Electrochemiluminescence Enhanced by the Synergetic Effect of Porphyrin and Multi-walled Carbon Nanotubes for Uric Acid Detection

The design and exploration of efficient, stable and environmentally compatible organic emitters for an electrochemiluminescence (ECL) sensor is a promising topic. Herein, a novel environmentally-friendly luminophore, ZnBCBTP@MWCNTs, were fabricated via self-assembly of porphyrin molecules (ZnBCBTP) onto multi-walled carbon nanotubes (MWCNTs). The resulting luminophore ZnBCBTP@MWCNTs displayed not only the highly ECL property and but also the good accelerated electron mobility. Then, a label-free ECL biosensor based ZnBCBTP@MWCNTs was constructed for the ultrasensitive detection of uric acid. Excitingly, this proposed ECL biosensor performed a good linear relationship in the range of 0–300 μM with a low detection limit of 1.4 μM, thus offering another reliable and feasible sensing platform for clinical bioanalysis with good selectivity, stability, and repeatability.     

An In Situ Investigation of the Protein Corona Formation Kinetics of Single Nanomedicine Carriers by Self-Regulated Electrochemiluminescence Microscopy

Protein coronas are present extensively at the bio-nano interface due to the natural adsorption of proteins onto nanomaterials in biological fluids. Aside from the robust property of nanoparticles, the dynamics of the protein corona shell largely define their chemical identity by altering interface properties. However, the soft coronas are normally complex and rapidly changing. To real-time monitor the entire formation, we report here a self-regulated electrochemiluminescence (ECL) microscopy based on the interaction of the Ru(bpy)₃³⁺ with the nanoparticle surface. Thus, the heterogeneity of the protein corona is in situ observed in single nanoparticle “cores” before and after loading drugs in nanomedicine carriers. The label-free, optical stable and dynamic ECL microscopy minimize misinterpretations caused by the variation of nanoparticle size and polydispersity. Accordingly, the synergetic actions of proteins and nanoparticles properties are uncovered by chemically engineered protein corona. After comparing the protein corona formation kinetics in different complex systems and different nanomedicine carriers, the universality and accuracy of this technique were well demonstrated via the protein corona formation kinetics curves regulated by competitive adsorption of Ru(bpy)₃³⁺ and multiple proteins on surface of various carriers. The work is of great significance for studying bio-nano interface in drug delivery and targeted cancer treatment.     

Studies on the electrochemiluminescent behavior of luminol on indium tin oxide (ITO) glass

The electrochemiluminescence (ECL) of luminol on indium tin oxide (ITO) glass was high even under a low potential around 0.4-0.5 V, which was quite different from other electrodes such as platinum. ITO nanoparticles were synthesized and used in the research on ITO glass in the ECL process. A static interaction between ITO and luminol is confirmed from UV-vis and fluorescence spectra. Then the ECL enhancement can be supposed to originate from the adsorption of luminol on ITO, which facilitated luminol’s oxidization to the excited state, giving out ECL. On the other hand, ITO can catalyze the generation of reactive oxygen species (ROSs), similar to some other nanomaterials, which also favored the ECL enhancement of luminol.     

Fundamentals and bioanalytical applications of functional quantum dots as electrogenerated emitters of chemiluminescence

Since the electrochemiluminescence (ECL) of quantum dots (QDs) of silicon was reported by Science in 2002, lots of QDs (e.g., II-VI, III-V and IV-VI) with different sizes and shapes have been used as ECL emitters for bioanalysis. Especially, QDs functionalized with multitudinous biomolecules offer excellent ECL signal-transduction platforms for designing a new generation of biosensing devices.In this article, we focus on recent advances in the ECL principles of functional QDs, and their bioanalytical applications in DNA analysis, immunoassay, cytosensing and detection of other biological molecules.     

Determination of nicotine and its metabolite cotinine in urine and cigarette samples by capillary electrophoresis coupled with electrochemiluminescence

In this paper, CE coupled with electrochemiluminesence (ECL) detection using a 76‐μm Pt disk as working electrode was developed for nicotine (NIC) determination. The major metabolite of NIC is cotinine (COT), which has a similar tertiary amine structure to NIC. However, there is a carbonyl group attached in the structure of COT, which leads to the great decrease in ECL response. In order to improve the ECL response of COT, NaBH₄ was used for carbonyl reduction. After reduction, NIC and COT were separated and detected by CE‐ECL. ECL response plotted with NIC concentration was linear between 5.0×10^?7 and 5.0×10^?5 mol/L (81–8100 μg/L), with LOD of 5.0×10^?8 mol/L (8.1 μg/L). The developed CE‐ECL method was applied for NIC determination in urine and cigarette samples.     

电泳沉积制备ZnO-C60和ZnO-MWCNT合涂层电极及其光电性质

在氧化铟锡( ITO)导电玻璃表面电泳沉积制备ZnO-C60和ZnO-MWCNT复合涂层电极,经后续热处理增强其结合强度,通过SEM观察2种电极复合涂层的表面形貌,并使用CHI 705电化学分析仪和PMI-E电致化学发光系统测定其光电性质.结果表明,ZnO-C60复合涂层电极具有较高的稳定性,在可见光辐照下,该电极显示...     

基于碳纳米管修饰电极的胆碱电化学发光生物传感器研制

在碳纳米管(CNTs)和K3Fe(CN)6修饰的铂电极上吸附固定胆碱氧化酶,以鲁米诺为发光试剂,研制了胆碱电化学发光(ECL)生物传感器.CNTs可有效提高电极表面的电荷传输能力、提高电极表面的生物相容性和对酶分子的固载能力;K3Fe(CN)6对酶活性具有激活作用,同时对H2O2增敏的鲁米诺ECL有增强作用,均有利于提...     

铱配合物的电致化学发光性能研究

结构相关的铱配合物[Ir(ppy)2L1](PF6)和[Ir(ppy)2L2](PF6)(ppy=2-苯基吡啶,LI=4-(2,2'-联吡啶-3-乙炔基)-N-(吡啶-2-亚甲基)-N-(噻吩-3-亚甲基)苯胺,L2=4-(2,2'-联吡-3-乙炔基)-N-二(吡啶-2-亚甲基)-苯胺)都具有优良的电致发光(ECL)性...     

CdS修饰玻碳电极电化学发光法检测三聚氰胺

本文将CdS纳米棒修饰到玻碳电极上制作了CdS修饰玻碳电极(CdS/GCE)并研究了其在K2S2O8溶液中的电化学发光(ECL)性能.基于三聚氰胺能淬灭CdSK2s2O8电化学发光的性质建立了一种检测三聚氰胺的方法.在最优条件下,体系的ECL强度与三聚氰胺浓度在1.0×10-9～1.0×10-7 mol/L范围内与EC...