An efficient method for recovery of target ssDNA based on amino-modified silica-coated magnetic nanoparticles

In this paper, an improved recovery method for target ssDNA using amino-modified silica-coated magnetic nanoparticles (ASMNPs) is reported. This method takes advantages of the amino-modified silica-coated magnetic nanoparticles prepared using water-in-oil microemulsion technique, which employs amino-modified silica as the shell and iron oxide as the core of the magnetic nanoparticles. The nanoparticles have a silica surface with amino groups and can be conjugated with any desired bio-molecules through many existing amino group chemistry. In this research, a linear DNA probe was immobilized onto nanoparticles through streptavidin conjugation using covalent bonds. A target ssDNA(I) (5′-TMR-CGCATAGGGCCTCGTGATAC-3′) has been successfully recovered from a crude sample under a magnet field through their special recognition and hybridization. A designed ssDNA fragment of severe acute respiratory syndrome (SARS) virus at a much lower concentration than the target ssDNA(I) was also recovered with high efficiency and good selectivity.     

土壤源热泵系统冷凝热回收的优化研究

文中提出了一种土壤源热泵冷凝热回收系统,能够减少冬夏两季土壤源热泵系统从土壤冷热源排放吸收热量不均对土壤温度场所造成的影响,并结合火用平衡方程建立了土壤源热泵系统冷凝热回收的优化模型。基于此模型,探讨了系统冷凝器和地下埋管的优化配置,通过模拟计算对该系统在制冷工况下改进前后的运行情况进行了分析对比,结果显示土壤源冷凝热回收系统应用于实际工程能够提高整个系统的COP以及火用效率。     

The flipped longitudinal polarization sequence

By flipping the longitudinal magnetization with a chain of 180° pulses it is possible to effectively restore the effects of relaxation so that the same longitudinal magnetization is periodically recovered. The pulse sequence for achieving this, called Flipped LOngitudinal Polarization (FLOP), can be incorporated into any pulse sequence whenever it is desired to stop the attenuation in longitudinal magnetization caused by relaxation. We illustrate its use for fast, single-shot measurements of the longitudinal relaxation time and for three-dimensional T₁ mapping.     

Bio-energetic impairment in human calf muscle in thyroid disorders: a P MRS study

Mitochondrial metabolism particularly oxidative phosphorylation is greatly influenced by thyroid hormones. Earlier studies have described neuromuscular symptoms as well as impaired muscle metabolism in hypothyroid and hyperthyroid patients. In this study, we intend to look in to the muscle bioenergetics including phosphocreatine recovery kinetics based oxidative metabolism in thyroid dysfunction using in vivo ³¹P nuclear magnetic resonance spectroscopy (MRS). ³¹P MRS was carried out at resting state on 32 hypothyroid, 10 hyperthyroid patients and 25 control subjects. Nine out of 32 hypothyroid patients and 17 out of 25 control subjects under went exercise protocol for oxidative metabolism study and performed plantar flexion exercise while lying supine in 1.5 T magnetic resonance scanner using custom built exercise device. MRS measurements of inorganic phosphate (Pi), phosphocreatine (PCr), phosphodiesters (PDE) and adenosine triphosphate (ATP) of the calf muscle were acquired during rest, exercise and recovery phase. PCr recovery rate constant (k_PCr) and oxidative capacity were calculated by monoexponential fit of PCr versus time (t) at the beginning of recovery. During resting condition in hypothyroid patients, PCr/Pi ratio was reduced whereas PDE/ATP and Pi/ATP were increased. However, in case of hyperthyroidism, an increased PCr/Pi ratio and reduced PDE/ATP and Pi/ATP were observed. The results confirmed differential energy status of the muscle due to increased or decreased levels of thyroid hormone. Our results also demonstrate reduced oxidative metabolism in hypothyroid patients based on PCr recovery kinetics. PCr recovery kinetics study after exercise revealed decreased PCr recovery rate constant (k_PCr) in hypothyroid patients compared to controls that resulted in decrease in oxidative capacity of muscle by 50% in hypothyroids. These findings are consistent with a defect of high energy phosphate mitochondrial metabolism in thyroid dysfunction.     

非均匀采样干涉数据光谱反演技术研究

干涉光谱成像仪获取的干涉数据是一种中间数据,需要进行光谱反演才能够得到目标光谱数据,傅里叶变换方法是常规的光谱反演方法。由于干涉数据中存在非均匀采样问题,若忽略光谱混叠,直接采用快速傅里叶变换会导致反演光谱的失真,难以满足实时处理需求。针对非均匀采样干涉数据的光谱反演需求,将插值及非均匀快速傅里叶变换(NUFFT)方法应用到光谱反演处理中,对过采样及部分欠采样情况下的非均匀采样干涉数据,提出了相应的光谱反演方法,并分析了方法的适用性。最后对过采样及部分欠采样情况下的光谱反演方法进行计算机仿真,过采样情况下采用NUFFT方法反演光谱的精度要明显高于插值方法,而部分欠采样情况下插值方法反演光谱的精度要明显高于NUFFT方法,并对欠采样造成的光谱混叠有一定的修正,验证了方法的有效性。     

SUPERCONVERGENCE ANALYSIS OF THE POLYNOMIAL PRESERVING RECOVERY FOR ELLIPTIC PROBLEMS WITH ROBIN BOUNDARY CONDITIONS

We analyze the superconvergence property of the linear finite element method based on the polynomial preserving recovery(PPR)for Robin boundary elliptic problems on triangulartions.First,we improve the convergence rate between the finite element solution and the linear interpolation under the H1-norm by introducing a class of meshes satisfying the Condition(α,σ,μ).Then we prove the superconvergence of the recovered gradients post-processed by PPR and define an asymptotically exact a posteriori error estimator.Finally,numerical tests are provided to verify the theoretical findings.     

PIECEWISE SPARSE RECOVERY VIA PIECEWISE INVERSE SCALE SPACE ALGORITHM WITH DELETION RULE

In some applications, there are signals with piecewise structure to be recovered. In this paper, we propose a piecewise_ISS (P_ISS) method which aims to preserve the piecewise sparse structure (or the small-scaled entries) of piecewise signals. In order to avoid selecting redundant false small-scaled elements, we also implement the piecewise_ISS algorithm in parallel and distributed manners equipped with a deletion rule. Numerical experiments indicate that compared with aISS, the P_ISS algorithm is more effective and robust for piecewise sparse recovery.     

基于风险厌恶零售商的双渠道闭环供应链运作策略

在对称信息框架下,针对零售商存在风险厌恶特性的双渠道闭环供应链,基于Stackelberg博弈理论探讨了不同的回收再制造模式下(制造商不回收再制造、制造商独立回收再制造、制造商依托零售商回收再制造)供应链各主体的运作决策。研究发现,在不同回收再制造模式下,制造商与风险厌恶的零售商合作能够获得更多收益。市场波动的增大对供应链各主体并非始终是有害的,随着市场波动的增大,带有风险厌恶的零售商收益不断减小,而风险中性的双渠道制造商收益逐步增大,零售商风险厌恶特性为制造商带来了更多的收益。制造商独立回收再制造以及通过零售商进行回收再制造时,双渠道产品定价均低于不进行任何回收再制造模式下的定价,双渠道需求和利润均高于不进行任何回收再制造模式下的渠道需求和利润,即回收再制造模式不仅为下游顾客带来了更多的消费实惠,同时也为供应链各主体带来了更多的收益。     

应用模糊综合评判方法优选非均质油藏调剖井和层

通过注水保持油层压力的方法开发非均质油藏,不可避免会造成油井含水或部分油井水淹.注入水窜入油井时,不管低渗透层是否受到开发,大部分注入水进入到高渗透层,因而使低渗透层在开发过程中采油速度很慢,或是基本未投入生产.为了降低产水量,保持或提高采油量,必须对已开发的非均质油藏实施相应的堵水调剖措施,防止注入水沿高渗透带水锥突进到油井,影响水驱采收率.本文建立了应用模糊综合评判方法优选非均质油藏调剖井和层的数学模型,通过实例分析表明,运用综合评判方法优选调剖井和层是一种客观有效的方法,该评价方法计算方便,易于操作和推广.     

High Pressure High Temperature Dynamic Rheometry of Sulfonated Polyacrylamide Solutions in Electrolyte Media as Used for Oil Recovery Applications

Sulfonated polyacrylamide (SPAA) solutions were prepared and the effects of pressure, polymer concentration, and water temperature, pH and salinity on their rheological behavior were investigated using a concentric cylinder dynamic rheometer equipped with a high pressure cell. According to the rheological flow curves the shear stress of SPAA solutions increased less than in proportion to their shear rates; that is, a shear thinning effect occurred. For polymer solutions containing 15,000 ppm of SPAA, shear viscosity, and stress were nearly insensitive to pressure. However, the shear viscosity and stress of SPAA solutions were affected by temperature and this effect was more evident at lower pressure. The flow curves indicated the shear viscosity and stress of the samples increased with increasing SPAA concentration and pH of the water, but were decreased with increasing water salinity and temperature.