Oral <Emphasis Type="Italic">L</Emphasis>-Carnitine Supplementation and Exercise Metabolism

Summary. Oral L-carnitine supplementation is frequently reported to have beneficial effects on exercise capacity in clinical populations and has been considered as a potential ergogenic aid for endurance athletes. However, this latter view is largely unsubstantiated possibly due to many experimental studies being poorly controlled or difficult to compare. The potential for oral L-carnitine supplementation to influence skeletal muscle carnitine content has been questioned and there are several key factors identified that may explain variations between study outcomes. Recent more well controlled research suggests some potential for L-carnitine to act as a key regulator of cellular stress, possibly through an impact on the integration of carbohydrate and lipid metabolism, and this work should be followed up in future by well controlled studies in both athlete and clinical subject groups.     

The (d, 6Li) reaction on 24Mg, 26Mg and 28Si at 35 MeV

Data for the (d, ⁶Li) reaction on targets of ²⁴Mg, ²⁶Mg and ²⁸Si have been obtained at 35 MeV bombarding energy. Angular distributions were measured for low-lying states in the residual nuclei. Zero-range distorted-wave Born approximation (DWBA) calculations have been used to analyze the data. The DWBA calculations account for the shapes of the experimental distributions reasonably well. The observation of significant population of unnatural parity states implies, however, that other transfer mechanisms may be important. The experimental spectroscopic factors are in qualitative agreement with those obtained from SU(3) theory.     

Non-invasive determination of ethanol, propylene glycol and water in a multi-component pharmaceutical oral liquid by direct measurement through amber plastic bottles using Fourier transform near-infrared spectroscopy

Fourier transform near-infrared (FT-NIR) spectroscopy was used to quantify rapidly the ethanol (34-49% v/v), propylene glycol (20-35% v/v) and water (11-20% m/m) contents within a multi-component pharmaceutical oral liquid by measurement directly through the amber plastic bottle packaging. Spectra were collected in the range 7302-12,000 cm-1 and calibration models set-up using partial least-squares regression (PLSR) and multiple linear regression. Reference values for the three components were measured using capillary gas chromatography (ethanol and propylene glycol) and Karl Fischer (water) assay procedures. The calibration and test sets consisted of production as well as laboratory batches that were made to extend the concentration ranges beyond the natural production variation. The PLSR models developed gave standard errors of prediction (SEP) of 1.1% v/v for ethanol, 0.9% v/v for propylene glycol and 0.3% m/m for water. For each component the calibration model was validated in terms of: linearity, repeatability, intermediate precision and robustness. All the methods produced statistically favourable outcomes. Ten production batches independent of the calibration and test sets were also challenged against the PLSR models, giving SEP values of 1.3% v/v (ethanol), 1.0% v/v (propylene glycol) and 0.2% m/m (water). NIR transmission spectroscopy allowed all three liquid constituents to be non-invasively measured in under 1 min.     

Using IMAP technology to identify kinase inhibitors: comparison with a substrate depletion approach and analysis of the nature of false positives

IMAP is a fluorescence polarisation-based assay method which can be applied to the measurement of protein kinase activity. Using a model serine/threonine kinase we found that IMAP generated a good assay window (Z' > 0.8), was very tolerant of DMSO, and was flexible with respect to sample processing (stopped reactions were stable over a period of several days). Using a set of six low molecular weight inhibitors of the kinase, we found a good correlation between IMAP and scintillation proximity assay (SPA) potency data. IMAP, which measures product accumulation, was compared in an HTS setting with a substrate depletion method (luminescence-based measurement of ATP concentration). There was a reasonable (approximately 50%) overlap in primary hits from a 17,000 compound set, but more apparent false positives were generated from the IMAP method. We followed up the compounds that showed activity in the IMAP method but not in the luminescence assay. Approximately 10% of these compounds displayed intrinsic fluorescence, suggesting that they were false actives by virtue of intrinsic spectroscopic properties. Compound activity by competition of phosphopeptide binding to IMAP beads can occur with high concentrations of chelating compounds, but did not occur with any of the false actives, suggesting that this form of interference is rare.     

Piecewise--planar representation of vowel formant frequencies

The first three formant frequencies for 778 steady-state tokens of 30 nonretroflex vowel types uttered by a female speaker are found to lie close to a piecewise-planar surface (expressed numerically as 0.634F1 +0.603F2 -- 0.485F3 -- 366 = 0, for F2 greater than 0.027F1 +1692 and 0.686F1 -- 0.528F2 -- 0.501F3 +1569 = 0, otherwise). The rms distance of the vowels from this surface is only 86 Hz. The intersection between the two planes is a line of nearly constant F2, corresponding closely to the F2 of a uniform vocal tract of the same length as our speaker's. The piecewise-planar representation also suggests a way to test the hypotheses of uniform and nonuniform formant-frequency scaling between speakers.     

基于垂直谐振腔结构的半导体光放大器理论分析

由新型垂直腔半导体光放大器（VCSOAs）器件结构出发,在反射模式和透射模式两种 情况下,计算了VCSOAs的分布布拉格反射堆（DBR）反射率、单程增益与增益、带宽的关系 ,其中DBR反射率的增大伴随着增益的上升,但也伴随着带宽的减小.通过引入增益-带宽 积的概念,以均衡增益与带宽.计算中表明:10 dB以上增益,50 GHz以上带宽可以实现. 计算结果与文献实验相吻合,对优化VCSOAs的结构设计和性能有所裨益.     

Optimisation of partial least squares regression calibration models in near-infrared spectroscopy: a novel algorithm for wavelength selection

A novel optimisation algorithm is presented for full spectrum calibration models in near-infrared (NIR) spectroscopy. The algorithm is used to investigate the affect of removing continuous spectral regions on parameters critical to the validity of the model (e.g. explained variance, bias etc.) and ultimately identify and remove problem areas of the spectrum. As an example of its application, this paper shows how to optimise partial least squares regression (PLSR) calibration models for predicting moisture content within an intact pharmaceutical product and how problems due to changes in the nature of samples since setting up the original model may be eliminated. On application of two validated calibration models to a new set of samples unacceptable results were obtained for bias (-0.26 and -0.21% m/m moisture content) between the NIR predicted values and the true values (Karl Fischer analysis). The optimisation algorithm identified small regions of the spectrum, which if included in development of the models contributed significant bias to the final prediction. On removal of these problem regions the calibration models were found to be equally accurate and precise, but with the added advantage of robustness to a variable region of the sample spectrum (bias reduced to -0.05 and -0.09% m/m).