4,5-二苯基-2-(9-苯基-9H-3-咔唑基)-1H-咪唑的合成及其性能

以联苯甲酰、对溴苯甲醛和乙酸铵为原料，经缩合反应制得2-(4-溴苯基)-4,5-二苯基咪唑(1); 1与N-苯基-3-咔唑硼酸经Suzuki偶联反应合成了一种具有D-π-A结构的新型咪唑衍生物4,5-苯基-2-(9-苯基-9H-3-咔唑基)-1H-咪唑(2），其结构经¹H NMR, ¹³C NMR, IR和MS(EI)表征。采用FL, UV-Vis，循环伏安法（CV）和理论计算对2的光电性能进行了研究。结果表明：2的最大吸收波长为302 nm和326 nm，荧光发射波长为395 nm和412 nm, HOMO和LUMO轨道能级分别为5.35 eV和2.14 eV。     

双酚A人工抗原的光谱表征及免疫鉴定研究

双酚A(bisphenol A,BPA)是一种酚类环境雌激素,即使极低剂量残留对人类健康也有危害。为了建立其简单快速并适应现场检测的免疫分析方法,必须先合成它的人工抗原并进行鉴定。通过对双酚A进行结构修饰,用碳二亚胺将结构修饰了的双酚A与牛血清蛋白相偶联,经透析得到纯的双酚A人工抗原并冷冻干燥备用。然后用紫外光谱、红外光谱和免疫方法对人工抗原进行鉴定。结果表明,合成的人工抗原紫外光谱图中具有蛋白质和双酚A的特征吸收峰;人工抗原红外光谱图中呈现双酚A和牛血清蛋白的红外特征吸收峰;通过竞争ELISA试验表明用合成的人工抗原免疫的Balb/c小鼠血清中产生了双酚A的抗体。三种方法综合鉴定结果表明双酚A人工抗原合成成功,该人工抗原可以进一步用于制备抗双酚A的单克隆抗体。     

MR动态增强联合扩散加权成像技术在乳腺疾病诊断中的价值

目的探讨乳腺MR动态增强扫描联合扩散加权成像技术在乳腺疾病影像诊断中的价值。方法对48例女性乳腺患者进行MRI检查,先进行双侧乳腺常规MRI平扫,再进行双侧乳腺扩散加权平面回波（EPI）序列扫描,最后行乳腺动态增强扫描。通过后处理,得到病灶处表观弥散系数（ADC）值及时间-信号强度曲线。结果根据时间-信号强度曲线特点及ADC值,结合常规MRI形态学特点进行良、恶性病变诊断。MRI诊断为恶性肿瘤19例,良性病变29例。手术或活检病理结果证实MRI诊断为恶性肿瘤的19例患者中,18例为恶性肿瘤,另外1例为良性肿瘤（旺炽性腺瘤）;MRI诊断为良性病变的29例患者,手术或病理活检均证实为良性病变。MRI区分乳腺良、恶性病变的准确率为97.9%（47/48）,与手术或病理活检准确率（100%）比较差异无统计学意义（字2=0.044,P=0.834）。结论动态增强扫描联合扩散加权成像技术,可提高乳腺疾病影像诊断准确率。     

Crystal Structure and Bovine Serum Albumin Binding Studies of Methyl 4-(2,4-Dichlorophenyl)-2,7,7-trimethyl-5-oxo-1,4,5,6,7,8-hexahydroquinoline-3-carboxylate

The title compound, methyl 4-(2,4-dichlorophenyl)-2,7,7-trimethyl-5-oxo-1,4,5,6,7,8-hexahydroquinoline-3-carboxylate(C_(20) H_(21)Cl_2NO_3, 1) was synthesized and the crystal structure was —determined by single-crystal X-ray diffraction. It crystallizes in tetragonal system, space group P421 c with a = 16.076(3), b = 16.076(3), c = 14.750(2) ?, V = 3811.8(14) ?3, Z = 8, R = 0.0377 and wR = 0.0845. The interactions between compound 1 and bovine serum albumin(BSA) were investigated using fluorescence spectroscopy. The results revealed that compound 1 can effectively quench the intrinsic fluorescence of BSA by static quenching mechanism. The thermodynamic parameters revealed that the action forces between compound 1 and BSA were mainly van der Waals forces and hydrogen bonds. According to F?rster's non-radioactive energy transfer theory, the binding distance between compound 1 and BSA had been determined. Furthermore, the synchronous fluorescence showed that compound 1 has few effects on the microenvironment and conformation of BSA in the binding process.