改进RCF算法的电缆绝缘层边缘检测

目前电缆绝缘层厚度检测算法主要采用图像处理技术提取出绝缘层的边缘轮廓,此类算法存在绝缘层边缘过宽和边缘不连续等问题,影响了后续的检测精度.为提高绝缘层测量精度,新算法基于RCF算法进行改进,在模型的4、5阶段采用空洞卷积,增大模型的感受野;并在侧输出网络加入尺度增强模块(SEM模块)和由浅到深的级联网络,增加侧输出图像...     

基于超滤离心前处理的液相色谱-串联质谱法手性拆分人血浆中的亚叶酸和5-甲基四氢叶酸非对映异构体及其药代动力学应用

建立了液相色谱-串联质谱（HPLC-MS/MS）同时测定人血浆中的亚叶酸和5-甲基四氢叶酸两对非对映异构体的方法。血浆经蛋白质沉淀-超滤离心处理后,以甲氨蝶呤为内标,乙腈-10 mmol/L pH 8.0醋酸铵为流动相,通过手性HSA色谱柱（150 mm×4 mm,5 μm）进行梯度洗脱。亚叶酸非对映异构体在25~5000 μg/L范围内、5-甲基四氢叶酸非对映异构体在12.5~2500 μg/L范围内,线性关系均良好。本方法在灵敏度、精密度、准确度、基质效应、提取回收率、稳定性等方面均得到充分验证,并成功应用于125 mg/m²亚叶酸和62.5 mg/m²左旋亚叶酸的药代动力学研究。结果显示：在125 mg/m²亚叶酸剂量组,左亚叶酸和左旋-5-甲基四氢叶酸的血浆峰浓度（C_max）为（3137.917±408.837）和（1679.633±244.132）μg/L,从时间点0到最后可定量时间点的药物代谢动力学时间曲线下面积（AUC_0-t）为（7504.883±1185.101）和（14001.214±2868.949）μg/L;在62.5 mg/m²左亚叶酸剂量组,左亚叶酸和左旋-5-甲基四氢叶酸的C_max为（3187.917±387.298）和（1739.204±224.755）μg/L,AUC_0-t为（7426.664±854.825）和（14884.331±1843.353）μg/L。两剂量组主要药物代谢动力学参数均无显著差异,特征一致,吸收的速度和程度一致,能够为后期进行左亚叶酸钠生物等效性研究提供技术支持。     

ICF装置靶场结构总体稳定性设计

 通过分析光学元件的动力学响应,计算靶场每条光路的结构漂移误差,进行了靶场结构总体稳定性评估。神光主机装置单光路靶场结构漂移误差计算结果表明：其靶场结构设计满足稳定性要求,该方法可以应用在ICF装置靶场结构总体稳定性设计中,但所得的漂移误差的裕度应大于一个给定的合理值。     

一种高智能化的通用自动胶片判读仪

自动通用胶片判读仪是一种高智能化的精密光学测试设备,采用了计算机控制飞点扫描技术、精密光学测量技术、图象跟踪测量与信息处理技术。飞点管分辨率达4096×4096象元,通过光学系统胶片上获得6.55μm高分辨率,飞点扫描方式灵活多样且可随意控制,通用于目前我国靶场所有的电影经纬仪和高速摄影机35mm胶片的数据判读。具有自动判读和半自动判读两种工作模式,自动判读的速度为5帧/秒,自动判读的精度为σ=±0.011mm,半自动判读的精度为σ=±0.009mm,测量数据可以记录、打印和显示。     

Biosynthesis of Hybrid Neutral Lipids with Archaeal and Eukaryotic Characteristics in Engineered Saccharomyces cerevisiae

Discovery of the Asgard superphylum of archaea provides new evidence supporting the two-domain model of life: eukaryotes originated from an Asgard-related archaeon that engulfed a bacterial endosymbiont. However, how eukaryotes acquired bacterial-like membrane lipids with a sn-glycerol-3-phosphate (G3P) backbone instead of the archaeal-like sn-glycerol-1-phosphate (G1P) backbone remains unknown. In this study, we reconstituted archaeal lipid production in Saccharomyces cerevisiae by expressing unsaturated archaeol-synthesizing enzymes. Using Golden Gate cloning for pathway assembly, modular gene replacement was performed, revealing the potential biosynthesis of both G1P- and G3P-based unsaturated archaeol by uncultured Asgard archaea. Unexpectedly, hybrid neutral lipids containing both archaeal isoprenoids and eukaryotic fatty acids were observed in recombinant S. cerevisiae. The ability of yeast and archaeal diacylglycerol acyltransferases to synthesize such hybrid lipids was demonstrated.     

Synthesis of medium‐bandgap π‐Conjugated polymers based on isomers of 5‐Alkylphenanthridin‐6(5H)‐one and 6‐Alkoxylphenanthridine

Two novel dibromo monomers consisting of the isomers of 5‐alkylphenanthridin‐6(5H)‐one (PN) and 6‐alkoxylphenanthridine (PO) were synthesized through alkylation of the precursor 3,8‐dibromophenanthrindi‐6(5H)‐one, where the molecular structures were confirmed by NMR spectroscopy. The medium bandgap conjugated polymers PDBTPN and PDBTPO were constructed by utilizing such two isomers PN and PO as the electron‐donating units and dithiophenebenzo[2,1,3]diathiazole as the electron‐accepting unit. The resulting polymers exhibited analogous absorption profiles with optical bandgap of 1.90 eV, while PDBTPO showed slightly higher absorption coefficiency. Cyclic voltammetry measurements revealed that these polymers had relatively deep highest occupied molecular orbital levels of about ?5.70 eV. Polymer solar cells based on such two polymers showed relatively high open‐circuit voltage of about 0.90 V. All devices exhibited moderate performances with the best power conversion efficiency of 3.77% achieved based on PDBTPO. Devices based on PDBTPO showed slightly higher power conversion efficiency than those based on PDBTPN, which can be ascribed to higher hole mobility and more favorable film morphology of the former. © 2016 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2016 , 54, 2119–2127     

基于有机硅组分交联稳定纳米胶束的研究进展

综述了基于有机硅组分交联稳定纳米胶束的研究进展,对制备方法和交联区域的选择以及应用领域的研究进行了较为详细的阐述.指出将有机硅引入双亲性高分子链中,利用有机硅部分的溶胶-凝胶转变对双亲高分子胶束的具体区域进行交联,能够使其胶束结构固定下来并形成杂化材料,在提高高分子胶束稳定性的同时又赋予其无机材料的刚性、低生理毒性、便于纯化等优点,为双亲性高分子的制备以及药物的运载、控制释放等带来极大的帮助.