毛细管凝胶电泳的激光光热在线检测

近年来,毛细管电泳已成为一种高效快速的微化学分离分析新技术,在无机、有机及生物化学分析中得到了广泛的应用.但毛细管的内径小,进样量在pg～ng级,给样品的检测带来新的困难.因此,发展高灵敏度的显微在线检测器,已成为毛细管电泳技术发展中的重要环节.基于各种原理的检测器研究已有报道~[1].Dovichi等曾用热透镜技术在线检测了乙腈/     

一类新的多功能构筑基块-多吡唑化合物的设计合成

The design and synthesis of novel polypyrazolyl aromatic bridging ligands 1,3,5-tris（3,5-dimethyl-1-pyrazol-4-yl）benzene and 2,4,6-tris（4-（3,5-dimethyl-^1H-pyrazol-4-yl）phenyl）-1,3,5-triazine from simple starting materials mesitylene and p-tolunitrile were reported.     

Trace enrichment and HPLC analysis of polycyclic aromatic hydrocarbons in environmental samples,using solid phase extraction in connection with UV/VIS diode-array and fluorescence detection

Summary Both the separation of polycyclic aromatic hydrocarbons (PAH's) on tailor made HPLC phases, and their chromatographic enrichment and quantitative determination are of great interest. Because of the generally low concentrations of PAH's in the environment, and the low limiting values in German legislation for drinking water, methods for selective enrichment and sensitive detection are indispensable. On account of their relatively high amounts in soil, the determination of PAH's is valuable for the assessment of the potential danger to ground water by waste materials. These requirements can be fulfilled by used of solid phase extraction on enrichment columns, and fluorescence or UV/VIS diode array detection. For the detection of PAH's in the picogram range, the wavelengths for excitation and emission were time programmed over the chromatogram. With this feature, it is possible to detect all the individual compounds at the highest sensitivity, over the entire analysis.     

由二硫代肼基甲酸酯衍生的铜(Ⅱ)的希夫碱配合物的合成,结构和抑菌活性的研究

从S—苄基和S—甲基二硫代肼基甲酸酯衍生出的两种亚苄基丙酮希夫碱为配体,合成了铜(Ⅱ)配合物。经化学分析、磁性、红外、电子和EPR光谱的研究表明配合物有Cu(TSB)_2的形式,其中配体为双齿单价阴离子。在固态时,两种配合物分别为平面正方形和平面菱形结构。在溶液中,两者都为平面正方形。由EPR光谱分析得到的磁参数和键参数的值表明配合物中的键有较高的共价特征。对四种细菌的抑菌试验发现配体及其配合物均有抑菌活性,其中配合物的活性较高并研究了介质,pH对抑菌作用的影响。     

毛细管电泳安培检测扑热息痛及其水解物

研究了各种电化学预处理条件下碳纤维电极对扑热息痛及水解物的电化学行为。确定该体系最佳预处理条件为０．２Ｖ电位下阳极化１ｍｉｎ，再于－２．０Ｖ下阴极化１０ｓ。预处理后的碳纤维伏安响应得到明显提高。运用最佳条件并在支持电解中加入添加剂后，扑热息痛及其水解物在毛细管电泳上获得很好的分离和检测。其中扑热息痛的检测下限为２．７８ｐｇ；对氨基酚为１．８４ｐｇ。     

3-N-丙酰基-2-芳基-5-(2-碘苯基)-1,3,4-噁唑啉类化合物的合成

2-碘苯甲酰肼与芳醛反应得到相应的酰腙(1),1再与丙酸酐脱水环化合成了3-N-丙酰基-2-芳基-5-(2-碘苯基)-1,3,4-噁唑啉类化合物,其结构经1H NMR,IR,MS和元素分析表征。     

石英芯片电泳分离检测尿中蛋白的研究

通过对缓冲体系、缓冲液浓度、酸度、乳酸钙浓度、乙胺浓度、电泳电压和进样时间的优化选择，用石英芯片电泳一紫外检测法分离了纯人白蛋白和人运铁蛋白；在75mmol／L硼酸盐（pH10．55）（含0．8mmol／L乳酸钙、1％（φ）乙胺）运行缓冲液中，上述两组分在3min内完全分离；纯人白蛋白和人运铁蛋白的线性范围分别为1．0～15．0g/L和1．0～10．0g／L；检出限（S／N=3）均为0．5g／L，应用于临床尿蛋白分离测定，并与Helena琼脂糖凝胶电泳仪电泳结果进行比较，获得一致结果。     

On-chip potential gradient detection with a portable capillary electrophoresis system

A portable chip-CE system with potential gradient detection (PGD) was developed and applied to the determinations of alkali metals and alkaloids. The separation efficiency appeared to be satisfactory and nonaqueous capillary electrophoresis (NACE) proved to be applicable to PGD or conductivity detection. The power supplies, separation and detection were built on a device of 3 kg in weight. A branch channel near the end of the separation channel was designed to perform PGD and make the application of relatively high field strength possible. The study is the first report on the application of PGD on the microchip platform. The design of the chip-CE system shows several advantages, such as simplicity, miniaturization and wide applicability.     

对NO生理作用的新认识及其电化学实时检测

本文综述了近年来学术界对ＮＯ生理作用的新认识，并介绍了现场实时检测生物活体中释放的ＮＯ浓度的电化学方法．