Tetrazole Photoclick Chemistry: Reinvestigating Its Suitability as a Bioorthogonal Reaction and Potential Applications

The bioorthogonality of tetrazole photoclick chemistry has been reassessed. Upon photolysis of a tetrazole, the highly reactive nitrile imine formed undergoes rapid nucleophilic reaction with a variety of nucleophiles present in a biological system, along with the expected cycloaddition with alkenes. The alternative use of the tetrazole photoclick reaction was thus explored: tetrazoles were incorporated into Bodipy and Acedan dyes, providing novel photo‐crosslinkers with one‐ and two‐photon fluorescence Turn‐ON properties that may be developed into protein‐detecting biosensors. Further introduction of these photo‐activatable, fluorogenic moieties into staurosporine resulted in the corresponding probes capable of photoinduced, no‐wash imaging of endogenous kinase activities in live mammalian cells.     

Single Nanoparticle Voltammetry: Contact Modulation of the Mediated Current

The cyclic voltammetric responses of individual palladium‐coated carbon nanotubes are reported. Upon impact—from the solution phase—with the electrified interface, the nanoparticles act as individual nanoelectrodes catalyzing the hydrogen‐oxidation reaction. At high overpotentials the current is shown to reach a quasi‐steady‐state diffusion limit, allowing determination of the tube length. The electrochemical response of the individual nanotubes also reveals the system to be modulated by the electrical contact between the electrode and carbon nanotube. This modulation presents itself as fluctuations in the recorded Faradaic current.     

Fluorescence and HPLC Detection of Hydroxyl Radical by a Rhodamine-Nitroxide Probe and its Application in Cell Imaging

A rhodamine nitroxide probe was designed to detect the hydroxyl radical (·OH), which presented high selectivity for ·OH over other reactive oxygen species (ROS) and linear fluorescence response to ·OH produced by Fenton reaction. The product was detected by HPLC-MS, indicating that the main product of the reaction was O-methylhydroxylamine and the product peak areas measured by HPLC-UV/vis and HPLC-FLD both enhanced proportionally with the increase of ·OH concentration. The application of the probe in biological system was explored to trace the production of ·OH in cells under oxidative stress condition induced by rotenone which can inhibit the mitochondria respiratory chain complex I and we found that appropriate rotenone may induce the normal human liver cells (L02) and human hepatoma cells (HepG2) to produce ·OH at different degrees.     

Enhanced photoluminescence of CdSe quantum dots by the coupling of Ag nanocube and Ag film

The coupling of local surface plasmon(LSP) of nanoparticle and surface plasmon(SP) mode produced by metal film can lead to the enhanced electromagnetic field, which has an important application in enhancing the fluorescence of quantum dots(QDs). Herein, the Ag nanocube and Ag film are used to enhance the fluorescence of CdSe QDs. The enhancement is found to relate to the sizes of the Ag nanocube and the thickness of the Ag film. Moreover, we also present the fluorescence enhancement caused by only SP. The result shows that the coupling between metal nanoparticles and metal film can realize larger field enhancement. Numerical simulation verifies that a nanocube can localize a strong electric field around its corner. All the results indicate that the fluorescence of QDs can be efficiently improved by optimizing the parameters of Ag film and Ag cubes.     

Laser-induced damage of the optical films prepared by electron beam evaporation and ion beam sputtering in vacuum

The laser-induced damage characteristics and adsorption effects of organic contamination molecules of two high reflectors (HR) prepared by electron beam evaporation (EB) and ion beam sputtering (IBS) method at 1064 nm is investigated in vacuum. It is found that EB films show the performance degradation of laser induced damages in vacuum while for IBS film, seems to have no this effect, in comparison with air environment. In addition, EB coatings also have the strong affinity with organic contamination molecules, in contrast of IBS films. The results reveal that ion beam sputtering (IBS) method seem to be one of the favorite film deposition techniques of the optical films used in vacuum and space environments.     

MnO2/graphite electrodeposited under supergravity field for supercapacitors and its electrochemical properties

MnO₂/graphite electrode material is successfully synthesized by electrodeposition under supergravity field from manganese acetate and graphite suspending solution. X-ray diffraction and field emission scanning electron microscopy show that the obtained composite is γ-MnO₂/graphite. The process of depositing the MnO₂/graphite was shown by the schematic illustration. Galvanostatic charge/discharge and cyclic voltammograms tests are applied to investigate electrochemical performances of the composite electrodes prepared under supergravity fields. MnO₂/graphite synthesized under supergravity field exhibits good discharge capacitance and the specific capacitance is 367.77 F g^?1 at current density of 0.5 A g^?1. It is found that supergravity field has effects on the electrochemical performances of MnO₂/graphite material.     

全景视觉系统发展与应用

全景视觉系统具有视场范围大的特点,并且系统结构种类繁多,各有特色,已广泛应用在机器人导航、空间探测、视频监控、虚拟现实、环境感知技术等民用或军用领域;全景视觉系统由全景视觉光学成像系统、图像传感器、图像处理系统和输出图像显示等组成;文章主要从全景视觉成像角度对全景视觉系统进行分类,并详细介绍了每一类的全景成像原理、发展现状和应用情况,最后对每种类型全景视觉系统的优缺点给予分析总结。     

一种新型的渐变脊径向波导空间功率合成器设计

给出了一种新型的波导渐变脊的设计方法及其在波导空间功率合成器中的应用,该设计方法采用新型复合函数渐变曲线代替了传统的单一指数函数或三角函数渐变曲线,通过优化渐变脊的结构可以达到对频带内引起的谐振进行抑制的目的,并能降低功率合成器中的通路损耗;将该新型波导渐变脊结构用于30~40 GHz的波导空间功率合成器,经测试得到,由渐变脊组成的合成器部分在整个频段内插入损耗不足0.8 dB,整个无源合成器结构的输入端口驻波小于1.35,满足了合成器的使用要求。     

基于符号函数的注入反馈式蔡氏电路同步控制

蔡氏电路的状态变量不易引出,一般的同步控制方法难以硬件电路实现.针对此类含有电感、电容储能元件的混沌电路,提出一种注入反馈式混沌同步电路设计方法,通过引出状态变量,并在状态变量微分量未知的情况下对系统实施混沌控制.利用该方法,对运动行为不同的两个蔡氏系统,实现了基于符号函数的非线性投影同步控制研究,电路实验结果验证了该方法的可行性和有效性.