UPLC-QTOF-MS法研究甘油三酯作为水生环境中农药污染的潜在生物标志物

以鲫鱼为代表性研究对象, 采用超高效液相色谱-四极杆飞行时间质谱(UPLC-QTOF-MS)技术, 分别研究了3种农药(敌百虫、 高效氯氰菊酯和吡虫啉)对其体内代谢的影响. 通过Masslynx软件在MS ^E模式下采集数据, 使用UNIFI软件进行自动检测和数据过滤, 并将差异性物质与在线代谢组数据库进行比较, 鉴定出甘油三酯类(TGs)物质具有明显差异. 其中, 吡虫啉对TGs代谢的影响最大, 且肝脏的代谢差异比大脑更显著, 低浓度的急性暴露诱导肝脏中的TGs积累, 其含量与暴露浓度之间的关系符合Michaelis-Menten方程的增长趋势. 结果表明, TGs可在短时间内(≤2 h)灵敏地反映农药吡虫啉的低浓度(≤20 ng/mL)暴露, 可作为考察吡虫啉对水生生物毒性的潜在生物标记物, 有助于建立一种快速、 灵敏的预警方法.     

Effects of trichlorfon on the contents of acetylcholine and choline in fish brain microdialysis samples by UPLC-MS/MS methodEI北大核心CSCD

A combination of microdialysis sampling with ultra-high performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS）was used to quantitatively analyze the effects of organophosphorus pesticides trichlorfon on acetylcholine （ACh） and choline （Ch） in the brain of crucian carp. The mechanism of organophosphorus pesticides poisoning was preliminarily discussed,showing that the ACh content in the brain of fish increased significantly with the increase of the exposure time to trichlorfon,while the Ch content decreased. It indicated that organophosphorus pesticides inhibited the activity of cholinesterase in organisms. © 2023, Youke Publishing Co.,Ltd. All rights reserved.