Resonance-enhanced multiphoton ionization mass spectrometry using a two-color laser beam generated by stimulated Raman scattering.

Two-color (1+1') resonance-enhanced multiphoton ionization/time-of-flight mass spectrometry (REMPI/TOF-MS) combined with supersonic jet (SSJ) spectrometry has been demonstrated. The methodology uses a two-color laser beam consisting of a Stokes beam generated by stimulated Raman scattering (SRS) and a fundamental beam for the excitation and subsequent ionization of p-dichlorobenzene. The MPI signal was found to be substantially increased when a two-color laser beam was employed. This approach greatly simplifies the analytical system and reduces the cost of the instrumentation required for two-color ionization. The potential advantage of applying this method for the analysis of polychlorinated dibenzo-p-dioxin (PCDD) and polychlorinated dibenzofuran (PCDF) emitted from an incinerator is discussed.     

Synthesis of 2-amino-2,4-anhydro-3-O-(2-tetrahydropyranyl)-psicofuranose: a versatile intermediate for S-type locked nucleosides

2-Amino-2,4-anhydro-psicofuranose derivative was synthesized starting from d-fructose.     

Total synthesis of tylosin

Tylosin has been synthesized byregio- and stereoselective introduction of the amino disaccharide moiety and D-mycinose onto the previously synthesized 16-membered-ring aglycone.     

Stark effects on the phosphorescence spectra of p-benzoquinone

Stark effects on the phosphorescence spectra of p-benzoquinone have been studied in naphthalene crystals at temperatures between 1.7 and 30 K. The experimental data establish that the band splitting of 24 cm^?1 arises from a double minimum potential in the T₁ state.     

Ruthenium-catalyzed carbonylative cycloaddition of alpha-keto lactones with alkenes or alkynes: the participation of an ester-carbonyl group in cycloaddition reactions as the two-atom assembling unit

The reaction of benzofuran-2,3-dione derivatives 1 with CO and alkenes (or alkynes) results in a carbonylative [2+2+1] cycloaddition in which the ester-carbonyl group is incorporated into a two-atom assembling unit to give spirolactone derivatives 2. This reaction provides the first example of an ester-carbonyl group participating in a carbonylative cycloaddition reaction.     

Optimization of in-gel protein digestion system in combination with thin-gel separation and negative staining in 96-well plate format

Improvement of in-gel digestion efficiency is highly desirable for one- or two-dimensional gel electrophoretic separation and mass spectrometric (MS) analysis in proteomics, because the resultant increases in sequence coverage and MS signal intensity lead to higher confidence in protein identification. Here an optimized in-gel digestion system, in combination with thin-gel separation and negative staining in a high-throughput format using 96-well plates, is described. The combination of negative staining and protein separation on a 0.9 mm thick gel showed a clear improvement in in-gel digestion efficiency in comparison with the more typical protocols such as the combination of silver staining and a 1.0 mm gel. In addition, the use of 96-well plates to increase throughput did not decrease the efficiency of this strategy when the stirring of the gel pieces in processes such as destaining, washing, gel-shrinking and peptide extraction was performed by sonication instead of shaking the plates. This procedure was optimized and applied to identify proteins of the postsynaptic density fraction; 105 proteins were identified after SDS-PAGE separation.     

CHOICE OF COATINGS FOR THE OPTICAL ELEMENTS IN THE IRRADIATION SYSTEM OF VACUUM-ULTRAVIOLET RADIATION ABOVE 50 nm

The spectral throughput of a vacuum-ultraviolet irradiation system at the SOR-RING facility was examined with various combinations of aluminum-and gold-coated optical elements in a 2.2-m modified Wadsworth monochromator. We found that the optimum was a combination of an aluminum-coated collimating mirror, concave grating, and plane deflecting mirror, and a combination of a gold-coated collimating mirror, concave grating and an aluminum-coated plane deflecting mirror in the wavelength regions 190-110 nm and 110-50 nm, respectively.     

Large-scale separation of metallic and semiconducting single-walled carbon nanotubes

In the applications of single-walled carbon nanotubes (SWNTs), it is extremely important to separate semiconducting and metallic SWNTs. Although several methods have been reported for the separation, only low yields have been achieved at great expense. We show a separation method involving a dispersion-centrifugation process in a tetrahydrofuran solution of amine, which makes metallic SWNTs highly concentrated to 87% in a simple way.     

Force in scf theories

A new force approach is reported in which we improve the wavefunction so that the Hellmann-Feynman theorem is satisfied. A sufficient condition for the Hellmann-Feynman theorem to be satisfied is that the basis set includes AO derivatives ?x_r/?x_rfor any basis x_r. Here we test a procedure in which only the first derivative AOs are added to the “parent” AOs. The results are very encouraging.     

Synthesis of 2′-deoxyguanosine containing a N2-polyamine

The synthesis of benzylated N²-(4,7,10,13-tetraazatridec-1-yl)-2′-deoxyguanosines 4 was accomplished by a key nucleophilic reaction of the novel unsymmetrical polyamine 2 , with 3′,5′-O-(tetraisopropyldisiloxane-1,3-diyl)-2-chloro-2′-deoxyinosine ( 1 ).