层粘连蛋白受体的人工移植及其在小鼠Lewis肺癌实验性转移中之作用的研究

本文报道将从小鼠Lewis肺癌组织分离的层粘连蛋白受体(laminin receptor,LN-R),经~(125)I标记,重组入脂质体,用Sepharose 4B柱层析分离重组LN-R的脂质体,借助聚乙二醇的诱导,将LN-R脂质体与Lewis肺癌细胞融合,以达到受体的人工移植,融合后细胞带有盐溶液不能去除的放射性,与层粘连蛋白(laminin,LN)基质的结合率较对照组高87.5％。移植LN-R的癌细胞经尾静脉注入小鼠体内后,使小鼠死亡时间提前,肺连同转移肺癌组织总重量较对照组显著增加。提示LN-R是参与癌转移过程的重要生物分子。     

Generation and Quantum Interference of Entangled Electron-Hole Pairs in a Hanbury Brown and Twiss Interferometer

The Hanbury Brown-Twiss interferometer is proposed to serve as a detector of the crossed Andreev reflection and to generate entangled electron-hole pairs. It is shown that the non-local electron and hole induced by the crossed Andreev reflection are entangled. Quantum interference of the entangled electron-hole pairs gives rise to a new measurable effect of the phase difference between two superconductors in the cross correlation. The present theoretical predictions can be experimentally realized within the present-day microelectronic technology.     

运动之中的常规考量--2014年广东卷中考第25题的思路突破与教学建议

研读2014年广东省中考数学试卷,在保持总体难度稳定平衡的基础上,试卷结构更趋合理,基础知识覆盖面更广,特别是压轴题的设计,一如既往地以动态变化类为基架,突出考查学生在未来数学学习和应用数学解决问题过程中最为重要的核心概念、思想方法和常用的技能,综合性更强,具有较高的信度和恰当的区分度。本文拟对该压轴题展开一些思路探究,并围绕该题的解题教学给出建议,供同行研讨。     

Novel technique for characterizing feature profiles in photolithography process

A novel angle-resolved scatterometer based on pupil optimization for feature profile measurement in a photolithography process is proposed. The impact of image sensor errors is minimized by optimizing the intensity distribution of the incident light using a spatial light modulator. The scatterometry sensitivity of feature measurement at different polarization conditions is calculated using the rigorous coupled-wave and first-order analyses, and the reproducibility of the scatterometer is evaluated. The results show that the sensitivity and reproducibility of the angle-resolved scatterometer increase by 90% and 40% with pupil optimization, respectively.     

新型极地船用钢在模拟低温海水中的磨损-腐蚀耦合作用研究

分别采用正火和调质工艺对新型破冰船用低碳高强钢进行了热处理，并使用UMT-3多功能摩擦磨损试验机低温模块和摩擦电化学模块研究了其在低温模拟海水环境中不同外加电位下的磨损-腐蚀耦合行为. 使用电子扫描显微镜和白光干涉显微镜对钢样的微观组织及磨痕形貌进行了表征，并对其在不同外加电位作用下的磨损-腐蚀机理进行了探讨. 结果表明:两种热处理后的钢材的平均摩擦系数都随外加电位升高而降低，而磨痕深度、磨损量和腐蚀电流则随着外加电位升高而增加；钢材的开路电位随着磨损的发生逐渐负移，腐蚀电流密度增加；其中，经过正火处理的钢样磨痕表面主要出现剥落坑，而调质钢磨痕表面则主要出现了裂纹和腐蚀坑. 正火钢磨损和腐蚀损失量所占比例分别为80.6%、19.4%，调质钢分别为55.1%、44.9%，两种钢材的磨损-腐蚀之间相互促进损失量ΔV_W和ΔV_C均为正值，证实磨损和腐蚀之间存在着相互协同作用.      

STUDIES ON TRANSPLANTATION OF THE LAMININ RECEPTOR AND ITS ROLES IN EXPERIMENTAL METASTASIS OF MURINE LEWIS LUNG CARCINOMA CELLS

The isolated laminin receptor (LN-R) labeled by ~(125)I was reconstituted into liposomes.~(125)I-LN-R-liposomes and free ~(125)I-LN-R were separated by Sepharose 4B column chromatogra-phy. The LN-R-liposomes showed affinity for laminin (LN) and were capable of binding toimmobilized LN substrate. In order to make transplantation of LN-R, LN-R-liposomes werefused with cultured murine Lewis lung carcinoma cells with the help of polyethylene glycol(PEG) induction. The radiation with the fused cells was not removed by salt sclution. Thebinding of the fused cells enriched in foreign LN-R to LN substrate increased by 87.5%.Furthermore, the murine Lewis lung carcinoma cells with and without transplanted LN-Rwere injected into C_(57)BL/6J mice through tail veins (5×10~5 cells/each mouse)respectively.The mice in the test group died earlier than those in the control group. The total weight oflung tumor in the test group remarkably increased in comparison with those in the controlgroup. The results taken together