|
|||||
|
|
Poly(ethylene oxide) macromonomer based hydrogels as a template for the culture of hepatocytes |
|
| Authors: | Eliane Alexandre Jacques Cinqualbre Daniel Jaeck Lysiane Richert François Isel Pierre J Lutz |
| Institution: | 1. Fondation Transplantation, Laboratoire de Chirurgie Experimentale, 5, Avenue Molière, F-67200 Strasbourg Cedex, France;2. Fondation Transplantation, Laboratoire de Chirurgie Experimentale, 5, Avenue Molière, F-67200 Strasbourg Cedex, France
Faculté de Médecine et de Pharmacie, Laboratoire de Biologie Cellulaire, 4, Place St Jacques, F-25030 Besançon Cedex, France;3. Institut C. Sadron, CNRS, UPR22 F-67083 Strasbourg Cedex, France |
| Abstract: | Macromonomer based poly(ethylene oxide) (PEO) hydrogels were tested with respect to their ability to serve as a template for the survival and the growth of hepatocytes. Two systems were considered : either the surface of preexisting hydrogels, with controlled structural parameters, were seeded with isolated rat hepatocytes or the hepatocytes were dispersed in physiological medium containing the macromonomer/initiator and heated to 37°C. In the first case, cells were examined at given times after spreading over two days. The results were compared to those observed for the dispersion of fibroblasts onto a surface of the same type of hydrogels. The effects of the structure of the hydrogels and its chemical nature on the extent of hepatocyte attachment (or encapsulation) and the morphology were investigated. |
| Keywords: | biomaterials crosslinking hepatocyte encapsulation hydrogels polyethylene oxide |