MicroRNAs电化学生物传感器在乳腺癌检测中的研究进展

MicroRNAs是一类内源性非编码小RNA分子,可调控靶基因的表达。特异性microRNAs的失调在诸如癌症、心血管疾病、免疫疾病、神经退行性疾病和皮肤疾病等的发展过程中起着关键作用,常作为疾病早期诊断和预后的生物标志物。电化学生物传感器由于其灵敏、快速、成本低等优势,已经成为传统microRNAs检测方法的一种很有前途的替代方法。本文综述了基于microRNA电化学生物传感器检测乳腺癌的研究进展,并对其所构建的电化学生物传感器的检测方法进行了分析探讨。     

Determination of Pb（II） in environmental water by nano-ceria-graphene modified electrodeEI北大核心CSCD

Nafion solution as a binder,the Gr/CeO2 /Nafion/GCE modified electrode was prepared based on graphene （Gr）-nano cerium oxide （CeO2 ）composite materials. The electrochemical behavior of Pb（II）on the modified electrode was investigated using differential pulse anodic stripping voltammetry （DPASV）. Subsequently,a determination method for Pb（II）was established. The concentrations and ratio of composite materials,buffer solution pH,modification amount,enrichment potential and time were optimized. Under the optimal conditions ,the peak current of Gr/CeO2 /Nafion/GCE showed good linearity in the range of 0.24-48.31 μµmol/L of Pb（II）,and the detection limit was 4.3 nmol/L. Interference experiments showed that the other common anions and cations in water samples caused little interference to Pb（II）detection. Furthermore,the interference of Cu（II）within 10 times is acceptable. The modified electrode was used to the detection of Pb（II） in four complex water samples,and the recovery rates of the spiked assays were from 89.5% to 107.7%. © 2023, Youke Publishing Co.,Ltd. All rights reserved.