An immunoproteomic approach for characterization of the outer membrane proteins of Salmonella Gallinarum

Salmonella enterica serovar Gallinarum (SG) is an important pathogen that causes fowl typhoid in chickens. In order to investigate SG outer membrane proteins (OMPs) as potential vaccine candidate proteins, we established a proteomic map and database of antigenic SG‐OMPs. A total of 174 spots were detected by 2DE. Twenty‐two antigen‐reactive spots were identified as nine specific proteins using PMF. OmpA was the most abundant protein among all of the identified OMPs, and it exhibited seven protein species. We conducted Western blot analysis for the SG‐OMPs in order to determine which proteins were cross‐reactive to the serovars Salmonella Enteritidis, Salmonella Typhimurium, and SG. Our results indicated that OmpA was considered to be an antigenic cross‐reactive protein among the three serovars. This study sheds new light on our understanding of cross‐protection among Salmonella serovars.     

革兰氏阴性菌WaaP蛋白的表达与纯化对比研究

采用基因重组方法构建来源于大肠杆菌和铜绿假单胞菌的waa P基因的克隆,利用多种感受态细胞表达出带有不同纯化标签的可溶性Waa P蛋白,并利用亲合层析和凝胶过滤层析对可溶性Waa P蛋白进行纯化,用SDS-PAGE进行检测。对比大肠杆菌和铜绿假单胞菌中Waa P的表达和纯化结果,为蛋白结晶选取能够得到大量稳定和高纯度Waa P蛋白的表达纯化方法,并用该方法,使用硒代甲硫氨酸培养基表达出硒代甲硫氨酸标记的Waa P,为蛋白结构解析时相位的确定提供依据。     

Application of residue distribution along the sequence for discriminating outer membrane proteins

Discriminating outer membrane proteins from other folding types of globular and membrane proteins is an important problem both for detecting outer membrane proteins from genomic sequences and for the successful prediction of their secondary and tertiary structures. In this work, we have systematically analyzed the distribution of amino acid residues in the sequences of globular and outer membrane proteins. We observed that the occurrence of two neighboring aliphatic and polar residues is significantly higher in outer membrane proteins than in globular proteins. From the information about the dipeptide composition we have devised a statistical method for discriminating outer membrane proteins from other globular and membrane proteins. Our approach correctly picked up the outer membrane proteins with an accuracy of 95% for the training set of 337 proteins. On the other hand, our method has correctly excluded the globular proteins at an accuracy of 79% in a non-redundant dataset of 674 proteins. Furthermore, the present method is able to correctly exclude alpha-helical membrane proteins up to an accuracy of 87%. These accuracy levels are comparable to other methods in the literature. The influence of protein size and structural class for discrimination is discussed.     

外周天线LHCⅡ的荧光光谱特性

外周天线LHC在光合作用过程中,担负着吸收和传递光能的作用.我们采用扫描成象荧光光谱技术对菠菜中外周天线LHC的荧光光谱特性进行了研究,在514.5nm的激光激励下获取了积分荧光谱,认为从类胡萝卜素分子到叶绿素分子间存在有能量传递.采用高斯组分光谱解析的方法,解析出LHC的荧光发射有七个谱带:656.7、664.6、671.5、677.2、683.5、689.6、695.3nm,各自所占的比例分别为3.0%、13.1%、13.3%、21.1%、13.2%、33.3%、3.0%,其中658.7nm的发射谱是由叶绿素b分子所发射的,其余的发射谱分别是由吸收峰为662、670/671、676、680nm以及吸收大于690nm的叶绿素a分子所发射.3.0%的叶绿素b分子的荧光发射说明在能量平衡过程中绝大部分能量被叶绿素a分子所禁锢,689.6nm处的荧光所占的比例最大,可能与LHC的一种自保护机制有关     

关于s－维Hausdorff测度的注记

本完善了s-维Hausdorff测度当s＝0时的一种定义形式，证明了修改后的定义与另外几种定义方式的等价性，从而得出结论：不同献中对应于不同定义方式的结论是一致的。     

QUASI-LOCAL CONJUGACY THEOREMS IN BANACH SPACES

Let f : U(x0) (?) E→F be a. C1 map and f'(X0) be the Prechet derivative of /fat X0. In local analysis of nonlinear functional analysis, implicit function theorem, inverse function theorem, local surjectivity theorem, local injectivity theorem, and the local conjugacy theorem are well known. Those theorems are established by using the properties: f'(x0) is double splitting and R(f'(x))∩N(T0 ) = {0} near X0. However, in infinite dimensional Banach spaces, f'(x0) is not always double splitting (i.e., the generalized inverse of f'(xo) does not always exist), but its bounded outer inverse of f'(x0) always exists. Only using the C1 map f and the outer inverse T0# of f'(x0), the authors obtain two quasi-local conjugacy theorems, which imply the local conjugacy theorem if X0 is a locally fine point of f. Hence the quasi-local conjugacy theorems generalize the local conjugacy theorem in Banach spaces.     

Mapping properties that preserve convergence in measure on finite measure spaces

Given a finite measure space (X,M,μ) and given metric spaces Y and Z, we prove that if is a sequence of arbitrary mappings that converges in outer measure to an M-measurable mapping and if is a mapping that is continuous at each point of the image of f, then the sequence g○fn converges in outer measure to g○f. We must use convergence in outer measure, as opposed to (pure) convergence in measure, because of certain set-theoretic difficulties that arise when one deals with nonseparably valued measurable mappings. We review the nature of these difficulties in order to give appropriate motivation for the stated result.     

TMBETADISC-RBF: Discrimination of beta-barrel membrane proteins using RBF networks and PSSM profiles

Discriminating outer membrane proteins (OMPs) from other folding types of globular and membrane proteins is an important task both for identifying OMPs from genomic sequences and for the successful prediction of their secondary and tertiary structures. We have developed a method based on radial basis function networks and position specific scoring matrix (PSSM) profiles generated by PSI-BLAST and non-redundant protein database. Our approach with PSSM profiles has correctly predicted the OMPs with a cross-validated accuracy of 96.4% in a set of 1251 proteins, which contain 206 OMPs, 667 globular proteins and 378 alpha-helical inner membrane proteins. Furthermore, we applied our method on a dataset containing 114 OMPs, 187 TMH proteins and 195 globular proteins obtained with less than 20% sequence identity and obtained the cross-validated accuracy of 95%. This accuracy of discriminating OMPs is higher than other methods in the literature and our method could be used as an effective tool for dissecting OMPs from genomic sequences. We have developed a prediction server, TMBETADISC-RBF, which is available at http://rbf.bioinfo.tw/~sachen/OMP.html.     

Kazhdan groups with infinite outer automorphism group

For each countable group we produce a short exact sequence where has a graphical presentation and is f.g. and satisfies property .

As a consequence we produce a group with property  such that is infinite.

Using the tools developed we are also able to produce examples of non-Hopfian and non-coHopfian groups with property .

One of our main tools is the use of random groups to achieve certain properties.

     

On the convexity of a class of quadratic mappings and its application to the problem of finding the smallest ball enclosing a given intersection of balls

We consider the outer approximation problem of finding a minimum radius ball enclosing a given intersection of at most n − 1 balls in . We show that if the aforementioned intersection has a nonempty interior, then the problem reduces to minimizing a convex quadratic function over the unit simplex. This result is established by using convexity and representation theorems for a class of quadratic mappings. As a byproduct of our analysis, we show that a class of nonconvex quadratic problems admits a tight semidefinite relaxation.