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Květa Kalíková Petr Kozlík Martin Gilar Eva Tesařová 《Journal of separation science》2013,36(15):2421-2429
Hydrophilic interaction liquid chromatography is a separation technique suitable for the separation of moderately and highly polar compounds. Various stationary phases (SPs) for hydrophilic interaction liquid chromatography are commercially available. While the SPs based on the same type of ligand are available from different providers, they can display a distinct retention characteristics and separation selectivity. The current work is focused on characterization and comparison of the separation systems of two amide‐based HPLC columns from two producers, i.e. XBridge Amide column and TSK gel Amide‐80 column. Several characterization procedures (tests) were used to investigate the differences between these columns. The chromatographic behavior of selected analytes indicates that multimodal interactions are responsible for retention and separation on these columns. Multiple testing approaches were used in order to reveal subtle differences between the SPs. Both amide‐based columns showed certain differences in retention, selectivity, and plate counts. Based on the tests used in this study, we conclude that the investigated columns provide a different degree of H‐bonding interactions. 相似文献
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一种全场三维共焦检测的新方法 总被引:11,自引:1,他引:10
提出了一种利用微光学阵更合成器件实现全场三维面形并行检测、记录的共焦方法。此方法的关键是,在共焦系统中引入一一微光学耻列合成器件来产生一点光源阵列,实现了对同一一剖面同时并行检测,并首次采用了CCD面阵像元取代上孔光阑直接截取三维信息光强。本文讨论了该方法的三信息检测原理,响应关系,给出了初步的实验结果和三维重构图。 相似文献
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Takafumi Matsuda Kenichi Yamashita Hideaki Maeda Masahiko Hashimoto Kazuhiko Tsukagoshi 《Journal of separation science》2013,36(5):965-970
A capillary chromatography system has been developed using a ternary mixed‐solvents solution, i.e. water–hydrophilic/hydrophobic organic solvent mixture as a carrier solution. Here, we tried to carry out the chromatographic system on a microchip incorporating the open‐tubular microchannels. A model analyte solution of isoluminol isothiocyanate (ILITC) and ILITC‐labeled biomolecule was injected to the double T‐junction part on the microchip. The analyte solution was delivered in the separation microchannel (40 μm deep, 100 μm wide, and 22 cm long) with the ternary water–ACN–ethyl acetate mixture carrier solution (3:8:4 volume ratio, the organic solvent rich or 15:3:2 volume ratio, the water‐rich). The analyte, free‐ILITC and labeled BSA mixture, was separated through the microchannel, where the carrier solvents were radially distributed in the separation channel generating inner and outer phases. The outer phase acts as a pseudo‐stationary phase under laminar flow conditions in the system. The ILITC and the labeled BSA were eluted and detected with chemiluminescence reaction. 相似文献
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镍,钴与zincon的络合吸附波研究:铜精矿中微量镍,钴的同时测定 总被引:1,自引:0,他引:1
有关利用络合物的极谱催化波连续测定镍,钴的报道较少。在研究铜-Zincon的极谱行为时,我们发现NH_4Cl-Na_2SO_3(pH=6.5~7.5)底液中当Zincon浓度>4×10~(-5)·mol/L时,单扫描示波极谱仪上有两个峰,其峰电位分别为-0.42V和-0.56v(vs. 相似文献