| EXPRESSION AND SECRETION OF preS CONTAINING HEPATITIS B SURFACE ANTIGEN IN VACCINIA VIRUS SYSTEM |
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| 作者姓名： | 汪垣 王少芹 冯宗铭 钟武威 孔玉英 李载平 陆选永 姚光弼 |
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| 作者单位： | Shanghai Institute of Biochemistry，Academia Sinica，Shanghai 200031，PRC，Shanghai Institute of Biochemistry，Academia Sinica，Shanghai 200031，PRC，Shanghai Institute of Biochemistry，Academia Sinica，Shanghai 200031，PRC，Shanghai Institute of Biochemistry，Academia Sinica，Shanghai 200031，PRC，Shanghai Institute of Biochemistry，Academia Sinica，Shanghai 200031，PRC，Shanghai Institute of Biochemistry，Academia Sinica，Shanghai 200031，PRC，Jing'an Central District Hospital，Shanghai，PRC，Jing'an Central District Hospital，Shanghai，PRC |
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| 摘 要： | The expression and secretion of preS containing hepatitis B surface antigen in vaccinia virus system was investigated. The human TK~- 143 cells were infected with the recombinant vaccinia viruses vTMS-1 or vTLS-1. Cells infected with vTMS-1, which contains the preS2+S gene, produced preS2 containing middle HBsAg proteins. Similarly, cells produced preS1 containing large HBsAg proteins upon infection with vTLS-1, which carries the preS1+preS2+S gene. The expression products could be secreted and form 22 nm particles. They reacted specifically with anti-preS1 and/or anti-preS2 monoclonal antibodies, and exhibited pHSA-receptor (for polymerized human serum albumin) activity. In addition, the major S components of hepatitis B surface antigen were also present in the products expressed by vTMS-1 and vTLS-1.
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EXPRESSION AND SECRETION OF preS CONTAINING HEPATITIS B SURFACE ANTIGEN IN VACCINIA VIRUS SYSTEM |
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| WANG YUAN WANG SHAO-QIN FENG ZONG-MING ZHONG WU-WEI KONG YU-YING LI ZAI-PING.EXPRESSION AND SECRETION OF preS CONTAINING HEPATITIS B SURFACE ANTIGEN IN VACCINIA VIRUS SYSTEM[J].Science in China(Chemistry),1990(9). |
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| Authors: | WANG YUAN WANG SHAO-QIN FENG ZONG-MING ZHONG WU-WEI KONG YU-YING LI ZAI-PING |
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| Abstract: | The expression and secretion of preS containing hepatitis B surface antigen in vaccinia virus system was investigated. The human TK~- 143 cells were infected with the recombinant vaccinia viruses vTMS-1 or vTLS-1. Cells infected with vTMS-1, which contains the preS2+S gene, produced preS2 containing middle HBsAg proteins. Similarly, cells produced preS1 containing large HBsAg proteins upon infection with vTLS-1, which carries the preS1+preS2+S gene. The expression products could be secreted and form 22 nm particles. They reacted specifically with anti-preS1 and/or anti-preS2 monoclonal antibodies, and exhibited pHSA-receptor (for polymerized human serum albumin) activity. In addition, the major S components of hepatitis B surface antigen were also present in the products expressed by vTMS-1 and vTLS-1. |
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| Keywords: | hepatitis B virus vaccinia virus in vivo reeombination HBsAg middle large proteins secretory expression |
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