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用人脊髓灰质炎病毒分别感染Vero细胞和稳定表达p35基因的Vero35细胞,经细胞核DAPI染色,细胞DNA琼脂糖电泳和TDT生物素原位标记等方法证实病毒感染的细胞具有典型的凋亡细胞学特征和生物化学特征.实验结果表明人脊髓灰质炎病毒感染Vero细胞诱发的细胞死亡能被昆虫杆状病毒凋亡抑制基因p35抑制,为ICE类蛋白水解酶途径的细胞凋亡. 相似文献
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A novel metallo‐organically templated pentaborate with layered framework, [Cd(TETA)(C2H3O2)][B5O6(OH)4] ( 1 ) (TETA = triethylenetramine), was synthesized under mild solvothermal conditions. The structure was determined by single‐crystal X‐ray diffraction and further characterized by FT‐IR spectroscopy, elemental analysis, thermogravimetric analysis, and photoluminescence spectroscopy. The structure consists of an isolated polyborate anion [B5O6(OH)4]– and the cadmium complex cation of [Cd(TETA)(C2H3O2)]+, which contains both organic amine and organic acid ligands. The [B5O6(OH)4]– units are connected together by hydrogen bonds, and a 2D sheet‐like framework with rectangle‐like 12‐membered boron rings are formed. The [Cd(TETA)(C2H3O2)]+ complex cations are located in the free space between the layers and connect the adjacent borate layers through hydrogen bonds to form a three‐dimensional supramolecular network. The luminescent properties of the compound were studied for the first time in the series of metallo‐organically‐templated pentaborates, and a blue luminescence occurs with an emission maximum at 468 nm upon excitation at 397 nm. 相似文献
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Tao HU Haobo BAO Changgong MENG Jun ZHAO Yongxian YU Huilong WANG Shengyang TAO Shuqin LIU 《大学化学》2018,33(11):1-5
The development and integration of information technology with higher education facilitate the construction of the first-class online course, which has been a key objective for undergraduate education reform. Based on the student-centered idea, it is a valuable exploration and practice to integrate the online course with traditional teaching, and make efforts on innovative digitized teaching mode by taking the advantage of information technology. 相似文献
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可控相转变温度热敏高分子的制备及其在免疫分析中的应用 总被引:10,自引:0,他引:10
合成了一种新型的快速响应热敏高分子聚N-异丙基丙烯酰胺-丙烯酰胺[P(NIP-co-AA)],通过改变丙烯酰胺的含量可以改变高分子的临界溶解温度(LCST),使之用于不同用途。其中,将相转变温度(Ttr)在37℃的热敏高分子用于免疫分析的载体,建立了夹心型荧光免疫分析兔IgG的新方法。与聚N-异丙基丙烯酰胺(PNIP)作载体相比,两灵敏度相当,但由于相转变温度的提高,使得免疫反应的温度更接近于生物体的生理环境,并使免疫反应速率得到提高。该方法线性范围为0-1000μg/L;检出限为10μg/L。用于兔血清中兔IgG的测量,结果令人满意。 相似文献
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Yang Yang Jian Wu Yu Wang Jiang Zhu Ruibin Liu Prof. Dr. Changgong Meng 《无机化学与普通化学杂志》2011,637(1):137-141
A new protonated borophosphate (H3O)Mg(H2O)2[BP2O8]·H2O ( 1 ) was synthesized under mild hydrothermal conditions and characterized by single‐crystal X‐ray diffraction, FTIR spectroscopy and TG‐DTA. The compound crystallizes in the hexagonal system, space group P6(1)22 (No 178), a = 9.4462(7) Å, c = 15.759(2) Å, V = 1217.8(2) Å3, and Z = 6. There exist infinite helical $^1_\infty$ {[BP2O8]3–} ribbons built up from corner‐sharing PO4 and BO4 tetrahedra, which are connected by MgO4(H2O)2 leading to an infinite three‐dimensional open‐framework. The H3O+ ions are located at the free thread of the helical ribbons, whereas crystallized water occupy the channels of the helical ribbons. The dehydration of the compound occurs at a higher temperature which is presumably due to the anisotropic hydrogen bonds in the crystal structure. The luminescent properties of the compound were studied. 相似文献