Molecularly Imprinted Polypyrrole for DNA Determination

In this study graphite electrodes modified by a thin DNA‐imprinted polypyrrole layer, which was able to bind specific target‐DNA, are reported. For this aim, electrochemical synthesis of polypyrrole was performed on a pencil graphite electrode by cyclic voltammetry (CV) or by potential pulse sequences (PPS). The modified electrode surface was used for electrochemical determination of target‐DNA by differential pulse voltammetry. According to our best knowledge this is a first report on the application of DNA‐imprinted polymer for the determination of target‐DNA. The results showed that the molecularly imprinted polypyrrole (MIPPy) layer that formed on the carbon electrode surface was sensitive for target‐DNA, while the nonimprinted polypyrrole layer was not sensitive to the same target‐DNA. Comparison of electrodes modified using PPS and CV techniques is presented.     

Corrosion inhibition of zinc in aqueous acidic media using a novel synthesized Schiff base – an experimental and theoretical study

Abstract

The kinetics of H₂ production during Zn corrosion in 0.5?M HCl without and with various additives of N,N'-bis-(1-hydroxyphenylimine)-2,5-thiophenedicarboxaldehyde (HPTD) was studied using gasometry and electrochemical techniques. The surface of the corroded Zn samples was investigated using SEM and Optical Profilometry. The rate of H₂ production (RHP) increased with the immersion time and temperature. Presence of HPTD mitigated RHP due to an adsorption process. The electrochemical impedance spectroscopy showed that HPTD had a good inhibitive effect. Polarization data proved that HPTD acted as a surface-active mixed-type inhibitor. Some thermodynamic parameters were deduced and discussed. Theoretical calculations were also conducted to corroborate the capability of HPTD to protect Zn surface from corrosion process.     

A new reagent for the synthesis of [26]hexaphyrin: N-sulfonyl aldimine

The selective synthesis of [26]hexaphyrin(1.1.1.1.1.1) has been achieved by the reaction of meso-substituted tripyrrane and N-sulfonyl aldimine. The protocol is simple and requires only a catalytic amount of copper(II) triflate under mild reaction conditions.     

Asymmetric aldol addition of α-azido ketones to ethyl pyruvate mediated by a cinchona-based bifunctional urea catalyst

The first asymmetric synthesis of ethyl 4-aryl-3-azido-2-hydroxy-2-methyl-4-oxobutanoates via a cinchona organocatalyst induced aldol addition of α-azido ketones to ethyl pyruvate has been developed. The coupling reaction under optimized conditions was carried out to furnish tetrafunctionalized synthons with enantioselectivities of up to 91:9 and enriched diastereoselectivities of up to 95:5 (syn:anti).     

Multi-walled Carbon Nanotubes and Gold Nanorod Decorated Biosensor for Detection of microRNA-126

In this article, we introduced a novel electrochemical biosensor for the detection of microRNA-126. The biosensor utilizes a hybridization assay combined with multi-walled carbon nanotubes and gold nanorod-decorated screen-printed carbon electrodes. For electrode preparation, gold nanorods were first immobilized onto the surface of bare and multi-walled carbon nanotube-modified screen-printed carbon electrodes, and the thiol tagged-capture probe was immobilized on the electrode surface through gold and thiol group interaction. After the immobilization, thiol tagged-capture probe hybridized with the target sequence. Under optimum conditions, we determined limit of detection (LOD) and limit of quantification (LOQ) as high as 11 nM and 36 nM, respectively.     

Polyamide 6‐polycaprolactone multiblock copolymers: Synthesis,characterization, and degradation

This work describes the synthesis and characterization of polyamide 6 (PA 6)‐polycaprolactone (PCL) multiblock copolymers. Low molar mass, fully amine end‐capped PA 6 was prepared by the addition of a diamine monomer during ε‐caprolactam polymerization. A low molar mass PCL was selected to be incorporated as the biodegradable block and was fully end‐capped with toluene 2,4‐diisocyanate. End group analysis and molecular weight characterizations were performed for both end‐functionalized polymers by SEC, NMR and titration analysis. Incorporation of PCL into PA 6 was mainly achieved by solution mixing of the two end‐functional blocks and, was continued after the removal of the solvent with solid state polymerization (SSP) by gradual heating until about 40 °C below the melting temperature of the PA 6. Molecular weights started to grow immediately during solution mixing and only increased marginally during the SSP treatment. FTIR and SEC studies confirmed the reaction between the two components. DSC data, in combination with the enhanced molar mass during solution mixing pointed to a blocky microstructure, for which distinct melting and crystallization temperatures were observed for the PCL and the PA 6 blocks. Hydrolytic and enzymatic degradation studies were performed at 25 °C where the degree of degradation was followed by weight loss analysis, SEM and SEC. © 2011 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2011     

New polyphenylene‐g‐polystyrene and polyphenylene‐g‐polystyrene/poly(ε‐caprolactone) copolymers by combined controlled polymerization and cross‐coupling processes

1,4‐Dibromo‐2‐(bromomethyl)benzene and 1,3‐dibromo‐5‐(bromomethyl)benzene were used as initiators in the atom transfer radical polymerization of styrene in conjunction with CuBr/2,2′‐bipyridine as a catalyst. The resulting polystyrene (PSt)‐based macromonomers, possessing at one end a 2,5‐dibromophenylene or 3,5‐dibromophenylene moiety, were used in combination with 2,5‐dihexylbenzene‐1,4‐diboronic acid for Suzuki coupling in the presence of Pd(PPh₃)₄ as a catalyst or with the system NiCl₂/2,2′‐bipyridine/triphenylphosphine/Zn for Yamamoto polymerization. Polyphenylenes (PPs) with PSt chains as substitution groups were obtained. The same macromonomers were used in Yamamoto copolycondensation reactions, in combination with a poly(ε‐caprolactone) (PCL) macromonomer, and this resulted in PPs with PSt/PCL side chains. The obtained PPs had good solubility properties in common organic solvents at room temperature similar to those of the starting macromonomers. The new polymers were characterized with ¹H (¹³C) NMR, IR, and gel permeation chromatography. The optical properties of the polymers were monitored with UV and fluorescence spectroscopy. The thermal behaviors of the macromonomers and final PPs were investigated with differential scanning calorimetry and compared. The morphology of PPs containing PSt and PCL blocks was characterized with atomic force microscopy, and a microphase‐separated layered morphology was observed. © 2005 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 43: 879–896, 2005