TRANSFER OF THE ATRAZINE-RESISTANT GENE OF BLACK NIGHTSHADE TO SOYBEAN CHLOROPLAST GENOME AND ITS EXPRESSION IN TRANSGENIC PLANTS

The atrazine-resistant psbA gene of black nightshade was transferred to the chloroplast genome of atrazine-susceptible soybean by means of ovary microinjection during the stage of zygote. The identification was carried out by using the methods of spraying the leaves directly with atrazine solution, examining the change of leaf fluorescence kinetics under a brighter light induction, molecular hybridization, etc. The experimental results show that the transgenic soybean plants do have been obtained for the first time.     

龙葵Atrazine抗性基因向大豆叶绿体的转移及在转基因植株中的表达

本文将龙葵Atrazine抗性psbA基因采用合子期子房微注射法,导入对Atrazine敏感的大豆叶绿体基因组中,经直接用Atrazine涂抹叶片及叶片在较强光诱导下荧光动力学研究和间接分子杂交试验等检测方法鉴定,表明本项研究首次获得了转基因大豆植株。