君子兰花粉散粉前后合成的核糖核酸和蛋白质的作用

本文证明君子兰花粉在RNA和蛋白质合成完全被抑制的条件下仍可达到95％右左的萌发率,并长出有限长度的花粉管。RNA酶和蛋白酶均可影响直至完全阻止花粉的萌发和/或花粉管的伸长。统计表明:成熟花粉内已有的蛋白质可维持花粉管约70μm的伸长,预存mRNA转译的蛋白质可维持花粉管约530μm的伸长。散粉后合成的RNA和蛋白质对花粉管后继伸长的速度和晚期生长(>2400μm)都是必需的。环己亚胺3h或放线菌素D,10h以上的处理会引起花粉管畸形,畸变率与抑制剂浓度及处理时间相关。适量的抑制剂在培养的头5h内可阻碍生殖细胞通过分裂中期,10h以后加抑制剂,不再影响精细胞的形成。     

THE ROLE OF RNA AND PROTEIN SYNTHESIZED BEFORE AND AFTER DEHISCENCE OF THE ANTHER IN THE DEVELOPMENT OF POLLEN GRAINS OF Clivia nobilis

Under the condition of completely inhibited RNA and protein synthesis, the rate of pollen grain germination of Clivia nobilis still reaches about 95% and the pollen tubes grow up to a certain length.RNase or pronase E added to the medium affected the germination and the elongation of the pollen tubes till they were completely blocked. Statistical results show that the original proteins may maintain the elongation of pollen tubes at about 70 μm, the proteins translated from pre-existing mRNA maintain them at about 530 μm.The RNA and protein synthesized after dehiscence of the anther are necessary for the normal rate of subsequent elongation and the growth of pollen tubes in the later stage (>2400 μm). Treatment of long duration such as CHM 3h and AmD 10h gives rise to aberration of pollen tubes, the rate of aberration being related to the concentration of inhibitors and the duration of treatment.In the first 5h of culture a suitable amount of inhibitors may block the progress of meta- phase of the gen