气相色谱-质谱法测定富硒酵母中的硒蛋氨酸

建立了气相色谱-质谱（GC-MS）测定富硒酵母中硒蛋氨酸含量的方法。比较了3种从样品中提取硒蛋氨酸方法的效果。样品在三羟甲基氨基甲烷（Tris）缓冲液中酶解24 h后,以丁醇及三氟乙酸酐为衍生化试剂对硒蛋氨酸进行衍生化,采用选择离子模式对衍生物进行GC-MS测定。硒蛋氨酸的回收率为98.5%~103.7%,相对标准偏差为0.9%~2.4%,检出限为0.5 mg/L（S/N=3）。对实际样品进行测定,得到样品中硒蛋氨酸的含量结果。该法简便快速,准确可靠,灵敏度高。     

氢化物发生-原子荧光光谱法测定富硒酵母中的有机硒和无机硒

建立了氢化物发生-原子荧光光谱法测定富硒酵母中硒的分析方法。研究了仪器的工作条件、试剂对硒测定的影响;探讨了消化试剂对样品消化的影响和共存离子的干扰及消除。在优化的工作条件下,硒的检出限为0.078μg/L,相对标准偏差为0.45%,线性范围为0~100μg/L。应用该方法检测了不同富硒酵母中的总硒含量为1.40~3.20 mg/g;其中,有机硒含量为1.30~2.97 mg/g,加标回收率为95.4%~104.3%。     

Full distributed fiber optical sensor for intrusion detection in application to buried pipelines

Based on the microbend effect of optical fiber, a distributed sensor for real-time continuous monitoring of intrusion in application to buried pipelines is proposed. The sensing element is a long cable with a special structure made up of an elastic polymer wire, an optical fiber, and a metal wire. The damage point is located with an embedded optical time domain reflectometry (OTDR) instrument. The intrusion types can be indicated by the amplitude of output voltage. Experimental results show that the detection system can alarm adequately under abnormal load and can locate the intrusion point within 22.4 m for distance of 3.023 km.     

一类具有病毒变异的Logistic死亡率SEIR传染病模型研究分析

本文建立了一类具有病毒变异的Logistic死亡率SEIR传染病模型,借助Lyapunov函数和LaSalle''s不变原理,证明了无病平衡点全局稳定性.利用代数方法构造Lyapunov函数,证明了地方病平衡点全局稳定性.另外,通过数值模拟分析了参数对疾病传播的影响.     

一类具有脉冲接种、双时滞的SEIRS传染病模型研究分析（英文）

In this paper, an SEIRS epidemic model with pulse vaccination and two time delays is proposed. By using stroboscopic map and comparison principle, the disease-free periodic solution(DFPS for short) is obtained and the global asymptotic stability of the DFPS is proved. The sufficient conditions for the permanence of the model are obtained. In addition, numerical simulations are done to confirm our theoretical results.