三种1,10-邻菲咯啉-铜(Ⅱ)-L-氨基酸配合物与DNA的相互作用

The Interaction of three complexes [Cu(phen)(Gly)(H₂O)]·ClO₄·2.5H₂O (1), [Cu(phen)(L-Val)(H₂O)]·ClO₄ (2) and [Cu(phen)(L-Ile)(H₂O)]·ClO₄ (3) (phen=1,10-phenanthroline, Gly=glycinate, L-Val=L-valinate, L-Ile=L-isoleucinate) with DNA have been investigated by electronic absorption spectroscopy, ethidium bromide(EB) fluorescence spectroscopy, viscosity and gel electrophoresis measurements. The intensity of maximal absorption peaks from absorption spectra are weakened with dropping DNA into the complexes. The emission intensity of EB-DNA systems in fluorescence spectroscopy have descended 50%, when the data of C_Cu / C_DNA is 30～40. Moreover, the viscosity of DNA increase and then decrease with the increasing of complexes. All of the results indicate that the interaction of complexes with DNA are partial intercalation, and the result of agarose gel electrophoresis show that the complexes can cleave pBR322 DNA in the present of H₂O₂ / vitamin C.     

蛋白质界面取向的实验控制与表征

在生物材料、生物传感器、生物燃料电池等应用中都涉及到蛋白质界面吸附,而其中一个关键的科学问题就是蛋白质在基底界面上的吸附取向,控制好正确的取向是发挥蛋白质生物功能的关键。本文归纳和比较了各种用于控制蛋白质界面取向的固定化方法,总结了影响蛋白质界面吸附取向的因素,并对当前应用较多的表征蛋白质在界面吸附取向的方法进行了综述,最后指出蛋白质取向控制的机理研究将有助于推动相关应用领域的迅速发展。