运动对去卵巢大鼠骨元素代谢的影响

用测定骨元素含量的方法，分析了运动对正常大鼠和切除双侧卵巢后的大鼠骨元素代谢的影响。将健康4个月龄雌性SD大鼠44只随机分成5组：（1）非去势非运动组；（2）非去势＋运动组；（3）假去势非运动组；（4）去势非运动组；（5）去势＋运动组。运动组用大鼠专用跑台中等运动强度训练，持续10周。结果表明，去卵巢大鼠骨Ca,Mg,S,Mn,Zn等含量显著降低，P含量显著增加。运动训练可使去卵巢大鼠降低的骨Ca,Mg,S,Mn,Zn等含量显著回升，骨P含量显著回降。提示中等强度运动训练可对抗由于去卵巢所引起的骨元素代谢紊乱。     

粘度法研究高分子溶液行为的实验改进(Ⅱ)

高分子在粘度计毛细管管壁上的吸附不仅会导致毛细管有效管径减小，而且可以导致毛细管界面性质发生显著改变，具体表现为测定高分子溶液流过时间t之前和之后纯溶剂的流过时间t0和t0′与高分子溶液流过时间t对浓度作图外推到浓度为零时的值t0^*并不一致。不同温度时聚乙烯吡咯烷酮（PVP）水溶液粘度测定结果表明，当吸附讷发子显著改变了毛细管界面性质时，需要将高分子溶液粘度测定方法由t/t0′改为t/t0^*。经过改进的粘度测定方法不仅更加普适合理，而且更加简单有效。     

计算金属间化合物热力学性质的新方法

用一简单的相关函数式对金属间化合物的热力学性质进行拟合，提出了一种计算金属间化合物热力学性质的新方法，并用此方法计算了二元金属间化合物的常温热容和熵。计算结果表明，该方法简单，且能满足一定的计算精度，有一定的实用价值。     

Expeditious synthesis of porphyrin-cobaltacarborane conjugates

Two porphyrin-cobaltacarborane conjugates and were prepared in high yields via a nucleophilic ring-opening reaction of . These novel boron-rich and fluorescent compounds have potential application as boron delivery agents for the boron neutron capture therapy of tumors.     

Novel expanded porphyrinoids with multiple-inner-ring-fusion and/or tunable aromaticity

Novel expanded porphyrinoids with advanced structure features have a wide range of benefits (such as multi-metal coordination and facile tunable aromaticity) not offered by their normal porphyrin analogues. Considering research efforts have been devoted to address their limited synthetic accessibility issue. This review highlights some of these recent synthetic progresses towards these novel expanded porphyrinoids.     

Phase behaviour and microstructures of microemulsions (I)

By the orthogonal design, the optimal formation conditions for the middle-phase microemulsions in the system dioctadecyldimethylammonium chloride (DODMAC)/ sodium dodecyl sulfate (SDS)/n-butanol/n-hep-tane/brine were obtained as follows: W_DODMAC: W_SDS = 1:4-1:5,C _π-butanol (%) = 11.0-12.0, andC _NaCl (%) = 3.25 Investigations have been made on the effects of the concentrations of NaCl and n-butanol (l.0%-14.0%), the ratios ofWDODMAC: to W_SDS, and the kinds of alcohols (n-propanol, n-butanol, and n-pentanol) on the formation, the phase behaviour, the ultralow interfacial tensions, the optimal salinity (S^*), and the length of salinity (δS). Some rules and data were worked out about the formation and characteristics of the middle-phase microemulsions. The mi-crostructures of the middle-phase microemulsions were also studied by using FT-IR, ESR, and freeze fracture electron microscopy techniques. The results from the three methods show that the microstructures of the middlephase mi-croemulsions undergo the change from O/W to bicontinuous (B.C.) and to W/O. The distribution rule of the orga-nized molecule assemblies in the middle-phase microemulsions is conducible to constructing the model of microemulsion systems, to recognizing the microstructures of the middle-phase microemulsions, and to setting forth the relationship between the microstructures and macro-properties of rnicroemulsions. Project supported by the Niltional Natural Science Foundation of China and the Chinese Postdoctoral Science Foundation.     

微波辅助提取-GC/MS联用分析苍耳子中油脂成分的研究

采用微波辅助提取-GC/MS联用分析苍耳子中的油脂成分.以V(环已烷):V(丙酮)=1:1为提取溶剂,优化了微波辅助提取的条件.以正二十烷为内标,鉴定出了13种油脂成分,各组分相对保留时间的相对标准偏差(RSD)小于0.15%,相对峰面积的RSD＜3.0%.对不同批次以及不同产地的苍耳子样品进行了对比研究.该方法可用于苍耳子的鉴定和质量控制.     

Fabrication of lead titanate single crystalline nanowires by hydrothermal method and their characterization

Single crystalline nanowires of lead titanate (PbTiO₃) were fabricated by hydrothermal method at 200°C using lead acetate and n-tetrabutyl titanate as starting materials, where sodium hydroxide was served as a mineralizer. Crystalline phases, microstructure and optical properties of PbTiO₃ nanowires were investigated. The PbTiO₃ nanowires were uniform and continuous along the long axis, and were composed of single crystalline PbTiO₃ with a tetragonal perovskite structure. The diameter of a single nanowire was around 12 nm and the length reached up to 3 μm. The chemical composition of the samples and the valence states of elements were determined by X-ray photoelectron spectroscopy (XPS). The ultraviolet/visible absorption spectroscopic investigation suggested that the absorption edge of optical transition of the first excitonic state occurred at around 320 nm. A blue-green light emission peaking at about 471 nm (2.63 eV) is observed at room temperature, and the intensity of this emission increased with increasing excitation wavelength. Oxygen vacancies are responsible for the light emission of PbTiO₃ nanowires.     

A molecular docking model of SARS-CoV S1 protein in complex with its receptor, human ACE2

The exact residues within severe acute respiratory syndrome coronavirus (SARS-CoV) S1 protein and its receptor, human ACE2, involved in their interaction still remain largely undetermined. Identification of exact amino acid residues that are crucial for the interaction of S1 with ACE2 could provide working hypotheses for experimental studies and might be helpful for the development of antiviral inhibitor. In this paper, a molecular docking model of SARS-CoV S1 protein in complex with human ACE2 was constructed. The interacting residue pairs within this complex model and their contact types were also identified. Our model, supported by significant biochemical evidence, suggested receptor-binding residues were concentrated in two segments of S1 protein. In contrast, the interfacial residues in ACE2, though close to each other in tertiary structure, were found to be widely scattered in the primary sequence. In particular, the S1 residue ARG453 and ACE2 residue LYS341 might be the key residues in the complex formation.     

Dexmedetomidine promotes the progression of hepatocellular carcinoma through hepatic stellate cell activation

Dexmedetomidine (DEX) is an anesthetic that is widely used in the clinic, and it has been reported to exhibit paradoxical effects in the progression of multiple solid tumors. In this study, we sought to explore the mechanism by which DEX regulates hepatocellular carcinoma (HCC) progression underlying liver fibrosis. We determined the effects of DEX on tumor progression in an orthotopic HCC mouse model of fibrotic liver. A coculture system and a subcutaneous xenograft model involving coimplantation of mouse hepatoma cells (H22) and primary activated hepatic stellate cells (aHSCs) were used to study the effects of DEX on HCC progression. We found that in the preclinical mouse model of liver fibrosis, DEX treatment significantly shortened median survival time and promoted tumor growth, intrahepatic metastasis and pulmonary metastasis. The DEX receptor (ADRA2A) was mainly expressed in aHSCs but was barely detected in HCC cells. DEX dramatically reinforced HCC malignant behaviors in the presence of aHSCs in both the coculture system and the coimplantation mouse model, but DEX alone exerted no significant effects on the malignancy of HCC. Mechanistically, DEX induced IL-6 secretion from aHSCs and promoted HCC progression via STAT3 activation. Our findings provide evidence that the clinical application of DEX may cause undesirable side effects in HCC patients with liver fibrosis.Subject terms: Cancer microenvironment, Cell growth