Determination of five sesquiterpenoids in Xingnaojing injection by quantitative analysis of multiple components with a single marker

A quantitative analysis of multiple components with a single‐marker method was established for the simultaneous determination of five sesqutiterpenoids in Xingnaojing injection. This method was established with Xingnaojing injection determined by high‐performance liquid chromatography coupled with diode array detection. The durability and system suitability of the established method were evaluated, and the reliable relative correction factors were obtained with curdione selected as an internal reference. The contents of the five components in all Xingnaojing injections were determined by external standard method and the contents of curcumenone, curcumenol, curzerenone, and germacrone were also calculated with the obtained relative correction factors. Then, relative error was investigated to estimate the difference of the two methods. As a result, the established new method possesses good adaptability, and there is no significant difference between the two methods, except for the content of curzerenone in eight samples. To put the established method into practice, the limits of quantitation of the established method of the five components were proposed and defined. Thus, the developed methodology can also be utilized to the quality evaluation of Xingnaojing injection, in spite of the difference found in the content of curzerenone between the external standard method and the newly established method.     

Mobile relays for enhanced broadband connectivity in high speed train systems

With the introduction of wireless modems and smart phones, the passenger transport industry is witnessing a high demand to ensure not only the safety of the trains, but also to provide users with Internet access all the time inside the train. When the Mobile Terminal (MT) communicates directly with the Base Station (BS), it will experience a severe degradation in the Quality of Service due to the path loss and shadowing effects as the wireless signal is traveling through the train. In this paper, we study the performance in the case of relays placed on top of each train car. In the proposed approach, these relays communicate with the cellular BS on one hand, and with the MTs inside the train cars on the other hand, using the Long Term Evolution (LTE) cellular technology. A low complexity heuristic LTE radio resource management approach is proposed and compared to the Hungarian algorithm, both in the presence and absence of the relays. The presence of the relays is shown to lead to significant enhancements in the effective data rates of the MTs. In addition, the proposed resource management approach is shown to reach a performance close to the optimal Hungarian algorithm.     

含辅酶Q10材料的抗辐射性能及含量测定

采用高效液相色谱法测定了辅酶Q10(CoQ10)的含量,并探讨了CoQ10的防晒性能.色谱柱为Kromasil 5-C18柱(150×4.6 mm,5μm);流动相为甲醇-无水乙醇(体积比30:70),流速为1.0 mL/min;检测波长275 nm.实验考察了不同含量CoQ10在3个紫外区的防晒性能,及与维生素C或维...     

高效液相色谱法测定普卢利沙星胶囊的含量

建立高效液相色谱法测定普卢利沙星胶囊含量的方法。采用高效液相色谱法,以0.01mol.L-1盐酸溶液为溶剂,测定波长为280nm。在10—100μg.mL-1浓度范围内,主峰面积与相应的浓度呈良好的线性关系（r=0.9998）。重复测定峰面积平均值为1215.9（n=6）,相对标准偏差为0.7%。平均回收率为99.77%（n=9）,相对标准偏差为0.26%。该方法准确简便、重复性好,可作为普卢利沙星胶囊含量测定的有效方法。     

微波消解-FAAS测定皖南茶园土壤中矿质元素含量

采用微波消解-FAAS测定了皖南休宁县茶园不同深度土壤中的矿质元素Cu、Cd、Mn、Zn、Fe、Pb的含量,分析了不同深度土壤中矿质元素的分布特征,结果表明,皖南土壤中矿质元素Cu、Cd、Mn、Zn、Pb 5元素含量随土壤深度呈递减趋势,土壤表层的矿质元素(Cu、Cd、Mn、Zn、Pb)含量高于深层的,这与施肥有关,土...     

Real-time quantification of viable bacteria in liquid medium using infrared thermography

Quantifying viable bacteria in liquids is important in environmental, food processing, manufacturing, and medical applications. Since vegetative bacteria generate heat as a result of biochemical reactions associated with cellular functions, thermal sensing techniques, including infrared thermography (IRT), have been used to detect viable cells in biologic samples. We developed a novel method that extends the dynamic range and improves the sensitivity of bacterial quantification by IRT. The approach uses IRT video, thermodynamics laws, and heat transfer mechanisms to directly measure, in real-time, the amount of energy lost as heat from the surface of a liquid sample containing bacteria when the specimen cools to a lower temperature over 2 min. We show that the Energy Content (EC) of liquid media containing as few as 120 colony-forming units (CFU) of Escherichia coli per ml was significantly higher than that of sterile media (P < 0.0001), and that EC and viable counts were strongly positively correlated (r = 0.986) over a range of 120 to approximately 5 × 10⁸ CFU/ml. Our IRT approach is a unique non-contact method that provides real-time bacterial enumeration over a wide dynamic range without the need for sample concentration, modification, or destruction. The approach could be adapted to quantify other living cells in a liquid milieu and has the potential for automation and high throughput.     

新乐工业新区土壤中多种元素的测定

采用湿法消解对不同厂区的土壤进行前处理,应用电感耦合等离子体原子发射光谱法(ICP-OES)测定土壤中的金属元素含量,使用原子荧光光谱法AFS测定砷的含量,测定的相对标准偏差为0.8%～3.0%,回收率为89.0%～105.5%。实验表明,方法简单快速、准确,较高的灵敏度和较低的检出限,均能满足土壤中多元素分析的要求。     

忽地笑鳞茎中总生物碱含量的月动态变化

采用紫外分光光度法测定了不同月份忽地笑鳞茎中总生物碱含量,为确定适宜的采收时间提供实验依据。结果表明,忽地笑鳞茎中总生物碱含量随生长发育时期或月份不同存在一定差异,其中11月的最高,12月次之,1月的最低,最高值与最低值相比,约为2.6倍,差异达极显著水平(P<0.01)。精密度、稳定性实验及回收率等方法学考察表明,紫外分光光度法测定忽地笑鳞茎中总生物碱含量效果好,稳定性强,方法可靠。综合忽地笑鳞茎中总生物碱含量、中药材产量和土地利用率等因素考虑,以11月底前后采收比较适宜。     

反相高效液相色谱法测定维格列汀

建立反相高效液相色谱法测定维格列汀的含量。采用Venusil MP C18色谱柱（250mm×4.6mm,5μm）,流动相为磷酸盐缓冲溶液(称取20mmol·L^-1磷酸二氢钾,用85%浓磷酸调至pH3.0)-乙腈（88:12,V/V）,检测波长为210nm,流速为1.0mL·min^-1,柱温为25℃。维格列汀在5—500μg·mL^-1范围内与峰面积呈良好的线性关系（r=0.9999）,维格列汀的平均回收率为102.0%,RSD为2.65%。方法准确、简便,适用于维格列汀的定量分析。     

自嵌入双功能图像水印算法

利用自嵌入技术提出一种同时实现版权保护和内容认证的双功能图像水印算法.在嵌入端,首先将原始图像划分成互不重叠的子块,将各子块每个像素的最低m位置0,然后将最低m位置0后的子块进行奇异值分解,通过提取奇异值范数的最高位奇偶性产生原始鲁棒特征水印,然后再将原始鲁棒特征水印嵌入各子块每个像素的最低m位得到含水印图像.检测端从攻击的含水印图像提取鲁棒特征水印的过程与嵌入端产生原始鲁棒特征水印的过程类似,并且通过计算提取的鲁棒特征水印与原始鲁棒特征水印之间的归一化相关度进行版权鉴别,通过判断提取的鲁棒特征水印与攻击图像各子块每个像素的最低m位的一致性实现篡改检测进行内容认证.理论分析和实验结果都表明算法具有非常好的不可见性.实验结果还表明,算法不仅在抵抗添加噪音、剪切、JPEG压缩、平滑、重采样和几何攻击如随机删除行、向右偏移列、向下偏移行表现出很强的鲁棒性,而且能够精确定位出篡改位置和区分篡改类型.因此,算法具有版权保护和内容认证双重功能.