保护私有信息的一般线性方程组计算协议

作为科学计算的一个重要问题,保护私有信息的线性方程组的求解在金融、机械及通信等领域有着广泛的应用.在不经意传输的意义下,利用有限域上计算Moore-Penrose伪逆矩阵的概率算法,设计新的安全协议,解决了隐私保护的一般线性方程组在有限域上的安全两方计算问题,并利用模拟范例证明该协议在半诚实模型下是安全的.     

Development of an enzyme-linked immunosorbent assay (ELISA)-like fluorescence assay to investigate the interactions of glycosaminoglycans to cells

Sulfated glycosaminoglycans were labeled with biotin to study their interaction with cells in culture. Thus, heparin, heparan sulfate, chondroitin 4-sulfate, chondroitin 6-sulfate and dermatan sulfate were labeled using biotin-hydrazide, under different conditions. The structural characteristics of the biotinylated products were determined by chemical (molar ratios of hexosamine, uronic acid, sulfate and biotin) and enzymatic methods (susceptibility to degradation by chondroitinases and heparitinases). The binding of biotinylated glycosaminoglycans was investigated both in endothelial and smooth muscle cells in culture, using a novel time resolved fluorometric method based on interaction of europium-labeled streptavidin with the biotin covalently linked to the compounds. The interactions of glycosaminoglycans were saturable and number of binding sites could be obtained for each individual compound. The apparent dissociation constant varied among the different glycosaminoglycans and between the two cell lines. The interactions of the biotinylated glycosaminoglycans with the cells were also evaluated using confocal microscopy. We propose a convenient and reliable method for the preparation of biotinylated glycosaminoglycans, as well as a sensitive non-competitive fluorescence-based assay for studies of the interactions and binding of these compounds to cells in culture.     

Treatment of SMC Composite Waste for Recycling as Reinforcing Fillers in Thermoplastics

Needs for recycling thermoset composite materials are considerably increasing. Unfortunately, such materials are particularly difficult to recycle due to their crosslinked nature. In this paper, we focus on the case of Sheet Moulding Compounds (SMC), which consist of an unsaturated polyester filled with glass fibres and calcium carbonate. We consider the possibilities of mechanical treatments allowing to obtain ground fractions that could be used as fillers for the reinforcement of thermoplastics. Two processes are described and compared with an existing industrial one. Finally, optimised fractions of SMC waste are introduced into polypropylene, leading to promising results in term of mechanical properties.     

不确定非线性光电伺服系统滑模自适应控制

针对同时存在参数不确定性和结构不确定性的非线性光电伺服系统,运用自适应原理对系统的参数进行在线估计,同时提出一种改进的指数趋近律,并结合滑模控制（变结构控制）策略设计系统的滑模自适应位置控制器（APR）。以等效正弦1.2sin(0.93t)作为仿真输入,在0.45s后跟踪误差小于60μrad,表明该控制方法对此类不确定非线性系统的控制效果良好。     

MECHANICAL PROPERTIES OF SMC WHISKER REINFORCED HIGH DENSITY POLYETHYLENE COMPOSITES

A new type of SiO_2-MgO-CaO (SMC) whisker was used to modify high density polyethylene (HDPE).The melting behavior and crystallinity were investigated by differential scanning calorimetry (DSC).The dispersion of whiskers and interfacial adhesion in the prepared HDPE/SMC whisker composites were investigated by scanning electron microscopy (SEM).The mechanical properties were evaluated by mechanical tests and dynamic mechanical analysis (DMA).DSC data indicated that the melting temperature and the crystall...     

Dynamic modelling and input-energy comparison for the elevator system

The elevator system driven by a permanent magnet synchronous motor (PMSM) is studied in this paper. The mathematical model of the elevator system includes the electrical and mechanical equations, and the dimensionless forms are derived for the purpose of practicable upward and downward movement. In this paper, the trapezoidal, cycloidal, five-degree (5-D) and seven-degree (7-D) polynomial and industry trajectories are designed and compared numerically in various motion and the absolute input energies. From numerical simulations, it is found that the trapezoidal trajectory consumes the minimum energy; the 7-D polynomial trajectory consumes the maximum one. The less end-point constraints are required, the less energy is consumed. Finally, the proposed sliding mode controller (SMC) is employed to demonstrate the robustness and well tracking control performance numerically.     

Genetic characterization of Escherichia coli RecN protein as a member of SMC family of proteins

The proteins of SMC family are characterized by having Walker A and B sites. The Escherichia coli RecN protein is a prokaryotic member of SMC family that involved in the induced excision of Tn10 and the repair of the DNA double strand breaks. In this work, the Walker A nucleotide binding site of the E. coli RecN protein was mutated by changing the highly conserved lysine residue 35 to the aspartic acid (D), designated as recN_K35D. Reverse genetics was utilized to delete the entire recN gene (ΔrecN108) or introduce the recN_K35D gene into the E. coli chromosomal DNA. The recN_K35D cells showed decrease in the frequency of excision of Tn10 from gal76::Tn10 after treatment with mitomycin C compared to recN⁺ cells. The ΔrecN108 cells showed an un-induced increase in the frequency of Tn10 excision from gal76::Tn10 in rec⁺ background while, recBC sbcBC ΔrecN108 cells are completely deficient in Tn10 excision. The recombination proficiency is reduced in cells carrying recBC sbcBC cells in addition recN_K35D mutation. We observed that the Walker A nucleotide binding site is important for the RecN protein. Strains that deleted recN gene are recombination deficient and more sensitive to mitomycin C than strains carrying recN_K35D.     

去除土壤水分对高光谱估算土壤有机质含量的影响

土壤高光谱技术具有方便快捷、无破坏、成本低等优点,已被广泛应用于估算土壤有机质含量(SOMC)。然而,野外测量的土壤高光谱数据因受外部环境因素(土壤湿度、温度、表面粗糙度等)干扰,导致SOMC估算模型适用性有待提升。土壤含水率(SMC)是影响野外测量高光谱的最主要的障碍因素之一,它的变化严重影响可见-近红外(Vis-NIR)光谱反射率的观测结果。因此,消除SMC对高光谱数据的干扰是提高土壤高光谱估算SOMC模型预测精度的关键环节。以江汉平原潜江市潮土样本为研究对象,在室内人工加湿土样,分别获取6个SMC水平的土壤高光谱数据,采用标准正态变换(SNV)对光谱数据进行预处理,基于外部参数正交化法(EPO)去除土壤水分对高光谱的影响,利用偏最小二乘方法(PLSR)建立并对比EPO处理前、后不同SMC水平SOMC反演模型。结果表明,土壤水分对Vis-NIR光谱反射率有显著的影响,掩盖了SOMC的光谱吸收特征;EPO处理前不同SMC水平的光谱曲线之间的差异较为明显,而EPO处理后的各SMC水平的光谱曲线形态基本相似;采用EPO处理后的土壤高光谱数据建立SOMC估算模型,预测集的R2_p,RPD分别为0.84和2.50,其精度与EPO处理前所建模型相比有较大提升,表明EPO算法可以有效去除土壤水分的影响,从而提升SOMC的估算精度。对定向去除外部环境参数对土壤高光谱影响进行了实证,为完善野外原位获取SOMC信息技术提供理论基础。     

QuEChERS-超高效液相色谱-串联质谱法同时测定畜禽组织中6种黄曲霉毒素和杂色曲霉素

建立了QuEChERS前处理技术结合超高效液相色谱-串联质谱（UHPLC-MS/MS）检测畜禽组织中6种黄曲霉毒素和杂色曲霉素残留的分析方法。样品经20 μL β-葡萄糖苷酶酶解和10 mL乙腈-水（84：16,v/v）提取,然后用1.0 g氯化钠和1.0 g无水硫酸镁净化、5 mL正己烷脱脂,最后采用1 mL含5 mmol/L乙酸铵的乙腈-水溶液（80：20,v/v）复溶。以甲醇和5 mmol/L乙酸铵为流动相,在多反应监测正离子模式下进行检测,采用基质匹配标准曲线外标法进行定量分析。结果显示,6种黄曲霉毒素和杂色曲霉素在各自的范围内线性关系良好,相关系数（R²）>0.99,检出限（LOD,S/N=3）为0.007~0.30 μg/kg,定量限（LOQ,S/N=10）为0.02~0.91 μg/kg,加标回收率为77.3%~118.5%（n=6）。该法简单、快速,灵敏度高,适用于不同畜禽产品中6种黄曲霉毒素和杂色曲霉素的确证分析。