Electrochemical Analysis of Glycoprotein Samples Prepared on a Pneumatically‐controlled Microfluidic Device

In this work, we designed and manufactured a microfluidic device for isolation and purification of glycoprotein samples. The conceived sample preparation device was fabricated in polycarbonate by micro‐milling. The flow control and the fluid dosage into the micro‐channels was solved by equipping the device with integrated pneumatic valves. The biochemical functionality was provided by beaded support modified by molecules with affinity to glycoproteins which was stacked inside the micro‐channel reminiscent of packed affinity columns used in glycoprotein lectin assays. Unlabeled glycoproteins, namely fetuin, asialofetuin, and prostate‐specific antigen, were voltammetrically analyzed using catalytic peak H at silver amalgam electrode.     

基于规则学习的传统纹样统一生成模式研究

传统纹样的生成是图案生成领域最具代表性的研究方向之一，从传统纹样的构图规则出发生成纹样是最常用的方法之一。以传统纹样为研究对象，针对现有传统纹样生成方法泛化能力差的问题，通过对不同传统纹样进行特征分析，找出其生成规则的异同，提出了一种传统纹样统一生成模式：寓意嵌入的层次迭代生成模式。选取几何纹、动物纹、植物纹中的典型纹样，利用统一生成模式进行重构，验证了模式的有效性。实验结果表明，统一生成模式适合多种传统纹样的重构，一定程度上是对传统纹样重构的创新应用。     

A database for inventory of proteoform profiles: “2DE-pattern”

The human proteome is composed of a diverse and heterogeneous range of gene products/proteoforms/protein species. Because of the growing amount of information about proteoforms generated by different methods, we need a convenient approach to make an inventory of the data. Here, we present a database of proteoforms that is based on information obtained by separation of proteoforms using 2DE followed by shotgun ESI–LC–MS/MS. The database's principles and structure are described. The database is called “2DE-pattern” as it contains multiple isoform-centric patterns of proteoforms separated according to 2DE principles. The database can be freely used at http://2de-pattern.pnpi.nrcki.ru .     

Transport properties and typical electron orbits in 2DEG under a local gradient magnetic field

Local current information in nanodevices is very helpful for good understanding the fundamental physical phenomena. In this work, the electron motion in 2DEG under a local gradient magnetic field is studied by the recursive non-equilibrium green's function method. The considered structure is a sandwiched configuration, lead-device-lead. For clearly observing the electron trajectories, we use the wide and narrow leads to contact the device respectively. The results show that in wide lead case, the quantized conductance plateaus with oscillations are found. These oscillations might be related to the resonant transmission in longitudinal electronic states. We also observe several typical snake electron orbits when the corresponding incident energy locates at the odd conductance plateaus, because there is exactly one opened transport channel around the horizontal middle line of device. When we shrink the width of leads and let electrons inject only from the lower half of device, the conductance suppression is very significant due to the energy level broadening in center device. In this case, we can find clear S-shape and 8-shape electron orbits at a very wide range of energy. These results can help us to understand the basic physics in 2D electron systems and may guide the design of future photoelectric device.     

基于裂纹附加模态Timoshenko梁的裂纹损伤识别

将梁中横向裂纹等效为无质量扭转弹簧，并忽略其对梁剪切变形的影响，得到的具有任意裂纹数目Timoshenko 梁自振模态的统一显示解析表达式．将裂纹梁的自振模态分为基本模态和裂纹附加模态，利用最小二乘拟合，建立了利用裂纹附加模态函数的梁裂纹损伤识别方法．通过数值模拟开展了简支单裂纹梁以及悬臂和固支双裂纹梁等的裂纹损伤识别，考察了测量误差对损伤识别的影响，数值结果表明本文所提出的裂纹损伤识别方法对裂纹位置的识别精度高于对裂纹损伤程度的识别精度；随着测量误差的增加，裂纹位置及裂纹损伤程度的识别误差增加，但仍在可接受的范围内，故该裂纹损伤识别方法在实际工程中具有一定的应用价值．     

基于图像处理的零件二维尺寸检测方法

为了提高零件二维尺寸测量精度和效率,提出一种基于图像处理的非接触式检测方法。对零件图像进行灰度化、采用自适应中值滤波算法来减小图像数据量以及除去噪声;通过最大类间方差法自动确定阈值获得二值图像;采用形态学梯度算子提取图像边缘,并用最小二乘法拟合像素尺寸;通过标准件法对系统进行标定获得零件尺寸。以6204-Z型深沟球轴承作为实验对象,获得轴承内、外径的尺寸。与游标卡尺测量结果比较,所提出的图像处理方法获得的内、外径平均误差达到9.6μm和0.7μm。通过实验对比与分析,方法快速有效,具有较高的准确性和良好的鲁棒性。     

The approachability ideal without a maximal set

We develop a forcing poset with finite conditions which adds a partial square sequence on a given stationary set, with adequate sets of models as side conditions. We then develop a kind of side condition product forcing for simultaneously adding partial square sequences on multiple stationary sets. We show that certain quotients of such forcings have the ${\omega }_1$-approximation property. We apply these ideas to prove, assuming the consistency of a greatly Mahlo cardinal, that it is consistent that the approachability ideal $I[{\omega }_2]$ does not have a maximal set modulo clubs.     

AUTOMATIC IDENTIFICATION OF MESOSCOPIC PARAMETERS OF DISCRETE ELEMENTS OF SAND MATERIAL 1)

本文依照四川大学某理论力学教学班实际教学改革的经验,通过具体举例说明计算机技术在理论力学教学中尚未开发的巨大潜力,减小数学运算使学生重点关注理论力学基本原理,批量求解使学生提前接触实际应用案例,普适性求解使学生深化对处理多变量问题的认识。同时本文还探讨了理论力学教学改革的方向和目的,即把教学重点放在普适的教学方法上,借助计算机求解多刚体、多变量问题,以期有助于理论力学教材更好地适应时代发展。     

方波信号的傅里叶分解实验

选择通用设备，建立了一个简易的周期信号傅里叶分析系统，通过其测试了方波信号各谐波成份的频率和幅度值，验证了方波信号的傅氏展开关系．