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排序方式: 共有122条查询结果,搜索用时 31 毫秒
31.
Endo M Wang H Fujitsuka M Majima T 《Chemistry (Weinheim an der Bergstrasse, Germany)》2006,12(14):3735-3740
The effect of pyrenes introduced into a tobacco mosaic virus (TMV) coat protein monomer on the formation and stability of the TMV assembly was investigated. The possible arrangement of the pyrenes in the inner cavity of the TMV rod was also estimated. The pyrene derivative was introduced to four specific amino acids in the cavity of the TMV rod structure. Rod-structure formation was examined by atomic force microscopy (AFM). Two pyrene-attached mutants (positions 99 and 100) assembled to increase the length of the rod structures by 2.5 microm at pH 5.5. The interaction of the pyrene moieties in the TMV cavity was investigated by steady-state and time-resolved spectroscopic analysis. Strong excimer emission with significantly short wavelength (465 nm) was observed from the two mutants mentioned above. Excitation and UV-visible spectra indicate that the pyrene moieties form pi-stacked structures in the TMV cavity. Details of the pyrene interaction were investigated by analyzing the fluorescence lifetime of the excimer. Results suggest that the pyrenes formed preassociated rigid structures with partially overlapped geometry in the restricted space of the TMV cavity. The pyrenes effectively stabilize the TMV rod through a pi-stacking interaction in a well-ordered way, and the single pyrene moiety introduced into the monomer affects the overall formation of the TMV rod structure. 相似文献
32.
《Arabian Journal of Chemistry》2022,15(9):104110
In this study, a series of trifluoromethyl pyrimidine derivatives 5a-5v were designed and synthesized. All synthetic compounds were original. Bioassay results showed that some of the target compounds were proved to have higher antiviral and antifungal activities than those of commercial agents. Especially, EC50 values of the curative activity of compound 5j and the protection activity of compound 5m were 126.4 and 103.4 µg/mL, respectively, which were lower than that of ningnanmycin. Microscale thermophoresis experiment proved that there was a good interaction between compound 5m and TMV-CP. Meanwhile, the antifungal activity results showed that compound 5u had a significant on in vitro against Rhizoctonia solani (RS) activity, with the EC50 value of 26.0 µg/mL, which was equal to that of azoxystrobin. As well, in vivo experiments on rice leaves showed that compound 5u could effectively control RS, and the effect of 5u on the cell morphology of RS was observed by scanning electron microscopy. 相似文献
33.
Colloidal gold has been coupled to a mutant cowpea mosaic virus (CPMV), which contains 60 cysteine residues on the surface. A purification process was developed to separate the gold-containing viral nanoblocks (VNBs) from the free gold. Agarose electrophoresis was utilized to separate the mixture followed by electroelution of the desired sample to recover the intact virus. Mobility of Au-VNB and free colloidal gold was facilitated by the addition of thioctic acid (TA). 30% of the gold-containing virus was recovered after electroelution as determined by absorbance measurements. Histogram analysis of transmission electron microscopy (TEM) images demonstrated the efficient separation of gold-containing virus from free gold. TEM and scanning electron microscopy (SEM) images indicated that the virus was recovered intact. Monodisperse spherical particles of nominal size of 45 nm were observed under SEM. 相似文献
34.
Electrochemical studies of 1,5-dihydroxynaphthalene as substrate for voltammetric enzyme immunoassay
JIAO Kui YAO Hong XU Jin & ZHANG Shusheng College of Chemistry Molecular Engineering Qingdao University of Science Technology Qingdao China 《中国科学B辑(英文版)》2004,47(3):184-191
Immunoassay is one of the biochemical analytical techniques using the specific antigen antibody com-plexation for analytical purposes. It has extensive ap-plication in clinical diagnostics, prevention and cure of diseases, and virus diagnostics. The presentation and progress of immunoassay methodology are one of the greatest achievements of bioanalytical chemistry. It is estimated that several-hundred millions of immuno-analytical determinations are carried out every year all over the world. E… 相似文献
35.
N. é. Mikhailovskii 《Mathematical Notes》1998,63(1):72-83
Asymptotic estimates of the form mrA = O(InN · ln
d
ɛ
−1), whered is the dimension of the initial space, for mosaic ranks of discrete analog of Calderón-Zygmund operators are obtained for
various mosaic covers.
Translated fromMatematicheskie Zametki, Vol. 63, No. 1, pp. 81–94, January, 1998. 相似文献
36.
Dickert FL Hayden O Bindeus R Mann KJ Blaas D Waigmann E 《Analytical and bioanalytical chemistry》2004,378(8):1929-1934
Surface imprinting techniques on polymer-coated quartz-crystal microbalances (QCM) have been used to detect tobacco mosaic viruses (TMV) in aqueous media. Molecularly imprinted polymers (MIP), tailor-made by self organisation of monomers around a template (TMV), were generated directly on the gold electrodes. Imprinted trenches on the polymer surface mimicking the shape and surface functionality of the virus serve as recognition sites for re-adsorption after washing out of the template. The sensors are applicable to TMV detection ranging from 100 ng mL–1 to 1 mg mL–1 within minutes. Furthermore, direct measurements without time-consuming sample preparation are possible in complex matrices such as tobacco plant sap.Dedicated to Professor Dr. W. Fresenius on the occasion of his 90th birthday 相似文献
37.
The mosaic structure in a Ni-based single-crystal superalloy is simulated by
molecular dynamics using a potential employed in a modified analytic embedded atom
method. From the calculated results we find that a closed three-dimensional misfit
dislocation network, with index of $\langle 011\rangle${\{}100{\}} and the side
length of the mesh 89.6\,{\AA}, is formed around a cuboidal $\gamma '$ precipitate.
Comparing the simulation results of the different mosaic models, we find that the
side length of the mesh only depends on the lattice parameters of the $\gamma $ and
$\gamma '$ phases as well as the $\gamma $/$\gamma '$ interface direction, but is
independent of the size and number of the cuboidal $\gamma '$ precipitate. The
density of dislocations is inversely proportional to the size of the cuboidal
$\gamma '$ precipitate, i.e.~the amount of the dislocation is proportional to the
total area of the $\gamma $/$\gamma '$ interface, which may be used to explain the
relation between the amount of the fine $\gamma '$ particles and the creep rupture
life of the superalloy. In addition, the closed three-dimensional networks assembled
with the misfit dislocations can play a significant role in improving the mechanical
properties of superalloys. 相似文献
38.
Abstract The change in microstrains ε, block sizes L and in the temperature dependences of conductivity of polysilicon with the grain size 30-40nm at N+, Ne+, P+ ion irradiation has been studied. It is shown that ε increases while L practically is not changing up to amorphization. The change in conductivity is governed by an increase in the density of states near the Fermi level and depends both on the damage rate for the given ions and their chemical activity. 相似文献
39.
40.
《Mendeleev Communications》2022,32(6):831-833
The interaction of the tobacco mosaic virus with the active surface of an iron-containing adsorbent obtained from lignin by the plasma-catalytic method was studied by IR spectroscopy. The results revealed decomposition of the virus into protein molecules and RNA reacting with the lignin surface via oxygen atoms during the binding of the virus to the sorbent surface. It was assumed that oxygen carboxylate groups interrelate with nanosized iron clusters incorporated into the adsorbent surface structure. 相似文献