基于人工免疫网络的药代动力学参数优化方法

提出了基于人工免疫网络优化药代动力学参数的PKAIN算法．新增的分组并发单纯形变异用以提高人工免疫网络的局部搜索能力．通过PKAIN人工免疫网络中网络细胞的进化得到给定药代动力学模型的一组优化参数．应用Laplace变换求解瑞芬太尼(remifentanil)及其代谢产物瑞芬太尼酸的联合代谢动力学模型的微分方程组,通过PKAIN算法优化导出房室模型参数A·D2实验表明,对伴有轻度肾损伤病人可以应用二室模型描述瑞芬太尼酸的药代动力学特征．     

表面接枝亲水性聚合物刷磁性微球的制备及其对蜂蜜中四环素类抗生素残留的磁分散固相萃取

采用电子转移活化再生催化剂原子转移自由基聚合法（ARGET-ATRP）在磁性微球（MMs）表面连续接枝了聚碱类聚合物和聚乙二醇刷。首先将MMs包覆硅胶,接枝氨基,然后在其表面接枝溴引发剂,最后在MMs表面进行聚合,制备了亲水性聚合物刷磁性微球（HMMs）。通过透射电镜、傅里叶变换红外光谱对HMMs进行表征,并研究了HMMs对蛋白质的吸附性能。结果表明,合成的HMMs粒径较为均一,分散性良好并且具有良好的抗蛋白质吸附性能。利用制备的HMMs,采用磁分散固相萃取（MDSPE）-高效液相色谱法（HPLC）测定了蜂蜜中四环素类抗生素（TCs）残留。TCs的平均回收率为85.8%~94.5%,方法的检出限和定量限分别为1.92~2.56 μg/kg和6.40~8.53 μg/kg。该方法成功地用于蜂蜜中TCs残留的快速分离富集。     

Parameter optimization of pharmacokinetics based on artificial immune network

A new method for parameter optimization of pharmacokinetics based on an artificial immune network named PKAIN is proposed. To improve local searching ability of the artificial immune network, a partition-based concurrent simplex mutation is developed. By means of evolution of network cells in the PKAIN artificial immune network, an optimal set of parameters of a given pharmacokinetic model is obtained. The Laplace transform is applied to the pharmacokinetic differential equations of remifentanil and its major metabolite, remifentanil acid. The PKAIN method is used to optimize parameters of the derived compartment models. Experimental results show that the twocompartment model is sufficient for the pharmacokinetic study of remifentanil acid for patients with mild degree of renal impairment.