Association Behavior of 4-Acylpyrazolone Derivative and Tertiary Amine of High Molecular Weight in Antagonistic Synergistic Extraction of Palladium

To find an effective extraction and removal method for palladium(II), which is one of the main fission products from an acidic nuclear spent fuel solution, the extraction behavior of palladium(II) from a nitric acid medium by an acidic chelating extractant, 1-phenyl-3-methyl-4-trifluoroacetylpyrazolone-5-one (HPMTP) and a tertiary amine of high molecular weight, tri-n-octylamine (TOA), has been studied by spectrophotometry. A noticeable antagonistic extraction effect was observed in the extraction system under the given conditions. To understand this phenomenon, a preliminary investigation was performed to explain the mechanism of this reaction. According to the theory of corresponding solutions (TCS), the association reaction between HPMTP and TOA is proposed in the organic phase. An associated species, HPMTP·TOA, formed through hydrogen bonding in a chloroform medium might be the main reason why an antagonistic extraction effect occurred. The association constant between HPMTP and TOA was calculated to be 2.86±0.05.     

2-Deoxy-d-glucose Promotes Buforin IIb-Induced Cytotoxicity in Prostate Cancer DU145 Cells and Xenograft Tumors

Inhibition of the glycolytic pathway is a critical strategy in anticancer therapy because of the role of aerobic glycolysis in cancer cells. The glycolytic inhibitor 2-Deoxy-d-glucose (2-DG) has shown potential in combination with other anticancer agents. Buforin IIb is an effective antimicrobial peptide (AMP) with broad-spectrum anticancer activity and selectivity. The efficacy of combination treatment with 2-DG and buforin IIb in prostate cancer remains unknown. Here, we tested the efficacy of buforin IIb as a mitochondria-targeting AMP in the androgen-independent human prostate cancer cell line DU145. Combining 2-DG with buforin IIb had a synergistic toxic effect on DU145 cells and mouse xenograft tumors. Combination treatment with 2-DG and buforin IIb caused stronger proliferation inhibition, greater G1 cell cycle arrest, and higher apoptosis than either treatment alone. Combination treatment dramatically decreased L-lactate production and intracellular ATP levels, indicating severe inhibition of glycolysis and ATP production. Flow cytometry and confocal laser scanning microscopy results indicate that 2-DG may increase buforin IIb uptake by DU145 cells, thereby increasing the mitochondria-targeting capacity of buforin IIb. This may partly explain the effect of combination treatment on enhancing buforin IIb-induced apoptosis. Consistently, 2-DG increased mitochondrial dysfunction and upregulated Bax/Bcl-2, promoting cytochrome c release to initiate procaspase 3 cleavage induced by buforin IIb. These results suggest that 2-DG sensitizes prostate cancer DU145 cells to buforin IIb. Moreover, combination treatment caused minimal hemolysis and cytotoxicity to normal WPMY-1 cells. Collectively, the current study demonstrates that dual targeting of glycolysis and mitochondria by 2-DG and buforin IIb may be an effective anticancer strategy for the treatment of some advanced prostate cancer.     

毛细管区带电泳技术快速分离检测爆炸残留物中的无机离子

利用间接紫外毛细管区带电泳方法完成了对爆炸残留物中7种无机离子(K+,NH+4,NO-2,NO-3,SO2-4,ClO-3,ClO-4)的分离检测。阳离子测定采用的缓冲体系为10 mmol/L吡啶（pH 4.5）-3 mmol/L冠醚,K+和NH+4在2.6 min内达到基线分离,检出限分别为0.25 mg/L和0.10 mg/L(S/N=3)。阴离子测定采用的缓冲体系为40 mmol/L硼酸-1.8 mmol/L重铬酸钾-2 mmol/L硼酸钠（pH 8.6）,氢氧化四甲铵为电渗流改性剂,5种阴离子在4.6 min内达到基线分离,检出限为0.10～1.85 mg/L。该方法已成功地应用于实际爆炸物样品种类的判定分析,取得了很好的结果。     

黏性边界层网格自动生成

高雷诺数黏性流动在壁面附近存在边界层,在计算模拟中自动生成可靠且有效的计算网格仍然是计算流体力学存在的瓶颈.三棱柱/四面体混合网格技术在一定程度上缓解了这个困难.然而,对于复杂外形的情况,在边界层内自动高效生成高质量的三棱柱单元仍然十分困难.常用的层推进法在凹凸区域及角点处生成的边界层网格单元质量较差,边界层网格最外层尺寸不均匀.针对这些问题,发展了一种黏性边界层网格自动生成方法,通过顶点周围边的二面角识别物面网格特征确定多生长方向,预估并调整生长高度处理相交情况.同时提出一种三维前沿尺寸调节方式,提高了边界层网格单元的正交性,保证了边界层网格与远场网格尺寸的光滑过渡.通过ONERA M6翼型以及带发动机短舱的DLR-F6翼身组合体等外形的网格生成实例及绕流数值模拟,将计算值与标准实验值进行对比,结果表明:该方法能够自动高效地生成满足数值计算需求的混合网格.     

毛细管区带电泳技术快速分离检测爆炸残留物中的无机离子

利用间接紫外毛细管区带电泳方法完成了对爆炸残留物中7种无机离子(K+,NH+4,NO-2,NO-3,SO2-4,ClO-3,ClO-4)的分离检测。阳离子测定采用的缓冲体系为10 mmol/L吡啶（pH 4.5）-3 mmol/L冠醚,K+和NH+4在2.6 min内达到基线分离,检出限分别为0.25 mg/L和0.10 mg/L(S/N=3)。阴离子测定采用的缓冲体系为40 mmol/L硼酸-1.8 mmol/L重铬酸钾-2 mmol/L硼酸钠（pH 8.6）,氢氧化四甲铵为电渗流改性剂,5种阴离子在4.6 min内达到基线分离,检出限为0.10～1.85 mg/L。该方法已成功地应用于实际爆炸物样品种类的判定分析,取得了很好的结果。     

运载火箭结构动特性分析工具的设计与实现

运载火箭的结构模型复杂,只有通过开发专用的仿真分析软件,才能高效、便捷的对其开展动力学特性分析。为保证工具建设的实用性,该软件基于航天领域应用广泛的Patran/Nastran软件进行二次开发。针对仿真分析流程,设计实现了运载火箭结构动特性分析工具,涵盖了建模与计算模块、结果后处理模块、模态筛选模块与模型修正模块,并提炼了其中的关键技术。经工程实践表明,工具可提升设计效率,且计算结果准确、通用性与扩展性强,在工程中具备良好的应用价值。     

希土配合物的研究——Ⅷ.1, 4-双(1''-苯基-3''-甲基-5''-氧代吡唑酮基-4'')代-1, 4-丁二酮(BPMPBD)与希土元素配合物的合成及表征

在乙醇-水溶液中,当pH=5-6时,用希土硝酸盐与BPMPBD反应,合成了15种希土元素(除Sc、Pm外)的二元配合物.通过化学分析和元素分析确定了配合物的组成为REL₂·nH₂O(RE=La,n=5,RE=Y,n=4,RE=Pr、Nd、Sm、Eu、Gd,n=3),RE₂L₃·5H₂O(RE=Tb、Dy、Ho、Er、Tm、Yb、Lu)及CeL₂·4H₂O.研究了这些配合物的一些性质及红外光谱、紫外光谱、核磁共振、荧光光谱和差热分析,认为重希土配合物具有双核结构.     

Gene function classification using NCI-60 cell line gene expression profiles

Gene expression patterns from NCI's panel of 60 cell lines were used to train a Neural Network model for classifying genes to pathways. The model assigns probabilities to each gene for each of the 21 modeled pathways assigned by the Kyoto Encyclopedia of Genes and Genomes. Cross-validation of the model showed that 10 of the 21 pathways exhibited good performance in statistical significance and accuracy. The model was designed to output gene probabilities that could be screened for higher probabilities resulting in higher confidence in classification though yielding fewer genes per pathway. The model was deployed on 5798 genes and our approach allowed us to ascertain the most relevant genes above an estimated background. Eight pathways were identified with both good cross-validation and significant numbers above background, TCA Cycle, Oxidative Phosphorylation, Porphyrin Biosynthesis, Ribosome, Polymerases, Proteasome, Cell Cycle, and Cell Adhesion. Gene Ontology (GO) annotation was used for additional validation of gene classification results. A total of 551 GO annotated genes and 468 unannotated genes were classified to the 8 pathways. The primary and secondary classifications of genes revealed known pathway relationships and provide the potential for discovering new pathway relationships.     

Periodic self-triggered intermittent sampled-data stabilization for stochastic complex networks

In this paper, a new class of periodic self-triggered intermittent control with sampled-data (PSICS) is designed to tackle the stabilization issue for stochastic complex networks with time delays and Lévy noise (SCNTL). Therein, the self-triggered scheme is propounded with regard to intermittent control which is periodic judgment, and there exists a sampled-data control in every intervals of periodic judgment in the control time (work time) of intermittent control. It is worth pointing out that PSICS possesses more flexibility in terms of applications and simpler design of triggered conditions, compared with some previously reported control strategies such as the control combines the advantages of the periodic sampling and self-triggered control. Meanwhile, by means of stability analysis, sampled-data control, intermittent control and event-driven control theory, a useful criterion is established to guarantee the exponential stability in mean square of SCNTL. Notably, the stabilization issue of single-link robot arms with time delays and Lévy noise via PSICS is studied, as a practical application of SCNTL. Ultimately, numerical simulations are utilized for illustration.