Determination of DNA using sodium 9,10-anthraquinone-2-sulfonate as an in situ photochemical fluorescence probe

An in situ photochemical fluorescence probe method for the determination of DNA with sodium 9,10-anthraquinone-2-sulfonate (AQS) as a photochemical fluorescence probe was developed. It was based on the conversion of AQS into an intensively fluorescent product by irradiating with UV radiation. The photochemical reaction is retarded by DNA. The determination can be carried out by measuring the fluorescence intensity at a fixed time. The calibration graph was linear in the range 0–80 ng ml⁻¹ calf thymus (CT) DNA (r = 0.9991), the limit of detection was 3.2 ng ml⁻¹ CT DNA (n = 9). The kinetic behaviour of the photochemical reaction and the effects of experimental conditions were investigated and discussed in detail. The results of absorption spectra and competitive binding experiments suggested the interaction between AQS and DNA to be intercalative.     

Photochemical synthesis of a palladium dichloromethyl complex, {(hexyl)HC(N-methyl-imidazol-2-yl)2}Pd(CHCl2)Cl.: X-ray molecular structures of {(hexyl)HC(N-methylimidazolyl-2-yl)2}Pd(X)Cl, X=Cl, and CHCl2

The reaction of (hexyl)HC(mim)₂ (1, mim=N-methyl-imidazol-2-yl) with (cod)PdMeCl in C₆H₆ yields {(hexyl)HC(mim)₂}Pd(Me)Cl (3). The photochemical reaction of 3 with CH₂Cl₂ at 23 °C in ambient room light yields {(hexyl)HC(mim)₂}Pd(CHCl₂)Cl (4). It is proposed that this reaction proceeds by homolytic scission of the PdMe bond of 3.     

纳米银胶的光化学制备及其共振散射光谱研究

采用紫外光化学法制备了银胶 ,它的最强共振散射峰在 470nm处 ,最大吸收峰为 412 2nm。在共振散射波长 470nm处 ,银浓度在 0 45 6～ 9 12 μg·mL-1范围内与所得银胶的共振散射光强度呈良好线性关系。首次采用光化学法合成了稳定的蓝色银胶 ,其最强共振散射峰位于 470nm ,在 397 4和 6 2 3 4nm处有两个较强的吸收峰。     

Photochemical behaviour of polyacryloylacetone;and polyethylacrylacetate monolayers at the air–water interface

The surface organization of enol units of polyacryloylacetone (PAA) and polyethylacrylacetate (PEAA) monolayers at the air–water interface is examined using surface pressure, surface potential and rheological measurements and theoretical calculations based on molecular models. The mechanism and kinetics of the photochemical enol–keto tautomerization of PAA and PEAA polymers organized in a monolayer of closely packed monomer units are studied by measuring the surface area increase at constant surface pressure. The results indicate an increase in the area per unit during the consecutive enol-to-keto photoconversion and the slow interfacial reorganization of these ¶forms to a more favourable state.     

富勒烯[60]的光化学反应研究

从光物理出发,综述了近几年富勒烯「６０」的光化学反应研究进展。Ｃ６０能发生诸多的光化学反应：（１）光氧化;（２）光氢化还原;（３）「２＋２」光环化加成;（４）与叔胺的光加成;（５）与氨基酸的光加成;（６）与金属有机化合物的光加成。     

含螯合型双膦配体羰基Fe（Ⅱ）化合物紫外—可见光谱性能的研究

地双膦配体羰基铁化合物的紫外－可有收峰作发属,讨论了确定金属有机化合物ＵＶ－ＶＩＳ、Ｍ－ＬＣＴ带的方法,研究了卤素对Ｍ－ＬＣＴ带,ｄ－ｄ跃迁吸收的影响以及ＣＴ带的浓度效应。     

光化学荧光分析法研究(Ⅵ)——混合色素的光化学速差动力学荧光分析法测定

以光化学荧光衍生化法测定苋菜红(AM)和直接耐晒嫩黄5GL(DY)2种偶氮色素,研究了同时测定这2种色素混合物的光化学速差动力学荧光分析的原理和方法。AM和DY的浓度分别在0～220ng/mL和0～120ng/mL范围内呈良好的线性关系,相关系数皆为0.999,检出限为0.64ng/mLAM和0.045ng/mLDY。相对标准偏差分别为1.6%(n=8)和1.8%(n=7)。该法用于人工合成混合样品的测定效果良好。     

偶氮苯的光化学顺反异构化反应及其在光强测定中的应用

本文叙述了用偶氮苯露光计测定光强的原理。本方法也同样可用于测定A<﹦>B型光化学异构化反应的量子产率,设计了专用的计算机程序来处理在光强和量子产率的测定中的各种有关数据。     

Studies on the Determination of the Micro Components by Photochemical Kinetics

The iodides can be oxidated to iodine by O_2 in air because of their lower redox potential. In an acidic solution, this reaction can be accelerated by ultraviolet light. Because the iodine yielded from potassium iodide by light irradiating solution can react with other oxidizer components, the iodine is constantly consumed. When it arrives the terminal points of the reaction, the iodine appears. According to the reaction time of the iodine appearing the quantities of the components are determined. And the terminal points can be pointed out by the blue of the iodine-starch or potentiometric     

Clay minerals as photochemical reaction fields

This article surveys the intercalation characteristics of a number of clay minerals which can be utilized as host materials for photofunctional organic–inorganic hybrid systems as well as photochemical anisotropic reaction fields. The introduction of this review describes the chemical compositions and the structural features of various kinds of artificial as well as naturally produced clays. The chemical structures of these clays are often considered to be complicated due to their unfamiliar names which are sometimes taken from the areas where they originate although, structurally, only slight differences actually exist. The second chapter summarizes the adsorption and aggregation properties of typical organic dyes in clays. The aggregation behavior is sensitive to the kind and amount of metallic ions present in the lattice of the clays, the amount of adsorbed water as well as the structure of the organic guests to be intercalated. All of these factors are discussed in relation to the adsorption and aggregation behavior. In the third chapter, the photochemical reactions of excited metal complexes adsorbed in clays are discussed. Electron transfers for [Ru(bpy)₃]²⁺ in clays are described, showing that the clay layers provide reaction fields which stabilize the resulting charge separated species. This article aims to provide important insights into the fundamental mechanisms and properties of anisotropic reaction fields for the applications in design of promising, new photochemical and photobiological systems and processes.