O～3岁儿童微量元素检测及纠正150例观察与体会

对西安地区150例0～3岁儿童进行了六种微量元素：钙、锌、铜、铁、镁、硒的检测，发现微量元素缺乏可导致急慢性感染、免疫力低下、小儿营养不良、贫血、发育迟缓等症状。并对小儿营养不良、喂养不当进行矫正。其中铁、钙、锌同时缺乏占50％。     

新铜试剂—曙红Y萃取光度法测定微量铜

铜(I)-新铜试剂(NCP)-曙红Y(Eosin Y)能形成离子对三元络合物,其络合比为1:2:1。在氯仿中该络合物的λ_(max)=544nm,表观摩尔吸光系数ε=6.83×10~4 L mol~(-1)cm~(-1)。以柠檬酸盐作掩蔽剂,控制水相pH=4.5,相比(水相/氯仿)=2/1,可将络合物定量萃入氯仿中。一般常见的共存离子均不干扰测定,显色稳定性好,线性范围为0～5μg cu/5ml氯仿。方法灵救度高,选择性好。     

Two New Sesquiterpenes from Eupatorium lindleyanum

Various germacrane sesquiterpenes have been isolated from many species of the Eupatorium.In recent years these compounds have been increasing interest due to their insecticidal,cytotoxic,antitumor-promoting and insect-antifeedant activities1.In our present study,we have investigated the chemical constituents of Eupatorium lindleyanum DC.,which is a geo-authentic medicine of Jiangsu province.It is used as a antipyretic drug.Two novel germacrane sesquiterpenes(Figure1)were found by us from th…     

Fusion Wheat Histone H4 Protein Increases Transfection Efficiency of Non-viral DNA Vector

The lack of efficient and non-toxic gene delivery, preferably with non-viral DNA vectors, is generally regarded as a major limitation for gene therapy. In this study, a wheat histone H4 gene was cloned from Triticum aestivum, sequenced, modified and expressed in E. coli. The wheat histone H4 gene and reconstructed H4TL gene encoded wheat histone H4 and a recombinant protein of 141 amino acids with an approximate molecular weight of 15500. Gel electrophoresis mobility shift assays demonstrated that the purif...     

Swallowtail model for predicting the global bifurcation behavior of CO oxidation reactions

Catalytic CO oxidation on platinum group metals exhibits nonlinear phenomena such as hysteresis and bifurcation.Elucidation of the nonlinear mechanisms is important for catalyst design and control of reaction routes.Previous studies have offered initial insights into the local bifurcation behavior of CO oxidation.However,since the bifurcation behavior of CO oxidation is determined by multiple parameters such as temperature,total flux,and CO fraction,it is difficult to predict the global bifurcation behavior...     

Adjuvant Effects of Dual Co-stimulatory Molecules on Cellular Responses to HIV Multiple-epitope DNA Vaccination

Designing adjuvants that can induce strong cytotoxic T cell responses is largely required for preparing DNA vaccines. In this study we explored dual costimulatory molecules 4-1BBL and OX40L as adjuvants to improve the efficiency of the HIV multiple-epitope DNA vaccine. When explored in the human dendritic cell-T cell based coculture system,dual costimulatory molecules significantly enhanced the anti-HIV T cell response of the HIV mul-tiple-epitope DNA vaccine,as detected by intracellular cytokine staining t...     

Synthesis of new chiral 1,2,3,4-tertrahydroisoquinoline β-amino alcohol for asymmetric diethylzinc addition to aryl aldehydes(Ⅰ)

Four new chiral 1,2,3,4-tetrahydroisoquinoline-derived β-amino alcohols were synthesized from commercially available L-DOPA. These ligands were evaluated in the asymmetric addition of diethylzinc to benzaldehydes and showed different catalytic activities(up to 86%ee).The solvent played an important role in the enantioselective process.The transition state models were proposed to explain the reversion of the product configuration.     

Screening and Identifying of Nephrotoxic Compounds in Lithospermum erythrorhizon Using Live-cell Fluorescence Imaging and Liquid Chromatography Coupled with Tandem Mass Spectrometry

In order to identify the potential nephrotoxic compounds in traditional Chinese medicine Lithospermum erythrorhizon,it was separated into serial fractions according to their polarities.An in vitro method was utilized to determine the nephrotoxicity of these fractions with the help of fluorescence image analysis.As a result,the primary fraction A05 and its secondary fractions C06 C09 and C12 C14 were found to have significant toxicity to LLC-PK1 cell line,as determined by the survive rate less than 20% after...     

An unusual bioactive oleanane triterpenoid from Rhododendron campanulatum D.Don

An unusual oleanane triterpene(1) was isolated from aerial parts of Rhododendron campanulatum.The compound had shown prominent antibacterial and immunomodulatory activities.The structure of the compound was determined by spectroscopic data including 1D and 2D NMR spectral analysis.     

Components of Essential Oils in Different Parts of Daucus carota L. var. sativa Hoffm

Introduction Carrots,avegetablewithabundantnutrition,are therootsofDaucuscarotaL.var.sativaHoffm[1],whichisanumbelliferaeplant.Carrotsshowanti can cerandanti oxidationactivities.Theycanalsobeused topreventeyediseasesandimproveimmunity[2—11],thefactsareun…