碳酸酐酶Ⅱ在天然产物生物活性筛选研究中的应用--结构、功能、活性筛选、抑制剂及其相互作用

碳酸酐酶(carbonic anhydrases,CAs,EC 4.2.1.1)是一系列含锌的金属酶,它能可逆性地催化二氧化碳的水合反应:CO2 H2O〈＝〉HCO3- H .目前可能除了真菌以外的其它生物体中都已发现该类酶的存在.到目前为止所发现的碳酸酐酶主要可以分为α,β,γ,δ四类,其中哺乳动物中的碳酸酐酶均属于α类碳酸酐酶;人类的碳酸酐酶家族包括7种同工酶,分别称为hCA Ⅰ-hCA Ⅶ,其中研究最广、与人类关系最密切的是碳酸酐酶Ⅱ(hCA Ⅱ).碳酸酐酶Ⅱ的分子量约为30 kDa,它的活性中心主要由两部分组成:一是在配基His-94,His-96,His-119,Thr-199,Glu-106,His-64参与下Zn2 与H2O或OH-形成的多面体;二是在上述多面体的邻近处有一个由Leu-198,Val-143,Val-121,Trp-209组成的结合有CO2的疏水袋.由于该酶在体内多种器官、组织中都有分布,故其催化CO2水合生成H 的作用与体内多种生理或病理过程有关,如人体酸碱平衡、青光眼、骨质疏松症、癫痫病等.由于碳酸酐酶Ⅱ涉及人类多种疾病,因此寻找其抑制剂已逐渐成为热点,目前报道的抑制剂主要有磺胺类如乙酰唑胺等.     

Dynamical Convergence Trajectory in Networks

It is well known that topology and dynamics are two major aspects to determine the function of a network. We study one of the dynamic properties of a network： trajectory convergence, i.e. how a system converges to its steady state. Using numerical and analytical methods, we show that in a logical-like dynamical model, the occurrence of convergent trajectory in a network depends mainly on the type of the fixed point and the ratio between activation and inhibition links. We analytically proof that this property is induced by the competition between two types of state transition structures in phase space： tree-like transition structure and star-like transition structure. We show that the biological networks, such as the cell cycle network in budding yeast, prefers the tree-like transition structures and suggest that this type of convergence trajectories may be universal.     

神光-Ⅲ主机装置下装模块空间对接关键技术北大核心CSCD

初步分析了在建神光-Ⅲ主机装置下装模块空间对接过程,指出"对中"和"碰撞"问题是下装模块空间对接监测的关键技术。"对中"监测技术主要是将三维问题转换为二维问题,利用机器视觉扑捉特征点下装模块进行位置和姿态的判断。"碰撞"监测技术主要是利用下装模块装校过程发生碰撞必然产生"力"的作用的原理,监测碰撞点上各个方向作用力,间接反映碰撞发生程度,并将测量数据分析后反馈至对接平台执行,及时修正下装模块空间对接运动轨迹,实现了下装模块全过程的"轻碰撞"对接。     

沉淀法合成纳米晶上转换发光材料Y_2O_2S:Yb,Er

采用沉淀法在不同温度下合成了纳米上转换发光材料Y2O2S∶Yb,Er,运用XRD、TEM和上转换发光光谱对其进行表征。结果表明,使用该法在700℃即能合成纳米上转换发光材料Y2O2S∶Yb,Er,随着合成温度的升高,产物的粒径从60到120nm逐渐增大。上转换发光光谱显示该材料主要有2个发射带,其中红光发射的中心波长位于668nm,绿光发射的中心波长位于525和550nm。此外,对材料的上转换发光过程进行了探讨。     

V(Ⅳ)/V(Ⅴ)电对在石墨毡与导电塑料复合电极上的反应机理(英文)

应用循环伏安、极化曲线和交流阻抗等电化学方法研究了V(Ⅳ)/V(Ⅴ)电对在石墨毡复合电极上反应的速控步骤.结果表明,V(Ⅳ)/V(Ⅴ)电对在石墨毡电极上的反应属准可逆过程,且氧化过程包含有后置化学转化步骤;该过程Tafel斜率的实验值为0.124,而理论计算的,以电化学步骤作为控制步骤的Tafel斜率约0.12,两者吻合很好,表明该氧化过程受电化学步骤控制;以等效电路拟合不同极化电位下的交流阻抗,得出该电化学反应阻抗远大于其他阻抗,意味着电化学过程可能是电极反应的控制步骤,与实验得到的极化曲线分析结果相一致.     

The isolated critical value phenomenon in local－global riddling bifurcation

A chaotic synchronized system of two coupled skew tent maps is discussed in this paper. The locally and globally riddled basins of the chaotic synchronized attractor are studied. It is found that there is a novel phenomenon in the local－global riddling bifurcation of the attractive basin of the chaotic synchronized attractor in some specific coupling intervals. The coupling parameter corresponding to the locally riddled basin has a single value which is embedded in the coupling parameter interval corresponding to the globally riddled basin, just like a breakpoint. Also, there is no relation between this phenomenon and the form of the chaotic synchronized attractor. This phenomenon is found analytically. We also try to explain it in a physical sense. It may be that the chaotic synchronized attractor is in the critical state, as it is infinitely close to the boundary of its attractive basin. We conjecture that this isolated critical value phenomenon will be common in a system with a chaotic attractor in the critical state, in spite of the system being discrete or differential.     

汽液界面厚度和分维数

本文应用分子动力学模拟方法进行了汽液界面厚度和分维数的模拟研究。用统计系综方法,以氩原子为对象,对长方形模拟盒中粒子数目为2048个的汽液平衡共存系统进行模拟计算。研究了一定条件下汽液界面厚度的取定对确定界面分维数的影响,根据计算模拟结果,提出了可用15~85法则确定界面厚度和分维数。汽液界面在X方向、Y方向和Z方向具有不同的分维数,充分说明了汽液界面的各性异性性质。     

透明质酸酶的色氨酸残基修饰与荧光光谱研究

北五味子[Schisandra chinensis（Turcz．）Baill．]属广义木兰科植物,主产于我国东北,故又称“辽五味”,中药五味子的主要药材为北五味子的干燥果实,作为一种传统中药,五味子具有收敛固涩,益气生津,补肾宁心的功效,用于肺喘虚咳,心悸失眠诸病。     

沉淀法合成蓝色长余辉发光材料Sr2MgSi2O7∶Eu2+，Dy3+

采用沉淀法制备了高亮度的长余辉发光材料Sr₂MgSi₂O₇∶Eu²⁺,Dy³⁺。通过XRD、荧光光谱和热释光谱对其进行表征。XRD测试表明所制备的Sr₂MgSi₂O₇∶Eu²⁺,Dy³⁺为单相,四方晶。荧光光谱测试表明,用λ_em=467 nm作为监控波长,在275~450 nm之间有宽的激发光谱,峰值位于399 nm。用λ_ex=399 nm激发样品,其发射光谱为一宽带,峰值位于467 nm。1 050 ℃煅烧前躯体所制备的Sr₂MgSi₂O₇∶Eu²⁺,Dy³⁺发光性能最好。热释光谱峰值位于357 K,适合长余辉现象的产生。对Sr₂MgSi₂O₇∶Eu²⁺,Dy³⁺长余辉发光机理进行了讨论。