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排序方式: 共有63条查询结果,搜索用时 15 毫秒
1.
Allison Leyton Michael Araya Fadia Tala Liset Flores María Elena Lienqueo Carolina Shene 《Molecules (Basel, Switzerland)》2021,26(8)
Seaweed processing generates liquid fraction residual that could be used as a low-cost nutrient source for microbial production of metabolites. The Rhodotorula strain is able to produce antimicrobial compounds known as sophorolipids. Our aim was to evaluate sophorolipid production, with antibacterial activity, by marine Rhodotorula rubra using liquid fraction residual (LFR) from the brown seaweed Macrocystis pyrifera as the nutrient source. LFR having a composition of 32% w/w carbohydrate, 1% w/w lipids, 15% w/w protein and 52% w/w ash. The best culture condition for sophorolipid production was LFR 40% v/v, without yeast extract, artificial seawater 80% v/v at 15 °C by 3 growth days, with the antibacterial activity of 24.4 ± 3.1 % on Escherichia coli and 21.1 ± 3.8 % on Staphylococcus aureus. It was possible to identify mono-acetylated acidic and methyl ester acidic sophorolipid. These compounds possess potential as pathogen controllers for application in the food industry. 相似文献
2.
The effects of ultraviolet-C radiation (UV-C, 11.8?W/m2), single-cycle and multiple-cycle high hydrostatic pressure (HHP at 200, 400 or 600?MPa) on microbial load and physicochemical quality of raw milk were evaluated. Reductions of aerobic plate count (APC) and coliform count (CC) by HHP were more than 99.9% and 98.7%, respectively. Inactivation efficiency of microorganisms increased with pressure level. At the same pressure level, two-cycle treatments caused lower APC, but did not show CC differences compared with single-cycle treatments. Reductions of APC and CC by UV-C were somewhere between 200?MPa and 400/600?MPa. Both HHP and UV-C significantly decreased lightness and increased pH, but did not change soluble solids content and thiobarbituric acid-reactive substances’ values. Two 2.5?min cycles of HHP at 600?MPa caused minimum APC and CC, and maximum conductivity. Compared with HHP, UV-C markedly increased protein oxidation and reduced darkening. 相似文献
3.
Takeshi Yamada Yuki MizutaniYoshihide Umebayashi Naoko InnoMaiko Kawashima Takashi KikuchiReiko Tanaka 《Tetrahedron letters》2014
Tandyukisin (1), a novel decalin derivative with an enolic β-ketoaldehyde, has been isolated from a strain of Trichoderma harzianum OUPS-111D-4 originally derived from the marine sponge Halichondria okadai, and its structure has been elucidated on the basis of spectroscopic analyses using 1D and 2D NMR techniques. In addition, the absolute configuration for 1 was established by the application of CD spectrum to the tribenzoate derivative. This compounds exhibited moderate cytotoxicity against human cancer cell lines. 相似文献
4.
Quantitative Detection for Porphyromonas gingivalis in Tooth Pocket and Saliva by Portable Electrochemical DNA Sensor Linked with PCR 下载免费PDF全文
Keiichiro Yamanaka Shinichi Sekine Takahiro Uenoyama Masahiro Wada Tomohiko Ikeuchi Masato Saito Yoshinori Yamaguchi Eiichi Tamiya 《Electroanalysis》2014,26(12):2686-2692
Here, a quantitative electrochemical analysis of periodontal bacteria in gingival crevicular fluid (GCF) and saliva by direct polymerase chain reaction (PCR) is presented. The electrochemical measurement was performed by mixing with PCR products and electrochemical indicator (bisbenzimidazole trihydrochloride). The peak current of indicator is reduced due to slower diffusion when the dye intercalates into the amplified DNA, and the degree of reduction in the peak current is correlates with the quantity of amplified DNA. Therefore, a quantitative analysis is possible by using our electrochemical method at the end point of PCR. In the GCF testing, The number of Porphyromonas gingivalis (Pg) detected by our electrochemical method at the end point of PCR were almost same compared with that were calculated by the conventional method of quantitative real? time PCR. In the saliva testing, the relationship between number of Pg in saliva and average pocket depth, and age‐dependence were also clearly observed. Since the saliva sample is obtained in a non‐invasive manner, this method is useful for the primary screening of periodontal disease. Moreover, our detection method is simple and uses a hand‐held potentiostat making it suitable for development of an on‐site periodontal diagnosis system. 相似文献
5.
砷锑污染在全球领域广泛存在,与常规的铁氧化物相比,微生物铁氧化生成的含Fe(Ⅲ)矿物对水中砷/锑(As/Sb)具有更强的吸附能力,并因其高效、实用和环境友好而具有广阔的应用前景,但微生物铁还原也可能导致被吸附的As/Sb再次释放。本文综述了微生物铁氧化还原作用对As/Sb去除影响的研究进展,归纳了铁矿物“合成-溶解-转化”的微生物循环过程以及该循环伴随的水中As/Sb固定、溶解与转化机理,整合了微生物合成Fe(Ⅲ)矿物的矿物学性质、对As/Sb固定的热动力学规律和络合机制,总结了微生物合成Fe(Ⅲ)矿物对As/Sb去除的影响因素,基于该研究的现存问题展望了利用微生物铁氧化还原作用去除As/Sb的发展方向。 相似文献
6.
超高压(High Hydrostatic Pressure,HHP)作为一种非热杀菌技术,在食品工业中有着很广泛的应用,与传统的热加工处理相比,在保持食品品质、杀菌、钝酶等方面都有其明显的优势。通过对国内外相关文献的分析,对HHP处理后处于亚致死状态食品微生物的细胞膜、遗传与结构物质、机体代谢等方面,以及大部分食品微生物在应对外界的多种刺激时而进行的普遍性调控机制的研究进行了总结,探讨了经过HHP处理后处于亚致死状态的食品微生物在逆境下存活的应激反应。 相似文献
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9.
Recent analytical innovations for nucleic acid detection have revolutionized the biological sciences. Single nucleic acid sequence detection methods have been expanded to incorporate multiplexed detection strategies. A variety of nucleic acid detection formats are now available that can address high throughput genomic interrogation. Many of these parallel detection platforms or arrays, employ fluorescence as the signaling method. Fluorescence-based assays offer many advantages, including increased sensitivity, safety and multiplexing capabilities, as well as the ability to measure multiple fluorescence properties. Multiplexed microarray platforms provide parallel detection capabilities capable of measuring thousands of simultaneous responses. This review will discuss both single target detection and microarray applications with a focus on gene expression and pathogenic microorganism (PM) detection. 相似文献
10.
V. S. Gorelik A. A. Esakov A. N. Morozov P. P. Sverbil’ S. D. Tyves 《Journal of Russian Laser Research》2008,29(1):71-89
We developed a method for detecting traces of foreign components in water and analyzing the microstructure of water. The method
is based on studies of the characteristics of secondary emission arising in aqueous media under excitation by radiation of
ultraviolet lasers and light emitting diodes. A new type of cuvette (capillary cuvettes) for studying the secondary emission
in molecular media was elaborated. The cuvettes enable the study of aqueous solutions at small concentrations of the components;
they enable one to recognize the type of molecular compound present in aqueous media, in view of the photoluminescence spectra. 相似文献