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991.
The complexation of lysozyme and sodium (sulfamate carboxylate) isoprene/ethylene oxide (SCIEO) at pH = 7.4 and the release of lysozyme from the complexes in the presence of NaCl were investigated. Through electrostatic and hydrophobic interactions, lysozyme and SCIEO form stable complex nanoparticles. The complexation partially disturbs the structure of lysozyme. Some of the hydrophobic residues of lysozyme are exposed to bind with SCIEO. The complexation leads to loss of most of the lysozyme activity. In the presence of NaCl, lysozyme can be released from the complexes. The released lysozyme molecules recover their native structure and activity completely. In the condition of physiological pH and ionic strength, a sustained and extended release of lysozyme was achieved.
992.
Qiong Liu Xueying Liang Decong Hu Ping Chen Jing Tian Hong Zhang 《Frontiers of Chemistry in China》2010,5(1):88-98
Selenium (Se) is an essential trace element in vivo involved in the defense against oxidative stress. Se deficiency is associated with many human diseases. The bioactivity of
Se is dose- and species-dependent. Silkworm pupa has been reported to accumulate Se mainly in proteins. Thus the characterization
of major Se-containing proteins is very important in the application of Se-rich silkworm pupas in food and drugs. In this
study, crude proteins were extracted from Se-rich silkworm pupas, followed by DEAE-Sepharose and Sephedex G-75 chromatography.
Se content was measured after each step to determine the highest Se-containing fraction for the next step of separation. The
proteins obtained were analyzed using SDS-PAGE, followed by in-gel digestion with trypsin, and were characterized by MALDI-TOF
MS and ESI-MS/MS. These data showed two proteins mainly accumulated Se in the silkworm pupas. Those two proteins were proven
by mass spectrometry to be arylphorin and sex-specific storage-protein 2 precursor (SP-2), respectively. Both of them belong
to the storage proteins of amino acids during metamorphosis and the non-feeding pupal stage. The results suggest that Se could
be enriched by storage proteins and be supplied to silkworm pupas in accompany with amino acids for the synthesis of new Se-containing
proteins and peptides. 相似文献
993.
Yan Wu Gui‐Ge Hou Jian‐Ping Ma Yu‐Bin Dong 《Acta Crystallographica. Section C, Structural Chemistry》2010,66(6):m163-m165
The title molecular complex, [CoCl2(C22H18N6O)], features a novel 18‐membered Co‐containing metallocycle. The CoII atom lies in a fairly regular tetrahedral geometry defined by two imidazole N‐atom donors from one 2,5‐bis[3‐(1H‐1,3‐imidazol‐1‐ylmethyl)phenyl]‐1,3,4‐oxadiazole (L) ligand and two chloride anions. The coordinating orientation of the L ligand plays an important role in constructing the metallocycle complex. The complexes form a three‐dimensional supramolecular assembly via nonclassical C—H...Cl and C—H...N hydrogen bonds and π–π interactions. 相似文献
994.
Yu‐Bing Lu Ping Yang Wen‐Na Huang Yan‐Na Yang Jian‐Zhong Wu 《Acta Crystallographica. Section C, Structural Chemistry》2010,66(12):o596-o599
Both title compounds are derivatives of salicylic acid. 5‐Formylsalicylic acid (systematic name: 5‐formyl‐2‐hydroxybenzoic acid), C8H6O4, possesses three good hydrogen‐bond donors and/or acceptors coplanar with their attached benzene ring and abides very well by Etter's hydrogen‐bond rules. Intermolecular O—H...O and some weak C—H...O hydrogen bonds link the molecules into a planar sheet. Reaction of this acid and o‐phenylenediamine in refluxing ethanol produced in high yield the new zwitterionic compound 5‐(benzimidazolium‐2‐yl)salicylate [systematic name: 5‐(1H‐benzimidazol‐3‐ium‐2‐yl)‐2‐hydroxybenzoate], C14H10N2O3. Each imidazolium N—H group and its adjacent salicyl C—H group chelate one carboxylate O atom via hydrogen bonds, forming seven‐membered rings. As a result of steric hindrance, the planes of the molecules within these pairs of hydrogen‐bonded molecules are inclined to one another by ∼74°. There are also π–π stacking interactions between the parallel planes of the imidazole ring and the benzene ring of the salicyl component of the adjacent molecule on one side and the benzimidazolium component of the molecule on the other side. 相似文献
995.
溶胶-凝胶流动相异型直接甲醇燃料电池性能研究 总被引:1,自引:0,他引:1
以掺杂石墨粉的中间相碳微球(MCMB/G)烧结管为阴极支撑体,采用浸涂工艺分别制备了扩散层和催化层并在其外表面包裹Nafion膜,制得管状异型阴极并组装成异型直接甲醇燃料电池;采用溶胶-凝胶法制备了适用于直接甲醇燃料电池的溶胶-凝胶流动相。研究了溶胶-凝胶流动相异型直接甲醇燃料电池的阻抗,考察了阴极支撑体壁厚、阴极扩散层载量、实验温度和溶胶黏度等对电池极化性能的影响。结果表明,异型电池阻抗比传统平板电池大,但活化后电池阻抗明显下降;较低的溶胶黏度和较高的工作温度有利于提高电池性能;支撑体壁厚为1.3 mm、扩散层载量为3.5 mg/cm2时的电极性能最优。 相似文献
996.
离子液体作用下微波辐照生物质快速热解制备生物质油(英文) 总被引:2,自引:0,他引:2
以离子液体1-丁基-3-甲基咪唑氯([Bmim]Cl)和1-丁基-3甲基咪唑四氟化硼([Bmim]BF4)为催化剂,在微波加热作用下,研究了稻草和锯屑的热解。微波加热20 min,稻草和锯屑的生物油产率分别为38%和34%。考察了微波加热时间、微波功率和离子液体用量对生物质油产率的影响。当以相同的离子液体为催化剂时,稻草微波热解得到的生物质油产率大于锯屑的。生物油成分主要有糠醛、醋酸和1-羟基-2-丁酮等,其含量主要取决于生物质原料和加入的离子液体的类型。 相似文献
997.
998.
小批量制备质谱纯鲨鱼肝铁蛋白(Liver Ferritin of Sphyrna zygaena,SZLF)。在弱酸介质(pH1.0)中,天然电泳结果显示,SZLF蛋白质亚基20 min后开始解离。选用透射电子显微镜跟踪SZLF亚基解离与重组装全过程和蛋白壳与铁核尺寸变化,发现SZLF在亚基酸解离过程中,随着pH值的降低,铁核和蛋白壳的尺寸呈现相同的变化趋势,这种变化趋势可能与铁核内层铁的释放和蛋白壳的解离与去折叠有关。SZLF蛋白壳的重组装过程则是一个快速过程,并且是由松散熔球态向紧密态转变的过程。SZLF由单类型亚基组成,而马脾铁蛋白(Horse Spleen Ferritin,HSF)由H和L两种亚基类型组成。在基质pH3.0条件和激光辅助下,混合HSF和SZLF仍然可释放各自的亚基且形成准亚基离子,供基质辅助激光解析电离飞行时间质谱分析,说明此时SZLF的亚基间相互作用强度减弱但并没有去折叠。TEM技术在铁蛋白解离和重组装过程中的应用,为进一步研究铁蛋白纳米包装的过程和机理提供新颖的、可行的和更加直观的研究手段。 相似文献
999.
Sample preparation holds an important place in MS-based proteome analysis. For effective proteolysis and MS analysis, it is essential to eliminate the interferences while extracting the analytes of interest from complex mixtures. To address this, herein we describe a new dried polyacrylamide gel absorption method. In this method, the protein sample prepared using high concentration of SDS was directly and completely absorbed by vacuum-dried polyacrylamide gel, and then the interfering substances including SDS and some other salts were efficiently removed by in-gel washing steps while retaining the denatured proteins in the gel, thus offering a clean environment amenable to downstream buffer exchange, proteolytic digestion and digest recovery, etc. In combination with in-gel digestion and LC-MS/MS, the newly developed method was applied to the proteome analyses of membrane-enriched fraction and whole tissue homogenate. It was demonstrated that the method is suitable for the analysis of a complex biological sample and can be widely used for sample cleanup in shotgun proteome analyses. 相似文献
1000.
小批量制备了电泳纯鲨鱼肝铁蛋白(Liver ferritin ofSphyrna zygaena,SZLF),对SZLF的结构与功能进行了研究.在pH=2.0~10.0条件下,选用圆二色性(Circular dichroism,CD)光谱技术研究了SZLF和脱铁核SZLF(apoSZLF)二级结构转换的基本特征和变化趋势,揭示了SZLF蛋白壳的亚基稳定性、相互作用强度和去折叠现象.利用酸碱中和方法,解离SZLF蛋白壳亚基,并再次构建成完整SZLF.用紫外-可见分光光度法和荧光光度法研究了构建纳米吡啰红G核-铁蛋白的途径.定量分析结果表明,每分子apoSZLF可捕获12分子吡啰红G于蛋白壳内,构建纳米吡啰红G核-铁蛋白.分析了apoSZLF直接捕获和释放吡啰红G的途径与速率,为后续构建高存量纳米顺铂核-铁蛋白药物载体提供了可行性技术. 相似文献