|
|||||
|
|
| α-萘基缩水甘油醚对映体的毛细管区带电泳手性拆分 | |
| 引用本文： | 肖溶,张丹,杜培刚,罗月影,赵欣,刘燕,张乾.α-萘基缩水甘油醚对映体的毛细管区带电泳手性拆分[J].分析化学,2009,37(11). |
| 作者姓名： | 肖溶 张丹 杜培刚 罗月影 赵欣 刘燕 张乾 |
| 作者单位： | 1. 四川大学华西药学院,成都,610041;贵州省首钢水钢总医院临床药学实验室,六盘水,553028 2. 四川大学华西药学院,成都,610041 3. 贵州省首钢水钢总医院临床药学实验室,六盘水,553028 |
| 基金项目： | 贵州省卫生厅科学技术基金项目，贵州省六盘水市科技攻关计划项目 |
| 摘 要： | 建立了毛细管区带电泳手性拆分α-萘基缩水甘油醚对映体的方法.考察了不同手性拆分试剂对手性选择性的影响,实验结果表明,20 mmol/L H3PO4-三乙醇胺(pH 2.5)、2%(w/V)HS-β-CD、毛细管温度20 ℃、运行电压-18 kV为最佳分离条件,在该分离条件下α-萘基缩水甘油醚对映体实现基线分离.方法简便、准确,可用于α-萘基缩水甘油醚的手性拆分和对映体过量值(ee,%)测定. |
| 关 键 词： | α-萘氧缩水甘油醚 手性拆分 毛细管电泳 环糊精 |
Chiral Separation of Naphthylglycidic Ether Using Capillary Zone Electrophoresis |
|
| XIAO Rong,ZHANG Dan,DU Pei-Gang,LUO Yue-Ying,ZHAO Xin,LIU Yan,ZHANG Qian.Chiral Separation of Naphthylglycidic Ether Using Capillary Zone Electrophoresis[J].Chinese Journal of Analytical Chemistry,2009,37(11). | |
| Authors: | XIAO Rong ZHANG Dan DU Pei-Gang LUO Yue-Ying ZHAO Xin LIU Yan ZHANG Qian |
| Institution: | XIAO Rong1,2,ZHANG Dan* 1,DU Pei-Gang2,LUO Yue-Ying2,ZHAO Xin2,LIU Yan2,ZHANG Qian2 1 ( West China Pharmacy School of Sichuan University,Chengdu 610041) 2 ( Clinic Pharmacy Research Laboratory,Guizhou Shougang Shuigang Genaral Hospital,Liupanshui 553028) |
| Abstract: | A capillary electrophoretic method was developed for the separation of enantiomers of naphthylgly-cidic ether(NGE). Several cyclodextrins(CDs) were applied as the chiral selectors and it was found that the ionic modified highly sulfated cyclodextrin(HS-β-CD) could give satisfactory enantioselectivity. In addition,the effects of the pH value of the buffer system,the concentration of the HS-β-CD,capillary temperature and the running voltage on the chiral separation were investigated systematically. The result showed that under the optimized conditions of pH 2. 5,20 mmol/L H_3PO_4-triethanolamine buffer containing 2% highly sulfated cyclodextrin ( HS-β-CD) at capillary temperature 20℃ in the reversed polarity voltage of - 18 kV,the enantiomers of naphthylglycidic ether were baseline separated. This method is simple,precise and can be applied to the chiral separation of naphthylglycidic ether enantiomers and determination of enantiomers excess(ee,% ). |
| Keywords: | Naphthylglycidic ether chiral separation capillary electrophoresis cyclodextrins |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |