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研究了胸腺五肽(TP5)在NH-1树脂上的离子交换过程.结果表明,在pH 2.O的条件下,其等温吸附过程符合Langmuir等温吸附方程,最大吸附量为221.07mg/g wet resin;静态吸附动力学的研究结果显示,NH-1树脂对TP5的吸附过程主要受颗粒扩散控制影响,TP5的颗粒内扩散系数为4.50×10-9cm2/s;对比Nernst-Planck与Fick动力学模型,可用Fick动力学模型来描述TP5在NH-1树脂内的颗粒扩散行为,其有效扩散系数为5.58×10-9cm2/s;采用固定床吸附数学模型,对TP5离子交换柱行为进行模拟,该数学模型考虑了颗粒扩散、轴向返混、非线性平衡以及液膜扩散.结果显示,模拟数据与实验结果具有较好的符合. 相似文献
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Z-Asp-Val-Tyr-NH2,a precursor tripeptide of thymopentin(TP-5),was successfully synthesized by a combination of chemical and enzyme methods.Firstly,the precursor dipeptide Val-Tyr-NH2 was synthesized by a novel chemical method in four steps including the preparation of NCA-Val,the esterification of L-tyrosine and the ammonolysis of L-tyrosine ethyl ester,and the formation of Val-Tyr-NH2 from NCA-Val and L-Tyr-NH2.Secondly,a thermolysin,thermoase PC 10F was used to catalyze the formation of Z-Asp-Val-Tyr-NH2 in an aqueous/organic solvent diphase system with Z-Asp-OH and Val-Ty-NH2 as the substrates under thermodynamic control.The optimum conditions were pH=6.5,40 ℃,thermoase 100 mg,in 2-(N-morpholino) ethanesulfonic acid MES/NaOH(containing 5 mmol/L CaCl2) of n-butanol system(15/85,volume ratio) for 20 h in a maximum yield of 27.02%. 相似文献
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Chaoli Wang Xiaoling Hu Ping Guan Liwei Qian Danfeng Wu Ji Li 《International Journal of Polymer Analysis and Characterization》2014,19(1):70-82
Ionic liquids are also called “designer solvents.” In this article, we calculated the interaction energy of four ionic liquids with a template by molecular dynamics simulation. A simple approach was used to prepare biomacromolecule molecularly imprinted polymers for adsorbent and separation thymopentin (Tp5). In order to overcome intrinsic and increase structural selectivity, surface-initiated ATRP and ionic liquids (ILs) as functional monomer were used to prepare Fe3O4 molecularly imprinted polymers. Selective adsorption was applied to investigate the interactions between the polymers and lysine, phenylalanine, glutathione (GSH), and hemoglobin. The results demonstrated that Fe3O4 MIPs with 1-vinyl-3-ethyl acetate imidazolium chloride as functional monomer demonstrated high isolation and recognition of performance to the Tp5. 相似文献
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Xin Li Lei Zhang Yonghui Chang Shubao Shen Hanjie Ying Pingkai Ouyang 《Chromatographia》2007,66(3-4):231-235
The ion-exchange kinetics have been determined for adsorption of thymopentin on a gel-type sulfonated styrene–divinylbenzene
resin converted to the ammonium form. Batch equilibrium and kinetic experiments were performed in chloride ion solutions of
different concentration. Equilibrium data revealed isotherms were a good fit to the constant separation factor isotherm. Because
of the high capacity and low cost of the resin its use for uptake of thymopentin was economically feasible. Kinetic data were
compared with the predictions from the Nernst–Planck and Fick models. The intraparticle and effective diffusivity of thymopentin
were obtained from these models. 相似文献
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Determination of thymopentin in beagle dog blood by liquid chromatography with tandem mass spectrometry and its application to a preclinical pharmacokinetic study 下载免费PDF全文
Meiyun Shi Yan Yang Xiaotong Zhou Lanlan Cai Chunxue Fang Can Wang Heping Sun Yantong Sun Yin Gao Jingkai Gu J. Paul Fawcett 《Journal of separation science》2015,38(8):1351-1357
The pentapeptide thymopentin (Arg‐Lys‐Asp‐Val‐Tyr, RKDVY) corresponds to amino acids 32–36 of the 49 amino acid immunomodulatory polypeptide, thymopoietin, whose biological activity is partially reproduced. Thymopentin is widely used in the clinic and represents a promising target for drug design but bioanalytical and pharmacokinetic data are limited due to its enzymatic instability. This paper reports a rapid and sensitive method based on liquid chromatography with tandem mass spectrometry for the determination of thymopentin in beagle dog blood. To inactivate peptidases and stabilize thymopentin, acetonitrile was added to blood samples immediately after collection followed by addition of stable isotope‐labeled thymopentin as internal standard and washing with dichloromethane. Chromatography was carried out on an Ascentis Express Peptide ES‐C18 column using gradient elution with methanol and aqueous 0.1% formic acid at a flow rate of 0.6 mL/min. Positive electrospray ionization mass spectrometry with selected reaction monitoring achieved linearity in the range of 1.5–800 ng/mL with good accuracy/precision and minimal matrix effects. The method was successfully applied to a pharmacokinetic study in beagle dogs after intravenous administration of 0.2 mg/kg thymopentin. 相似文献
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LIANG Yong-tao MI Hao-yu WANG Ming-zhe YU Li-ya LIU Zhong-hui WANG En-si . College of Life Science Jilin University Changchun P. R. China . Changchun University of Chinese Medicine Changchun . College of Basic Medical Sciences . College of Phamacy 《高等学校化学研究》2009,25(2)
Thymopentin derivative(septapeptide) was prepared by means of the solid-phase peptide synthesis method in which stepwise and segment condensations were involved.The structures of the key intermediate and target molecule were confirmed by ESI-MS,elementary analysis,IR,1H NMR and 13C NMR spectrometries in our experiments.In this study,the authors found that both thymopentin derivetive(septapeptide) and thymopentin could promote T cell proliferation and increase of E-rose fomation cells. 相似文献
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