HPLC测定排石颗粒中芦丁含量的不确定度评定

分析HPLC法测定排石颗粒中芦丁含量的不确定度.通过建立HPLC法测定含量的数学模型,找出影响不确定度的因素,并对各个不确定度因素进行评估、计算,由此计算合成不确定度,最终给出测量结果的扩展不确定度和置信水平.合成不确定度为0.026；扩展不确定度为0.007％；扩展不确定度为0.007％,排石颗粒含量/％测定结果为(0.13士0.007)％.建立HPLC法的不确定度分析与计算方法适用于颗粒制剂HPLC法含量测定不确定度评定.     

STUDIES ON BRADYKININ POTENTIATING PEPTIDE (BPP) FROM VENOM OF ZHEJIANG PIT VIPER (Agkistrodon halys pallas)——RELATIONSHIP BETWEEN STRUCTURE AND FUNCTION OF BPP_1

1 The structure of the bradykinin potentiating peptide component BPP_1 from a 70% methanol extract of the lyophilyzed powder of pit viper from Zhejiang Province is shown as follows: Glu·Gly·Arg·Pro·Pro·Gly·Pro·Pro·Ile·Pro·Pro2 When the pyroglutamyl residue was removed by pyroglutamate aminopeptidase, the activity of BPP_1 was enhanced two-fold but decreased gradually during the course of Edman degradation. The above facts show that the structure integrity of the C-terminal is absolutely necessary for the activity of BPP_1.     

活性多肽的概况

活性多肽是指一些在体内含量极微而又具有特殊生理功能的多肽类物质,它是由某些腺体细胞或神经元所分泌,也可由血液中的α球蛋白经专一酶作用后所释放的。当它们在体内分泌失常或代谢失调,就导致严重的病变。因而,对活性多肽的研究不仅有极重要的理论意义,更有着广泛的实际意义。由于活性多肽在体内含量极微(一般在10~(-9)克/毫升体液的水平),取材困难,再加上很难找到简便而又专一的检测方法,因而这方面的进展曾受到一定的限制。美国的Schally及Guillemin两实验室从事下丘脑多     

COMPLETE AMINO ACID SEQUENCE OF MUNG BEAN TRYPSIN INHIBITOR

The mung bean trypsin inhibitor has been found to be microheterogeneous at N-terminalregion due to the presence of several isomers. After treatment with aminopeptidase M it becomeshomogeneous and is suitable for sequence determination. Based on the determination of the structures of two active fragments the complete aminoacid sequence of mung bean trypsin inhibitor has been elucidated. It consists of 72 amino acidresidues with 7 pairs of disulfide bonds. The results show that this inhibitor belongs to theBowman-Birk inhibitor family.     

文蛤糖肽MGP_(0501)体内抗肿瘤活性

对文蛤中分离得到的一种新糖肽MGP0501进行体内抗肿瘤活性评价和免疫调节功能研究。采用移植性肿瘤研究法接种,考察MGP0501对S180肉瘤、EAC腹水瘤和肝癌Heps小鼠的瘤组织的影响;通过对不同器官组织的毒性实验,考察MGP0501抗肿瘤的可能机制。MGP0501浓度为6mg·kg-1时,对S180和Heps抑瘤率分别为69.72%和66.43%,对EAC瘤生命延长率为15.91%,显著高于Cy抗肿瘤作用;MGP0501浓度为150μg·mL-1时,对正常小鼠脾淋巴细胞的刺激指数是1.21,对移植瘤S180和Heps小鼠的免疫器官有显著增强作用。MGP0501是一种新型的抗肿瘤糖肽,在体内具有稳定的抗肿瘤作用和免疫调节功能。