Hydrogen peroxide biosensor based on the immobilization of horseradish peroxidase on ��-Al2O3 nanoparticles/chitosan film-modified electrode

An amperometric biosensor based on horseradish peroxidase (HRP) and ??-Al₂O₃/chitosan composite film at a glassy carbon electrode has been developed. Hydrogen peroxide (H₂O₂) was detected with the aid of ferrocene monocarboxylic acid mediator to transfer electrons between the electrode and HRP. The morphology and composition of the modified electrode were characterized by scanning electron microscopy and electrochemical impedance spectroscopy. The electrochemical characteristics of the biosensor were studied by cyclic voltammetry and amperometry. The effects of HRP concentration, the applied potential, and the pH values of the buffer solution on the response of the sensor were investigated for optimum analytical performance. The proposed biosensor showed high sensitivity (0.249?A M^?1?cm^?2) and a fast response (<5?s) to H₂O₂ with the detection limit of 0.07???M. The linear response range of the enzyme electrode to H₂O₂ concentration was from 0.5 to 700???M with a correlation coefficient of 0.9998. The apparent Michaelis-Menten constant of the biosensor was calculated to be 0.818?mM, exhibiting a high enzymatic activity and affinity for H₂O₂.     

The G-Ellipticity for the Sub-Laplacian on Two Step Stratified Lie Groups

This paper reveals that the sub-Laplacian L₀ on two step stratified Lie groups has a similar behavior like elliptic operators on the Euclidean space, that is, the sub - Laplacian satisfies a group-elliptic estimate, called the G- elliptic estimate (or the L^p regularity), and the general left Invariant operator L_λ has such a behavior if and only if λ is admissible.     

积分微分扩散方程解的Blow-up性质

Cohen和C.V.Pao在[1][2]中讨论过在核反应器动力学中的方程 φ_t-▽·(D(x)▽φ)=bφ+Bφintegral from 0 to 1(φ(s,x)ds) 的解的性质,本文讨论方程##特殊公式未编改     

SYSTEM OF GENERALIZED VECTOR VARIATIONAL INEQUALITIES

1IntroductionA vector variational inequality(for short,VVI),as an important generalization of the clas-sical variational inequality,was first introduced and studied by Giannessi[6]in finite dimensionalEuclidean spaces.Later on,a VVI was studied and genera…     

带利率的更新风险模型的破产概率的上界估计

本研究了在常利率条件下普通更新风险模型的破产概率问题．采用一种递推的方法给出了这种情况下破产概率的一个上界估计．     

电子束辐照烟气脱硫脱氮反应器中的剂量分布的模拟计算

采用Monte-Carlo程序EGSnrcMP对能量为0.8MeV的电子束辐照烟气脱硫脱硝反应器中的剂量分布进行了模拟计算,将计算的结果运用MATLAB数学分析软件进行了数据处理,对能量为0.5MeV的电子束辐照烟气脱硫脱硝反应器中的剂量分布进行了模拟计算,并与文献中实验测量值进行了比较。结果表明:采用EGSnrcMP程序计算所得到的剂量与实验结果吻合。     

WAVEFUNCTIONS OF L-S COUPLING FERMION SYSTEM (Ⅱ):DOUBLE■-l

1991MRSubjectionClassification81R05,20C351IntroductionIiiapreviouspaper['].wit11avediscussedhowtoconstructthewavefllucti()llsofL-S(\011piing,single-lferlllionsystelll.Alldthecoefficientsoffi.actiollalparentage(CFP)withfixs(1seniorityharebeedcalclllatedbyarecurrentformula.Practically,withthe11alpoftileoperatorsofferrxlionpairsthegeneralwavefunctionsofmanyfermionswithsixlgle-lcouldbeobtainedfi.omthewavefullctionswithoutfermionpairs.Asaresult,therecurrentforlllulaofdeterrxliningCFPissimplif…     

胞嘧啶脱氨基化酶APOBEC3家族及其与核酸复合物结构研究进展

载脂蛋白B mRNA催化编辑蛋白APOBEC3（简称为A3）是细胞内逆转录转座子防御系统中一类家族蛋白,它通过对底物单链DNA和RNA中胞嘧啶的脱氨基化在人类的健康和疾病中发挥多种多样的作用.部分人源A3家族蛋白通过对病毒基因组中的胞嘧啶脱氨基化产生尿嘧啶,使逆转录病毒人类免疫缺陷病毒（HIV-1）基因组发生G→A碱基突变,致使HIV-1基因组不能执行正常的功能而抑制HIV-1病毒复制.作为反保护措施,HIV-1病毒利用自身的Vif蛋白（viral infectivity factor）结合人源A3蛋白,通过泛素化标记使A3蛋白降解,从而保证病毒感染.为更好理解A3蛋白催化胞嘧啶脱氨基化机制及抗病毒机制,综述了A3家族蛋白结构、它们对DNA或者RNA进行脱氨基化反应特点和它们与核酸复合物结构的研究进展,对A3家族蛋白参与脱氨基化反应的关键残基如何与核酸碱基相互作用等作了简单概括,相互作用的共同特征进行简要总结,对后期如何利用冷冻电镜技术开展全长A3蛋白相关工作做了展望.本文也部分讨论了A3蛋白如何与Vif相互作用,因此本综述对针对这些相互作用合理设计抗病毒药物有一定帮助.     

Acetate chitosan with CaCO3 doping form tough hydrogel for hemostasis and wound healing

In recent years, chitosan has been applied for wound management due to its properties of biocompatibility, biodegradability, antimicrobial activity, and low immunogenicity. But the poor water solubility in neutral pH limited its further application in clinical wound healing. To overcome this problem, acetate chitosan was developed and approved as commercial products for wound healing. However, the acidity of acetate chitosan was potentially allergenic, and the poor mechanical properties of its formed hydrogels also hindered the therapeutic efficacy in wound care. In this study, CaCO₃ was simply doped into acetate chitosan to form the wound dressing. After absorbing water, the H⁺ of acetate chitosan reacted with CaCO₃ to release Ca²⁺, resulting in acidity decreased. The production of Ca²⁺ and residue of CaCO₃ cross‐linked with chitosan to form a tough hydrogel by electrostatic interaction. The physical characteristics, swelling, mechanical testing, and blood clotting were evaluated. The results in vitro demonstrated that after doping CaCO₃ into acetate chitosan, the mechanical properties and blood clotting of the formed hydrogel were increased. Then, the evaluation of hydrogels in vivo revealed that it can also accelerate the wound healing by promoting re‐epithelization and collagen deposition. This simple way by doping CaCO₃ into acetate chitosan can increase wound healing, and it can also broad the application of acetate chitosan in clinical use.