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31.
用硒与尿素化合,新生的由杂环有机硒所组成的Zs-93硒剂治疗移植性S180肉瘤,有显著的抑瘤作用(P<0.01),其作用表现出一定的剂量效应,以5μg/只小鼠为最适,抑瘤率达60.76%,治疗组小鼠的T细胞、SOD活性均有增强。 相似文献
32.
Intracellular delivery of enzymes is essential for protein-based diagnostic and therapeutic applications. Protein-spherical nucleic acids (ProSNAs) defined by protein core and dense shell of oligonucleotides have been demonstrated as a promising vehicle-free enzyme delivery platform. In this work, we reported a crosslinking strategy to vastly improve both delivery efficiency and intracellular sensor performance of ProSNA. By assembling individual ProSNA with lactate oxidase (LOX) core into a nanoscale particle, termed as crosslinked SNA (X-SNA), the enzyme delivery efficiency increased up to 5–6 times higher. The LOX X-SNA was later demonstrated as a ratiometric probe for quantitative detection of lactate in living cells. More importantly, X-SNA probe showed significantly improved sensor performance with signal-to-noise ratio 4 times as high as ProSNA when detecting intracellular lactate.By crosslinking protein spherical nucleic acid (SNA) into a supramolecular architecture X-SNA, the intracellular enzyme delivery efficiency was significantly enhanced, showing 3–4 times higher signal-to-noise ratio in detecting intracellular lactate. 相似文献
33.
Ly A Bandong SL Tran NQ Sullivan KJ Milligan JR 《The journal of physical chemistry. B》2005,109(27):13368-13374
Guanine bases are the most easily oxidized sites in DNA. Electron-deficient guanine species are major intermediates produced in DNA by the direct effect of ionizing radiation (ionization of the DNA itself) because of preferential hole migration within DNA to guanine bases. By using thiocyanate ions to modify the indirect effect (ionization of the solvent), we are able to produce these single-electron-oxidized guanine radical species in dilute aqueous solutions of plasmid DNA where the direct effect is negligible. The guanyl radical species produce stable modified guanine products. They can be detected in the plasmid by converting them to strand breaks after incubation with a DNA repair enzyme. If a phenol is present during irradiation, the yield of modified guanines is decreased. The mechanism is reduction of the guanine radical species by the phenol. It is possible to derive a rate constant for the reaction of the phenol with the guanyl radical. The pH dependence shows that phenolate anions are more reactive than their conjugate acids, although the difference for guanyl radicals is smaller than with other single-electron-oxidizing agents. At physiological pH values, the reduction of a guanyl radical entails the transfer of a proton in addition to the electron. The relatively small dependence of the rate constant on the driving force implies that the electron cannot be transferred before the proton. These results emphasize the potential importance of acidic tyrosine residues and the intimate involvement of protons in DNA repair. 相似文献
34.
35.
Mai Thanh Thi Nguyen 《Tetrahedron letters》2004,45(46):8519-8522
A novel dimeric methanodibenzoxocinone, named neosappanone A (1), possessing a unique unprecedented novel carbon framework, has been isolated from the heartwood of Caesalpinia sappan L. of Vietnam, and its structure was elucidated on the basis of spectroscopic analysis. Neosappanone A (1) competitively inhibited xanthine oxidase in a concentration-dependent manner (IC50, 29.7 μM; Ki, 16.3 μM). 相似文献
36.
37.
Yi‐Hang Wen Xia Feng Yin‐Hua He You‐Zhao Lan Hong Sun 《Acta Crystallographica. Section C, Structural Chemistry》2007,63(11):m504-m506
In the title complex, [Zn(C12H6O4)(H2O)]n, a ZnII polymer based on naphthalene‐1,8‐dicarboxylate (1,8‐nap), the ZnII atoms adopt an elongated octahedral coordination geometry. A zigzag chain is formed by μ2‐aqua ligands and μ2‐carboxylate groups of the 1,8‐nap ligands. Adjacent parallel chains are further linked by 1,8‐nap ligands, forming a twisted two‐dimensional layer structure along the (100) plane. 相似文献
38.
Terephthalamide derivatives as mimetics of helical peptides: disruption of the Bcl-x(L)/Bak interaction 总被引:4,自引:0,他引:4
Yin H Lee GI Sedey KA Rodriguez JM Wang HG Sebti SM Hamilton AD 《Journal of the American Chemical Society》2005,127(15):5463-5468
A series of Bcl-x(L)/Bak antagonists, based on a terephthalamide scaffold, was designed to mimic the alpha-helical region of the Bak peptide. These molecules showed favorable in vitro activities in disrupting the Bcl-x(L)/Bak BH3 domain complex (terephthalamides 9 and 26, K(i) = 0.78 +/- 0.07 and 1.85 +/- 0.32 microM, respectively). Extensive structure-affinity studies demonstrated a correlation between the ability of terephthalamide derivatives to disrupt Bcl-x(L)/Bak complex formation and the size of variable side chains on these molecules. Treatment of human HEK293 cells with the terephthalamide derivative 26 resulted in disruption of the Bcl-x(L)/Bax interaction in whole cells with an IC(50) of 35.0 microM. Computational docking simulations and NMR experiments suggested that the binding cleft for the BH3 domain of the Bak peptide on the surface of Bcl-x(L) is the target area for these synthetic inhibitors. 相似文献
39.
Tran CD Grishko VI Challa S 《Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy》2005,62(1-3):38-41
A novel method has been developed for the sensitive and accurate determination of compositions of fullerene samples. The method is based on the synergistic use of spectrophotometric measurements and partial least square method. The method is not only simple, inexpensive and fast but also is non-destructive. Compositions of various fullerene samples including fullerite which is the precursor to C(60) and C(70), can be directly and non-destructively determined by this method without any time-consuming separation step as in the HPLC method or destruction as in the MS method. 相似文献
40.
原位沉析法制备磁性氧化铁羟基磷灰石/壳聚糖棒材 总被引:3,自引:0,他引:3
首先通过化学沉淀法制备磁性氧化铁羟基磷灰石(Fe3O4/HA),然后以壳聚糖(CS)为基体,利用原位沉析法将Fe3O4/HA与CS复合,制得磁性Fe3O4/HA/CS复合材料.经XRD、粒径分布和PPMS测试,结果表明了Fe3O4/HA复合物的生成.系统研究了磁性Fe3O4/HA/CS棒材力学性能的影响因素,最终确定Fe3O4与HA质量比为3∶17,磁性Fe3O4/HA与CS质量比为9∶91时,棒材的力学性能最优,弯曲强度可达到87.0 MPa,弯曲模量1.57 GPa. 相似文献