Mercury arc lamp based super-resolution imaging with conventional fluorescence microscopes

We present an implementation of localization based three-dimensional super-resolution imaging on a regular microscope. We retain the original arc lamp as the photoactivation light source, and incorporate an inexpensive diode laser for imaging. As alterations to the standard microscope is minimal, this optical setup can be easily adapted in a typical research laboratory and even undergraduate teaching experiments, providing an inexpensive system for students and research scientists who require such super resolution capabilities. With this simple design, a spatial resolution of better than 40 nm at a reasonable frame rate has been achieved, adequate for most routine applications.     

DMA-80测汞仪直接测定固体样品中的汞

利用DMA-80测汞仪直接测定固体样品中的汞,采用升温加热直接进行热分解、金汞齐反应,采用长、短双检测池,可直接测定固体、液体样品,汞含量在0.n~600.0ng/g范围内的样品都能准确地测定,每个样品测定时间约为5min。测定结果证明方法具有可靠性。     

DMA-80测汞仪的应用探讨

本文的主要实验目的是利用DMA-80测汞仪直接测定固体样品中的汞并证明其方法可靠。采用升温加热直接进行热分解、金汞齐反应,采用长、短双检测池,可直接测定固体、液体样品,汞含量在0.n×10-9～600.0×10-9范围内的样品都能被很准确地测定,每个样品测定时间约为5min。 测试结果证明其方法具可靠性。     

Prussian Blue Modified Amperometric Hg2+ Ion Biosensor Based on Glucose Oxidase Inhibition

In this research a Hg²⁺ ion biosensor was developed by combining Prussian blue (PB) with glucose oxidase (GOx) – an enzyme that can be inhibited by Hg²⁺ ions. An application of PB in the design of Hg²⁺ ion biosensor enabled detecting changes in hydrogen peroxide reduction current at low operational potential of 0.2 vs Ag|AgCl,KCl_sat. The described Hg²⁺ ion biosensor exhibited wide linear range from 27 μM to 247 μM of Hg²⁺ and higher maximal detectable concentration of Hg²⁺ than other GOx inhibition-based biosensors, making it convenient for the analysis of samples with high concentration of Hg²⁺ ions.     

High Valent Mercury Fluoride Ions

Mercury fluoride ions formed during the laser ablation of HgF_{2( s )} show the formation of six different cluster ion series viz., HgF_n^±, Hg_nF_n–2^±, Hg_nF_n–1^±, (HgF)_n^±, Hg_nF_n+1^±, and Hg_nF_n+2^±. Among the different ion series, the observation of high valent HgF_n^±_(n^±_{=3,4; n}⁻_=6–8) indicates the existence of corresponding molecules which signify the remarkable participation of 5d Hg electrons in the chemical bonding with F atoms and thus make Hg a truly transition metal. Further, molecular orbital calculations show a large HOMO-LUMO energy gap (≥3 eV) and high electron affinity (≥5 eV) that indicates highly stable HgF_n=3,4,6,8 with super halogen properties.     

The simultaneous elimination of arsenic and mercury ions via hollow fiber supported liquid membrane and their reaction mechanisms: Experimental and modeling based on DFT and generating function

This study investigates the simultaneous removal of low concentrations of arsenic and mercury ions from synthetic produced water via hollow fiber supported liquid membrane (HFSLM). Results show that HFSLM can remove both arsenic and mercury ions from synthetic produced water to less than 0.02 and 0.001 mg·L^-1, respectively. These final concentrations comply with the wastewater standard of Thailand. Percentages of extraction for arsenic and mercury ions proved to be about 100 %. Those of recovery for arsenic and mercury ions reached 70 % and 75 %, respectively. A quantum model based on density functional theory (DFT) is introduced to analyze the forming and breaking of supramolecular complex species in the processes of extraction and recovery, respectively. Furthermore, the concept of Generating Function is applied to construct a mathematical model for forecasting the potential of removing metal ions via HFSLM. The mathematical model conforms to the experimental data, having an average relative deviation of 5 %. The results of this study provide a better understanding of the transport mechanisms of arsenic and mercury ions.     

催化裂解-金汞齐富集-冷原子吸收光谱仪测定土壤样品中总汞含量

为建立采用催化裂解-金汞齐富集-冷原子吸收光谱仪即直接测汞仪测定土壤样品中汞含量的方法,本研究配制汞总量为0~2 ng、0~15 ng和25~1023 ng的三种不同汞浓度系列的标准工作曲线,选取9个土壤样品,3种国家土壤有证标准物质,同一样品分别进行6组平行测定,并抽取3个土壤样品进行3种不同浓度加标回收试验,以对其方法精密度和准确度进行论证。 结果显示：仪器信号值与Hg总量之间均呈良好的线性关系。根据仪器多次测定空白数据结果,按照称样量0.1 g计算,方法最小检出量为0.09 ng/g;平行测定结果相对标准偏差均小于10%,土壤标准物质测定值与标准物质标准参考值均相符,不同浓度的加标回收率范围为78.4%～92.7%。结果表明催化裂解-金汞齐富集-冷原子吸收光谱仪,可用于批量土壤样品中汞含量的快速测定分析,方法的精密度和准确度可满足测定分析要求,且实验过程中无需前处理消煮,操作方便、快速高效。     

ICP-AES法测定烟花爆竹药物中的砷、汞、铅

为了禁止含砷、汞、铅物质应用到烟花爆竹药物中,建立ICP-AES方法,在烟花爆竹药物经乙醇、丙酮及硝酸处理后,选用As 189.042 nm、Hg 194.227 nm、Pb 220.353 nm分析线同时测定这3种元素的含量,结果:相对标准偏差为1.97%~2.71%,回收率为98.5%~101%.方法操作简单快速,检出限低、干扰小,结果准确可靠.     

一种裸眼识别Cu~(2+)和Hg~(2+)传感器的合成与性质研究

设计合成了一种香豆素修饰的罗丹明类衍生物L,用作程序型Cu2+与Hg2+检测传感器。采用核磁共振谱、高分辨质谱、红外光谱对其结构进行了表征。利用紫外光谱考察L对不同金属离子的识别作用,研究发现L可以高选择性和高灵敏度识别Cu2+。向L溶液中加入不同的金属离子时(Zn2+,Hg2+,Cu2+,Fe3+,Cd2+,Co2+,Ni 2+,Mg2+,Ca2+,Al 3+,La3+,K+,Na+,Mn2+,Pb2+和Ag+),只有Cu2+的加入会使溶液由无色变为粉色,并且在534nm出现一个新的吸收峰,说明传感器L可以裸眼识别Cu2+。通过紫外滴定得到L可以识别Cu2+的最低检测限为1.9×10-8 mol·L-1。制备了基于L的Cu2+检测试纸,该试纸可以方便快捷的检测水中的Cu2+。通过Job’s曲线和高分辨质谱确定了配合物L-Cu2+之间的结合比为1∶1。另外,若向L-Cu2+的DMSO溶液中加入1equiv.EDTA,溶液瞬间由粉色变为无色,说明EDTA可以取代L并与Cu2+结合,因此传感器L识别Cu2+是可逆的。另外,向L-Cu2+的配合物中加入Hg2+,溶液会由粉色变为无色(而加入其他阳离子没有明显变化),这可顺序实现对Hg2+的裸眼识别。通过紫外滴定计算出L-Cu2+识别Hg2+的最低检测限为2.9×10-7 mol·L-1。因此该程序型传感器L可以有序的裸眼识别Cu2+和Hg2+。     

Ultrasensitive determination of mercury by solution anode glow discharge atomic emission spectrometry coupled with hydride generation

An innovative method for the ultrasensitive detection of mercury by solution anode glow discharge atomic emission spectroscopy (SAGD-AES) coupled with hydride generation (HG) was first investigated. In this method, the mercury vapor generated by the HG was transmitted to the SAGD through the miniature hollow tungsten tube for excitation and detected by a miniaturized spectrograph. A thorough parametric evaluation of the HG and SAGD system was performed, including the type and concentration of carrier acid, He flow rate, concentrations of NaBH₄, discharge current and discharge gap. Under optimal operating conditions, the detection limit for Hg²⁺ achieved 0.03 μg/L, with a relative standard deviation of 1.1% at the Hg²⁺ concentration of 5 μg/L. Moreover, the correlation coefficient of the calibration curve was 0.9996 in the range between 0.1 and 10 μg/L. The accuracy and practicability of HG-SAGD-AES were verified by measuring GBW09101b (human hair), GBW10029 (fish), soil and rice samples. The results showed good agreement with the certified values and values from direct mercury analyzer (DMA).