Microchip electrophoretic separation for the fast diagnosis of Anaplasma phagocytophilum infection in cattle

We report a diagnostic method for Anaplasma phagocytophilum (A. phagocytophilum) infection in cattle using a nested PCR and microchip electrophoresis (ME). A. phagocytophilum causes human granulocytic anaplasmosis and granulocytic ehrlichiosis, which are emerging tick‐borne zoonotic diseases. Nested PCR was used to amplify genomic DNA samples extracted from cattle blood. The amplified PCR products were analyzed under a sieving gel matrix of 0.7% poly(ethyleneoxide) (M_r=8 000 000) in a conventional glass microchip. In the ME assay, A. phagocytophilum was analyzed within 35 s with a relative standard deviation of 1.30% (n=5) using a programmed field strength gradient (PFSG) as follows: 615.3 V/cm for 0–24 s, 66.7 V/cm for 24–34 s, 615.3 V/cm for 34–100 s. The ME‐PFSG assay was clinically validated by comparing the 16S rRNA gene levels obtained by this method with those measured using conventional slab gel electrophoresis performed with ten cattle blood samples suspected of A. phagocytophilum infection. In contrast to slab gel electrophoresis, the proposed ME‐PFSG methodology had increased sensitivity (200–450 pg/μL), a faster analysis time (<35 s), and required a smaller sample volume (～162 fL).     

Z箍缩等离子体X射线凸晶谱仪

 针对波长为0.3~0.5 nm的喷气箍缩等离子体X射线诊断,研制了一种适用的高空间分辨的晶体谱仪。色散元件采用云母（002）凸面晶体,布拉格角为37°,信号采用X射线胶片进行接收,有效接收面积为30 mm×80 mm。物理实验在“阳”加速器装置上进行,胶片获得了氩喷气K,L壳层光谱信号,其光谱范围较宽,为0.31~0.40 nm。经解谱发现,类氦谱线有明显的基底,用最小二乘法拟合包络曲线去噪处理后,得到类氦谱线光谱分辨力为200~300。实验结果表明,该谱仪获得的X射线测量值与理论值相符,适合喷气箍缩等离子体X射线光谱的诊断。     

辐射驱动条件下冲击波速度直接测量技术

 针对超高压下透明材料的高压离化机理,分析了透明材料中冲击波直接诊断技术的基本方法。利用Drude自由电子气模型,分析了不同冲击压力下冲击波阵面反射率的变化。设计了专门的实验,将探测器致盲区与信号区错开,获得了蓝宝石中冲击波阵面反射的信号。结果表明:冲击波速度为32 km/s时,其波阵面的反射率约为35%,致盲效应出现时间与激光脉冲峰值到达时刻相同,持续时间也与激光脉宽相同。分析了致盲应产生的原因,并提出了解决办法。给出了加蓝宝石窗口后的测速公式,经过和实验对比,确认了测速公式的正确性。     

环形电子束积分图像诊断技术

 采用图像诊断方法对高能环形电子束形状及空间尺寸进行了研究,以高能脉冲环形电子束轰击高Z靶材料产生脉冲X射线,X射线经过X射线增感屏转换为可见光,用单次图像采集系统获取可见光的积分图像。为满足诊断所需的空间分辨和系统灵敏度,通过理论计算确立了靶的材料、厚度及X射线增感屏的型号和厚度等参数。根据测试环境,设计了系统的现场安装结构,系统基本满足测试要求。分析从实验中获取的图像,可知环形电子束的内径为36.5 mm,环厚为1 mm,环形不均匀,水平方向电子束强。     

镉污染水稻高光谱诊断分析与建模

为了快速、准确地探测自然环境下水稻镉污染胁迫状况,提出了一种基于可见光-近红外光谱小波分析技术的快速识别和估算水稻镉污染的方法。根据野外实测水稻高光谱数据、水稻叶片主要生化参数及重金属含量等数据,利用Daubechies小波系的db5小波函数对350～1300nm水稻高光谱反射率进行9层分解,并提取小波能量系数进行镉含量回归建模。结果显示:第5层小波分解(d5)的奇异范围为550～810nm,奇异幅度为0.04,模极大值的中心位于700nm处,对识别水稻镉污染效果最佳;以第3层小波能量系数作为自变量的回归模型对水稻镉含量估算精度最高,其决定系数R2高达0.958,均方根误差RMSE为0.122。小波奇异性分析可以较准确的诊断水稻镉污染胁迫状况,基于小波能量系数的建模能有效估算水稻镉污染胁迫水平。     

"三明治"靶型在间接驱动冲击波实验中的应用

间接驱动方式具有提供更高冲击波压力的潜力,对冲击波物理研究来说是一种很好的驱动方式.针对间接驱动下X射线离化对透明窗口造成的致盲问题,提出了新的"三明治"靶型结构,以完成间接驱动下冲击波实验.该靶型可以用两种方法避免致盲效应对实验的干扰.一种方法是从时间上避开致盲效应,使致盲效应与冲击波测量区错开,从而获得冲击波数据.另一种方法是从强度上屏蔽X射线,将X射线阻挡在透明材料之前,使其不能产生致盲效应.对单冲击实验而言,用增加烧蚀层厚度的方法,将X射线离化效应与冲击波信号从时间上错开,获得了蓝宝石和石英晶体中冲击波实验的结果.用增加阻挡层的方法,获得了间接驱动条件下石英晶体和聚苯乙烯材料中的冲击波实验结果.提出的"三明治"靶型,为间接驱动条件下状态方程和冲击波调速技术提供了技术支持. 关键词： 冲击波 光学诊断 离化效应 干涉仪     

Universal prediction-based adaptive fault estimator applied to secure communication

In this paper, a communication scheme that could use a nonlinear dynamical system to create encrypted keys with an additional dimension is proposed, and the scheme could keep encrypted keys not to diverge. Since the divergence of encrypted keys (nonlinear signals) easily happens in non-linear systems coupled with other systems, the adaptive control approach, proposed in this paper, uses the universal state-space adaptive observer-based fault diagnosis/estimator and the high-performance tracker to eliminate the divergence of encrypted keys. At the same time, the receiver of communication retrieves informal messages by the universal state-space adaptive observer-based fault diagnosis/estimator and the high-performance tracker. Thus, this paper takes advantage of the merit of digital redesign methodology for a practical implementation of secure-communication, and the estimator solves the problem of secure communication. Thus developed a new approach could add more dimensions into nonlinear secure-communication systems without having the problem of divergence of encrypted keys.     

分层立方体网络的容错性分析

通过分层立方网络HCNn (n≥3)的容错性分析,从理论上探讨了分层立方网络基于比较模型的条件诊断度,并证明其条件诊断度为tc(HCNn)=3n?2,大约是传统诊断度(t(HCNn)=n+1)的3倍.     

广义Bayes诊断法

系统地阐述了Bayes的诊断思想,推广了多输入单输出诊断方法,对多输入多输出的诊断作出一次尝试,克服以往诊断的局限性,给出一种广泛应用的诊断方法,并通过仿真示例,表现出其可行性及有效性.     

潘宁型放电等离子体的发射光谱分析

重新设计了潘宁型等离子体源实验装置,在低气压下得到了稳定的等离子体.分析了等离子体的发射光谱,得到了等离子体光谱强度与放电气压和放电电压之间的关系,并且对氮气的发射光谱进行了分析.