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With 40 years of development, bio-macromolecule cryo-electron microscopy(cryo-EM) has completed its revolution in terms of resolution and currently plays a highly important role in structural biology study. According to different specimen states, cryo-EM involves three specific techniques: single-particle analysis(SPA), electron tomography and subtomogram averaging, and electron diffraction. None of these three techniques have realized their full potential for solving the structures of bio-macromolecules and therefore need additional development. In this review, the current existing bottlenecks of cryo-EM SPA are discussed with theoretical analysis, which include the air–water interface during specimen cryo-vitrification, bio-macromolecular conformational heterogeneity, focus gradient within thick specimens, and electron radiation damage. Furthermore, potential solutions of these bottlenecks worthy of further investigation are proposed and discussed. 相似文献
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新课标要求初中数学教学要创设积极、愉快的教学氛围,调动学生学习数学的兴趣,提高学习数学的积极性,让学生在愉悦的情境下,积极主动、轻松愉悦地参与学习,让他们从多个角度思考探究数学题的奥秘,由被动变为主动学习,实现数学学习的高效性. 相似文献
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许多中国公司无法通过IPO海外上市,转而谋求买壳上市。福麟国际大胆创新买壳上市之路,采用IPL模式成功登陆纳斯达克,个中操作技巧颇值得借鉴。 相似文献
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Using the effective Hamiltonian method, we analyze the B0-B0 mixing in the extension of the standard model (SM) where baryon number and lepton number are local gauge symmetries. The numerical results indicate the correction from the extra particles to the mass difference ArnB is significant. There is a 60% enhancement compared to the SM prediction for AraB at most, which agrees with the current experimental result. 相似文献
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随着器件结构与制作工艺的不断创新与完善,硅基发光器件已经可以实现室温下的有效工作,外量子效率可达到0.1%;低功耗的硅基高速调制器件的调制速率达到1GHz以上;而硅基光探测器对1300nm与1550nm波长的探测响应度也已分别达到了0.16mA/W和0.08mA/W.文章对硅基光电器件的研究进展情况进行了概述,并着重对几种器件的结构及工作原理进行了分析. 相似文献