同时测定三辛胺和三辛胺盐酸盐的双波长分光光度法

提出了利用双波长分光光度法测定三辛胺（ＴＯＡ）及三辛胺盐酸盐（ＴＯＡ·ＨＣｌ）的一种新方法，即ＴＯＡ和ＴＯＡ·ＨＣｌ在ＣＣｌ４溶液中与Ｉ２生成电荷转移化合物，其最大吸收波长分别为２９３ｎｍ和２６３ｎｍ，两组分同时存在时可利用吸光度的加和性得联立方程，分别求出两组分的含量。ＴＯＡ含量在０～３５ｍｇ／Ｌ，ＴＯＡ·ＨＣｌ含量在０～３１ｍｇ／Ｌ范围内符合比耳定律。该法简便快速，方法的回收率为９５．５％～１０４．６％，能够满足分析的需要     

利用碘—三辛胺盐酸盐反应分光光度测定TOA,HCl

研究了测定三辛胺盐酸盐的一种新方法－碘光谱法。ＴＯＡ，ＨＣｌ在四氯化碳溶液中与生成电荷转移化合物，该物质的最大吸收波长为３７０ｎｍ，ＴＯＡ，ＨＣｌ在０－４×１０＾－４ｍｏｌ．Ｌ范围内符合比耳定律。该方法准确，简便，快速，其它溶剂的干扰较小，回收率在９４．５％－１０１．９％，能够满足分析的需要。     

野生大豆(Glycine soja SH1)球蛋白glycinin Gy4基因家族的两种表达拷贝

本文研究我国野生大豆(Glycine soja)种子贮藏蛋白基因的结构,并与栽培大豆进行比较,构建成野生大豆Glycine soja SH1和栽培大豆Glycine max子叶cDNA库。用已知含球蛋白glycinin Gy 4基因的克隆DNA λS 312为探针,从两个cDNA库中分离出6个克隆。其中两个克隆pWS 228与pWS 242含全长cDNA,克隆cDNA的限制酶图谱和cDNA与基因组DNA的Southern法杂交表明它们代表野生大豆球蛋白glycinin Gy 4基因家族的两种表达拷贝。pWS 228 cDNA的部分序列已测定并与栽培大豆相应顺序作比较。分子杂交还证明Ⅱ类glycinin基因家族的组编在野生大豆胚形成过程中的变动。     

硫酸铝生产过程中的萃取法除铁

Fe;萃取;硫酸铝生产过程中的萃取法除铁     

胶质液态泡沫的萃取性能

制备了包含萃取剂的胶质液态泡沫（ＣＬＡ）,测定了包含Ｎ１９２３或ＴＢＰ的ＣＬＡ萃取醋酸的性能,以及包含Ｐ５０７的ＣＬＡ萃取稀土离子时稀土在ＣＬＡ水质滑腻壳层中的渗透系数．结果表明将萃取剂包含于ＣＬＡ中,对其粒径、粘度及稳定性等不产生明显影响,但对萃取分配及传质十分有利．     

TWO EXPRESSED COPIES OF GLYCININ Gy4 GENE SUBFAMILY IN WILD SOYBEAN Glycine soja SHI

To study the structure of genes encoding seed storage proteins of the wild variety of soybean (Glycine soja) in comparison with those of the cultivated (Glycine max),cDNA libraries from cotyledon poly(A)~+ RNA of both wild and cultivated soybeans were constructed.Six clones were isolated using the genomic clone λS312 which contains the coding sequence for the glycinin subunit precursor (A_5A_4B_3) as probe for molecular hybridization. Two clones pWS228 and pWS242 contain the full length of cDNA.Restriction mapping of the cloned cDNA and Southern hybridization of the cDNA with genomic DNA showed two expressed copies of glycinin Gy4 gene subfamily in wild soybean Glycine soja SHI. Alteration of DNA arrangement in group-Ⅱ glycinin gene subfamily during embryogenesis was also demonstrated.     

氯化炭黑中氯的分析方法

提出了改性炭黑－氯化碳黑的分析方法,即样品在管式炉中通氧灰化,使结合在炭黑有机官能团上的氯转化为可测定的无机氯,用吸收液吸收后,汞量法测定氯含量,方法简便快速,准确度高,回收率在９６．８％－１０１．７％,精密度好,相对标准偏差为１．１７％,不需用特殊仪器,适合工业生产中的监控分析。     

同时测定三辛妥和三辛胺盐酸盐的双波长分光光度法

提出了利用双波长分光光度法测定三辛胺及三胺盐酸盐的一种新方法,即ＴＯＡ和ＴＯＡ．ＨＣｌ在ＣＣｌ４溶液中与Ｉ２生成电荷转移化合物,其最大吸收波长分别为２９３ｎｍ和２６３ｎｍ,两组分同时存在时可利用吸光度和加和性得联立方程分别求出两组分的含量。     

大豆(Glycine max)贮藏蛋白两种编码顺序的克隆与性质

本文证明大豆(Glycine max)贮藏蛋白的两种编码顺序已经克隆。并通过分子杂交和体外翻译研究了它们的性质。其中一顺序与两种分子量不同的mRNA杂交,杂交选择mRNA在体外翻译成60kd和42kd多肽,推测为7S贮藏蛋白的α和β亚基,另一顺序则与分子量为0.7×10~6dalton的mRNA杂交,杂交选择mRNA在体外翻译成57kd和49kd多肽。     

CLONING AND CHARACTERIZATION OF TWO CODING SEQUENCES FOR STORAGE PROTEIN IN SOYBEAN Glycine max

Two coding sequences for storage protein in Glycine max have been cloned. They were characterized by molecular hybridization and in vitro translation. One sequence happens to hybridize to two sized mRNAs which are translated in vitro to 60 Kd and 42 Kd polypeptides corresponding to a and β subunits of 7S storage protein, and the other is hybridizable with 0.7×10~6 dalton mRNA giving rise to 57 Kd and 49 Kd polypeptides in vitro translation.