EXPRESSION AND SECRETION OF preS CONTAINING HEPATITIS B SURFACE ANTIGEN IN VACCINIA VIRUS SYSTEM

The expression and secretion of preS containing hepatitis B surface antigen in vaccinia virus system was investigated. The human TK~- 143 cells were infected with the recombinant vaccinia viruses vTMS-1 or vTLS-1. Cells infected with vTMS-1, which contains the preS2+S gene, produced preS2 containing middle HBsAg proteins. Similarly, cells produced preS1 containing large HBsAg proteins upon infection with vTLS-1, which carries the preS1+preS2+S gene. The expression products could be secreted and form 22 nm particles. They reacted specifically with anti-preS1 and/or anti-preS2 monoclonal antibodies, and exhibited pHSA-receptor (for polymerized human serum albumin) activity. In addition, the major S components of hepatitis B surface antigen were also present in the products expressed by vTMS-1 and vTLS-1.     

乙型肝炎病毒表面抗原preS基因在痘苗病毒系统中的分泌型表达

本文对带preS2的乙肝病毒表面抗原基因以及带preS1和preS2的乙肝表面抗原全基因的重组痘苗病毒vTMS-1和vTLS-1的表达和分泌进行了研究。在人TK-143细胞中它们能表达各自所编码的蛋白——乙肝表面抗原中分子蛋白和大分子蛋白。产物是可分泌的,并能形成22nm颗粒。分泌在培液中的表达产物能分别与抗preS2和抗preS1的单抗反应,并保持了天然表面抗原大分子和中分子蛋白所具有的多聚人血清白蛋白的受体活力。它们的表达产物中都还合有乙肝表面抗原主蛋白的成分。