第二类窃密信道中的组合数学方法

当信息在第二类窃密信道中传输时,线性码的广义汉明重量谱完全描述的它在该信道中的密码学特征.计算一个线性码的广义汉明重量谱是一个基本问题,首先提出了线性码的“最简基”的概念.在此基础上给出了一般线性码子码的几种计数公式,并给出了它们之间等价性的证明.     

应用农杆菌Ti质粒系统将外源基因转入籼稻细胞研究

本文证明经复合酚类化合物预处理的菌株与籼稻培养细胞在普通培养液。特别是在复合诱导培养液(培养过番茄下胚轴切段、胡萝卜细胞及农杆菌的培养滤液)中共培养时,均可发现有较多的细菌附着于水稻细胞表面,并且菌体周围有纤维丝的形成。在复合处理组中,位于pGV3850::1103neo嵌合质粒T-DNA上的NPT Ⅱ基因及NOS基因在籼稻培养细胞中获得了转移与表达。Southern blot分析证明了外源基因整合到受体细胞的基因组中。     

Integration and Expression of Human Growth Hormone Gene in Caladium bicolor

Human growth hormone (hGH) gene has been inserted into the plasmid pLGV1103 to give the recombinant plasmid pLB-9. It has been introduced into the agrobacterium containing plasmid pGV3850. The recombinant Ti plasmid pGL198(hGH) has been obtained by homologous recombination. The monocotyledon Caladium bicolor has been transferred with pGL198 (hGH) with the leaf-disk co-cultivation method, and transgenic plants have been regenerated. The results of nopaline analysis, NPT II detection Southern blot and Western blot show that the hGH gene was integrated into the genome of Caladium bicolor, and a 22-kD protein was synthesized in the transgenic plants.     

人生长激素基因在花叶芋中的整合与表达

本试验将人生长激素(hGH)基因插入质粒载体pLGV1103中,构建了重组质粒pLB-9,将此重组质粒引入携带Ti质粒pGV3850的农杆菌,经同源重组,获得共整合重组体pGL198(hGH)Ti质粒。用叶盘共培养法,使pGL198(hGH)转化单子叶植物花叶芋(Caladium bicolor),获得转基因再生植株。经胭脂碱、新霉素磷酸转移酶、Southern印迹以及Western印迹等分析,表明hGH基因已整合到花叶芋DNA中,并且合成了分子量为22kD的表达产物。     

诱导根癌农杆菌vir区基因表达水稻代谢物的分离,鉴定和生物活性研究

从孕穗期至开花期水稻(oryza sative L. cv. lndia IR 72)的茎叶提取液中, 筛选并分离出二种能高效诱导根癌农杆菌(Agrobacterium tumfaciens) vir 区基因表达的信号分子,这两种信号分子的化学结构分别是5,7,4'-三羟基-3'-5'-二甲氧基黄酮和5,4'-二羟基-3',5'-二甲氧基-7-β-D-葡萄糖氧基黄酮.     

应用电注射法将外源基因导入水稻种胚及获得转基因水稻植株研究

用我们实验室自制的电容放电式电激仪,成功地把质粒pLGVneo2103上的NPTⅡ基因导入两个水稻品种(籼稻品种三二矮和粳稻品种农虎6号)的种子胚细胞中,在含有100μg/ml Km的MS培养基上选择出抗性愈伤组织,并由此通过体胚发生途径再生出转基因植株,NPTⅡ检测和DNA分子杂交证实外源基因已在上述转化体中得以稳定的整合和表达.     

抗稻瘟病水稻品种叶片中脲类衍生物的分离鉴定和生物活性研究

从抗稻瘟病水稻品种(oryzae cav.)叶片中分离得到两种天然的脲类衍生物: 乙基-4-(邻硝基苯基)-3-硫代脲酸酯(1)和乙基-4-(邻硝基苯基)-3-脲酸酯. 它们的波谱分析数据已经测定. 生物活性研究表明化合物1具有特殊的生物活性 .     

INTRODUCTION OF FOREIGN GENES INTO THE SEED EMBRYO CELLS OF RICE BY ELECTRO-INJECTION AND THE REGENERATION OF TRANSGENIC RICE PLANTS

The NPTII gene has been successfully transferred to the seed embryo cells of two rice varieties (Oryza sativa L. subsp. indica cv. Sanerai and Qryza sativa L. subsp. japonica cv. Nonghu No. 6) by means of electroinjection. Resistant calli were screened out on MS medium with 100 μg/ml Km. Transgenic rice plants were regenerated via somatic embryogenesis. Both NPTII detection and Southern blot hybridization demonstrate that the foreign gene has integrated and expressed stably in the transformants.     

STUDIES ON INTRODUCTION OF FOREIGN GENES INTO CULTURED CELLS OF Oryza sativa INDICA USINC Agrobacterium Ti PLASMID SYSTEM

Either bacterial attachment or cellulose fibrillar elaboration was hardly observedduring the cocultivation of the cultured suspension cells of Oryza sativa Indica with thestrain C58C1 Rif~r of Agrobacterium tumefaciens that was not specially pretreated. Onthe other hand, quite a lot of Agrobacterium cells were found to adhere to the surface ofcultured rice cells and a number of cellulose fibrils were produced around the specifiedbacteria when phenolics-pretreated bacteria were cocultivated with rice suspension cellsin common culture media, especially in complex culture solutions. The complex culturesolution was the bacterium-free filtrate of hormone-containing MS medium which hadbeen utilized to incubate carrot cells and the newly wounded hypocotyl segments fromtomato and Agrobacterium cells. Detecting experiments demonstrated that both NPT Ⅱ andNOS genes, located on the T-DNA segment of chimaeric plasmid pGV3850 :: 1103neo, weretransferred and expressed in the cultured cells of O. sativa Indica in the