Large inclusion constants of ��-cyclodextrin with carborane derivatives

Complexation of some o-, m- and p-carborane derivatives with ??-cyclodextrin was investigated using phenolphthalein in pH 10.5 (0.05 mol dm^?3) borate buffer. Some carborane derivatives indicated large inclusion constants K_ass > 1 × 10⁵ dm³ mol^?1.     

Magneto-mechanical trapping systems for biological target detection

We demonstrate a magnetic microsystem capable of detecting nucleic acids via the size difference between bare magnetic beads and bead compounds. The bead compounds are formed through linking nonmagnetic beads and magnetic beads by the target nucleic acids. The system comprises a tunnel magneto-resistive (TMR) sensor, a trapping well, and a bead-concentrator. The TMR sensor detects the stray field of magnetic beads inside the trapping well, while the sensor output depends on the number of beads. The size of the bead compounds is larger than that of bare magnetic beads, and fewer magnetic beads are required to fill the trapping well. The bead-concentrator, in turn, is capable of filling the trap in a controlled fashion and so to shorten the assay time. The bead-concentrator includes conducting loops surrounding the trapping well and a conducting line underneath. The central conducting line serves to attract magnetic beads in the trapping well and provides a magnetic field to magnetize them so to make them detectable by the TMR sensor. This system excels by its simplicity in that the DNA is incubated with magnetic and nonmagnetic beads, and the solution is then applied to the chip and analyzed in a single step. In current experiments, a signal-to-noise ratio of 40.3 dB was obtained for a solution containing 20.8 nM of DNA. The sensitivity and applicability of this method can be controlled by the size or concentration of the nonmagnetic bead, or by the dimension of the trapping well.

If biological targets are present, they link magnetic beads and fluorescent beads. This results in less magnetic beads to be on the surface of magnetic sensor, causing a smaller signal, thus biological targets are detected.     

Resonant gravimetric immunosensing based on capacitive micromachined ultrasound transducers

High-frequency (40 MHz) and low-frequency (7 MHz) capacitive micromachined ultrasound transducers (CMUT) were fabricated and tested for use in gravimetric detection of biomolecules. The low-frequency CMUT sensors have a gold-coated surface, while the high-frequency sensors have a silicon nitride surface. Both surfaces were functionalized with bovine leukemia virus antigen gp51 acting as the antigen. On addition of an a specific antibody labeled with horseradish peroxidase (HRP), the antigen/antibody complex is formed on the surface and quantified by HRP-catalyzed oxidation of tetramethylbenzidine. It has been found that a considerably smaller quantity of immuno complex is formed on the high frequency sensor surface. In parallel, the loading of the surface of the CMUT was determined via resonance frequency and electromechanical resistance readings. Following the formation of the immuno complexes, the resonance frequencies of the low-frequency and high-frequency sensors decrease by up to 420 and 440 kHz, respectively. Finite element analysis reveals that the loading of the (gold-coated) low frequency sensors is several times larger than that on high frequency sensors. The formation of the protein film with pronounced elasticity and stress on the gold surface case is discussed. We also discuss the adoption of this method for the detection of DNA using a hybridization assay following polymerase chain reaction. Figure

Resonant gravimetric immunosensing with six-channel, 7 MHz capacitive micromachined ultrasound transducer (CMUT). Initial modification by antigen gp51 builds the protein layer with 13.2 GPa elasticity modulus. This increases the resonance frequency of CMUT, which is decreasing after immune complex with antibody is established.     

Clustering Effect on the Statistical Estimation Accuracy of Distribution Density

The paper is devoted to statistical nonparametric estimation of multivariate distribution density. The influence of data pre-clustering on the estimation accuracy of multimodal density is analyzed by means of the Monte Carlo method. It is shown that the soft clustering is more advantageous than the hard one. While a moderate increase in the number of clusters also increases the calculation time, it considerably reduces the estimation error.     

Comparison of analytical and semi-preparative columns for high-performance liquid chromatography-solid-phase extraction-nuclear magnetic resonance

The application of analytical and semi-preparative columns in reversed-phase liquid chromatography-solid-phase extraction-nuclear magnetic resonance (HPLC-SPE-NMR) was compared. The work was aiming at separating a higher sample amount in a single run and in this way to reduce the necessary NMR measurement time of separated compounds. Several parameters for compound separation and trapping procedures were optimised: flow rate of HPLC and make-up water pumps, choice of stationary phase cartridges and drying time. The separation and loadability of nine model compounds on analytical and semi-preparative columns was determined, as well as the focussing capacity of SH-type SPE cartridges. It was found that a semi-preparative column--or multiple peak trapping on analytical columns--gave better results than a standard 4.6mm analytical column for non-polar compounds (e.g. flavonoid aglycones, sesquiterpene lactones, non-polar terpenes, logP>2), but for polar compounds (logP<-2) did not offer any advantage over an analytical column, or was even disadvantageous. For intermediately polar compounds (-2相似文献   

Probabilities of high excursions of Gaussian fields

Let {ξ(t), t ∈ T} be a differentiable (in the mean-square sense) Gaussian random field with E ξ(t) ≡ 0, D ξ(t) ≡ 1, and continuous trajectories defined on the m-dimensional interval T ì \mathbbR^m T \subset {\mathbb{R}^m} . The paper is devoted to the problem of large excursions of the random field ξ. In particular, the asymptotic properties of the probability P = P{−v(t) < ξ(t) < u(t), t ∈ T}, when, for all t ∈ T, u(t), v(t) ⩾ χ, χ → ∞, are investigated. The work is a continuation of Rudzkis research started in [R. Rudzkis, Probabilities of large excursions of empirical processes and fields, Sov. Math., Dokl., 45(1):226–228, 1992]. It is shown that if the random field ξ satisfies certain smoothness and regularity conditions, then P = e^−Q  + Qo(1), where Q is a certain constructive functional depending on u, v, T, and the matrix function R(t) = cov(ξ′(t), ξ′(t)).     

Ethidium Binding to Salmonella enterica ser. Typhimurium Cells and Salmon Sperm DNA

Bacterial resistance to antibiotics due to increased efficiency of the efflux is a serious problem in clinics of infectious diseases. Knowledge of the factors affecting the activity of efflux pumps would help to find the solution. For this, fast and trustful methods for efflux analysis are needed. Here, we analyzed how the assay conditions affect the accumulation of efflux indicators ethidium (Et⁺) and tetraphenylphosphonium in Salmonella enterica ser. Typhimurium cells. An inhibitor phenylalanyl-arginyl-β-naphtylamide was applied to evaluate the input of RND family pumps into the total efflux. In parallel to spectrofluorimetric analysis, we used an electrochemical assessment of Et⁺ concentration. The results of our experiments indicated that Et⁺ fluorescence increases immediately after the penetration of this indicator into the cells. However, when cells bind a high amount of Et⁺, the intensity of the fluorescence reaches the saturation level and stops reacting to the accumulated amount of this indicator. For this reason, electrochemical measurements provide more trustful information about the efficiency of efflux when cells accumulate high amounts of Et⁺. Measurements of Et⁺ interaction with the purified DNA demonstrated that the affinity of this lipophilic cation to DNA depends on the medium composition. The capacity of DNA to bind Et⁺ considerably decreases in the presence of Mg²⁺, Polymyxin B or when DNA is incubated in high ionic strength media.     

Complex Scaling Method for Three- and Four-Body Scattering Above the Break-up Thresholds

A formalism based on the complex-scaling method is presented to solve the few particle scattering problem in configuration space using bound state techniques with trivial boundary conditions. Several applications to A = 3,4 systems are presented to demonstrate the efficiency of the method in computing elastic as well as break-up reactions with Hamiltonians including both short and long-range interaction.     

Antihydrogen formation via antiproton scattering on positronium between the $e^{-} +\bar {H}(n = 2)$ and e−+H̄(n=3)$e^{-}+\bar {H}(n = 3)$ thresholds

The charge exchange reaction \(\bar {\mathrm {p}} + \text {Ps} \rightarrow \mathrm {e}^{-} + \bar {\mathrm {H}} \), of interest for the future experiments (GBAR, AEGIS, ATRAP, ...) aiming to produce antihydrogen atoms, is investigated in the energy range between the \(\mathrm {e}^{-}+\bar {\mathrm {H}}(n = 2)\) and \(\mathrm {e}^{-}+\bar {\mathrm {H}}(n = 3)\) thresholds. An ab-initio method based on the solution of the Faddeev-Merkuriev equations is used. Special focus is put on the impact of the Feshbach resonances and the Gailitis-Damburg oscillations, appearing in the vicinity of the \(\bar {\mathrm {p}} +\text {Ps}(n = 2)\) threshold, on the \(\bar {\mathrm {H}}\) production cross section.