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Arulraj Ruba A. Gajjela Raju Chauthe Siddheshwar Kisan Bagadi Muralidhararao Mathur Arvind 《Chromatographia》2022,85(6):497-506
Chromatographia - In drug discovery environment, determination of residual solvents is indispensable and requires rapid analysis covering a maximum number of commonly used solvents especially with... 相似文献
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Syed Dilshad Alam Mirza Adil Beg Muralidhararao Bagadi Marcello Locatelli Zeid A. ALOthman Mohd Mustaqeem Imran Ali 《Journal of separation science》2023,46(21):2300582
The extraction of berberine was carried out from Berberis vulgaris, Berberis aquifolium, and Hydrastis canadensis plants using ethanol and water (70:30, v/v). The extracted berberine was characterized by ultraviolet-visible and Fourier-transform infrared spectroscopy. The purity of berberine was ascertained by thin-layer chromatography using n-propanol-formic acid-water (95:1:4) and (90:1:9) solvents. hRf values were in the range of 44–49 with compact spots (diameter 0.2–0.4 cm). HPLC was carried out using ammonium acetate buffer and acetonitrile in gradient mode with Zodiac (4.6 × 150 mm, 3 μm) column. The flow rate was 1.0 mL/min and detection was at 220 nm. The values of separation and resolution factors of the standard and the extracted berberine were in the range of 1.13–1.16 and 1.40–1.71, respectively. A comparison has shown that both thin-layer chromatography and high-performance liquid chromatography (HPLC) methods found applications in different situations and requirements. The extracted berberine samples were used to treat Leishmaniosis and the results showed better activity of berberine in comparison to the standard drug Amphotericin B. Briefly, the reported research is a novel and may be used to extract berberine from plants, separation and identification of berberine by thin layer chromatography and HPLC and to treat Leishmaniosis. 相似文献
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