science Foundation of China (Grant No 20373088) and Program for innovation group (Grant No 60321002).

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Zhang Qing-Li (张庆利), Wang Li (王莉), Weng Yu-Xiang (翁羽翔), Qiu Xiang-Gang (邱祥冈), Wang Wei-Chi (王渭池), Yan Ji-Xiang (阎吉祥) Nanosecond-time-resolved infrared spectroscopic study of fast relaxation kinetics of protein folding by means of laser-induced temperature-jump 2005 Chinese Physics 14 2484

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Nanosecond-time-resolved infrared spectroscopic study of fast relaxation kinetics of protein folding by means of laser-induced temperature-jump

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