Studies on Aggregation Interaction between Reduced Denatured Egg White Lysozymes during Refolding Procedure in Urea Solution

The aggregation interaction between reduced-denatured egg white lysozymes during refolding procedure in urea solution was studied by means of reducing and non-reducing protein electrophoreses. Results of non-reducing sodium dodecyl sulphate polyacrylamide gel electrophoresis （SDS-PAGE） of the supernatant and aggregate precipitate formed in refolding process show that except being refolded to native egg white lysozymes, the reduced-denatured lysozymes can also form the aggregates with molecular weights （MW） being separately about 30.0 and 35.0 kD, while the reducing SDS-PAGE and the refolding results in the presence of sodium dodecyl sulphate show that these aggregates are formed chiefly through the misconnection of disulfide bonds between the reduced-denatured lysozymes, and the aggregate precipitates are formed through the non-covalent interactions between the aggregates with molecular weight being about 30.0 kD. From the results of electrophoresis and size-exclusion chromatographic analyses, it can be inferred that the aggregates with molecular weights being about 30.0 and 35.0 kD are bi-molecular and tri-molecular egg white lysozyme aggregates, respectively. And finally, a suggested refolding mechanism of reduced-denatured egg white lysozymes in urea solution was presented.     

水分子团簇分子力场方法的比较研究

采用传统水分子力场模型(SPC, TIPnP(n=3-5))和极化模型(POL3, AMOEBA, SPC-FQ, TIP4P-FQ)对水分子二聚体团簇性质进行了比较和研究. 以从头计算和实验数据为依据, 分析水分子在外场作用下体系的静电极化, 电荷转移和分子结构变化. 通过水分子二聚体结合能和各分解能量项评价极化静电势能在双分子结合能中的地位和作用, 以及各水分子力场的适用性. 通过水分子团簇多体相互作用能的计算,展示不同极化水分子力场定量计算极化能量的实际能力. 通过对力场模型结果的对比和分析, 为进一步发展极化力场模型, 并应用到其他体系提供借鉴和依据.     

Mixed-valent diruthenium diphosphonate with kagomé structure

This paper reports the first example of diruthenium phosphonate with kagomé structure, namely, [NH3(CH2)4NH3][Ru2(hedpH(0.5))2].2H2O (hedp = 1-hydroxyethylidenediphosphonate). The compound contains kagomé layers that are linked by very strong hydrogen bonds into a nanoscale kagomé structure. Ferromagnetic interactions are mediated between the paramagnetic diruthenium units.     

新显色剂8-[杯(4)芳烃偶氮]氨基喹啉的合成及其与金的显色反应

合成了一种新的杂环偶氮类显色试剂 8 [杯 ( 4 )芳烃偶氮 ]氨基喹啉(CAQ) 1。研究了该试剂与金的显色反应的适宜条件 ,在碱性介质中 ,在CTMAB存在下 ,试剂与金生成 1∶1稳定络合物 ,建立了测定金的光度法新体系 ,体系至少可稳定 8h ,λmax =640nm ,ε =2 .0 6× 1 0 5L·mol-1·cm-1,金含量在 0～ 2 2μg 2 5mL内符合比尔定律。方法已用于金矿石中微量金的测定。     

Synthesis of New Chiral C2-Symmetrical Bis(oxazoline )Compounds

New chiral C2-symmetrical bis(oxazoline)compounds bearing one isobutyl-,secbutyl-benzyl- or isopropyl-substituent at the 4-position have been prepared and characterized.     

流动注射—化学发光法测定苯甲醛

依据苯甲醛增强没食子酸－过氧化氢－氢氧化钠体系化学发光的性质，建立了苯甲醛的流动注射化学发光分析法。     

微分倒数示波计时电位法的研究—定量测试及滴定分析

首次利用微分倒数示波图定量测试微量金属离子并滴定分析药物，结果满意。     

植酸与稀土元素的固态配合物的研究

植酸（PllytiC。Cid）又称肌醇六磷酸酯（myo－iflOSitdhX。hSPhOSPh。ie）（简写为H12lHP］常以钙、镁复盐（菲汀Phntin）形式存在于植物中,尤其在谷类和植物的种子中含量较高［'j．植酸具有惊人的骛合能力,除碱金属外几乎能与所有金属离子生成沉淀,一些高价金属离于甚至在强酸性介质中也可定量沉淀［如Ti、Zr、Hf、Th、Ce（IV）和In、Sc等〕,这些性质已初用于Th'"的定量测定L'和SC'"的分离提取「",Gd'"与植酸的配合物被用作核磁共振成倍（MRI）的对比剂［'j．然而,对有关配合物的固态组成及性质研究尚无文献报道．本文…     

Pushing up the size limit of chalcogenide supertetrahedral clusters: two- and three-dimensional photoluminescent open frameworks from (Cu(5)In(30)S(54))(13-) clusters

Direct band gap copper indium chalcogenides are of great technological importance in part because of their high photovoltaic conversion efficiency. Covalent superlattices constructed from copper indium chalcogenide clusters are of particular interest because they may combine open framework architecture with semiconducting properties. Here two photoluminescent covalent superlattices built from core-shell type copper indium sulfide supertetrahedral clusters are reported. Each cluster consists of 35 metal cations and is so far the largest known supertetrahedral cluster with a metal-to-metal distance of 1.6 nm. In addition, this is the first example of supertetrahedral clusters in heterometallic copper indium chalcogenides. The preparation of these large clusters has narrowed down the size gap between colloidal nanoclusters and small supertetrahedral clusters and revealed new possibilities in the construction of nanoporous semiconducting superlattices with tunable pore size. Through the combination of metal ions with different oxidation states to provide both overall and local charge neutrality, an effective approach has been demonstrated in the rational synthesis of chalcogenide open framework materials with large and unprecedented supertetrahedral clusters.