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61.
This paper focuses on extracting depth information with an aperture-rotating camera. Compared with previous work, the proposed method is applicable for arbitrary rotation angle by making use of pixel registration to get pixel shift angle, and calculating the pixel shift along this angle. Besides, consumption time for shift angle is reduced by means of sampling. Compared with point-by-point method, the proposed method reduces the computation time from about 1 h to 1 min for a 15-megapixel image. Experimental results of a scene named “bottles” show that the depth value has a margin of error of 5%. Proposed method makes it possible to extract depth information with a single camera and expands the application of DSLR camera. 相似文献
62.
Sun D Zhang R Yuan F Liu D Zhou Y Liu J 《Dalton transactions (Cambridge, England : 2003)》2012,41(6):1734-1741
Two arene ruthenium complexes [Ru(η(6)-C(6)H(6))(p-MOPIP)Cl](+)1 and [Ru(η(6)-C(6)H(6))(p-CFPIP)Cl](+)2, where p-MOPIP = 2-(4-methoxyphenyl)-imidazo[4,5f][1,10] phenanthroline and p-CFPIP = 2-(4-trifluoromethylphenyl)-imidazo[4,5f][1,10] phenanthroline, were prepared and the interactions of these compounds with DNA oligomers 5'-G3(T2AG3)3-3'(HTG21) have been studied by UV-vis and circular dichroism (CD) spectroscopy, gel mobility shift assay, fluorescence resonance energy transfer (FRET) melting assay, polymerase chain reaction (PCR) stop assay and telomeric repeat amplification protocol (TRAP) assay. The results show that both complexes can induce the stabilization of quadruplex DNA but complex 1 is a better G-quadruplex binder than complex 2. The two ruthenium complexes tested led to an inhibition of the enzyme telomerase and complex 1 was the significantly better inhibitor. A novel visual method has been developed for making a distinction between G-quadruplex DNA and double DNA by our Ru complexes binding hemin to form the hemin-G-quadruplex DNAzyme. Furthermore, in vitro cytotoxicity studies showed complex 1 exhibited quite potent antitumor activities and the greatest inhibitory selectivity against cancer cell lines. 相似文献
63.
M Collot C Loukou AV Yakovlev CD Wilms D Li A Evrard A Zamaleeva L Bourdieu JF Léger N Ropert J Eilers M Oheim A Feltz JM Mallet 《Journal of the American Chemical Society》2012,134(36):14923-14931
We designed Calcium Rubies, a family of functionalizable BAPTA-based red-fluorescent calcium (Ca(2+)) indicators as new tools for biological Ca(2+) imaging. The specificity of this Ca(2+)-indicator family is its side arm, attached on the ethylene glycol bridge that allows coupling the indicator to various groups while leaving open the possibility of aromatic substitutions on the BAPTA core for tuning the Ca(2+)-binding affinity. Using this possibility we now synthesize and characterize three different CaRubies with affinities between 3 and 22 μM. Their long excitation and emission wavelengths (peaks at 586/604 nm) allow their use in otherwise challenging multicolor experiments, e.g., when combining Ca(2+) uncaging or optogenetic stimulation with Ca(2+) imaging in cells expressing fluorescent proteins. We illustrate this capacity by the detection of Ca(2+) transients evoked by blue light in cultured astrocytes expressing CatCh, a light-sensitive Ca(2+)-translocating channelrhodopsin linked to yellow fluorescent protein. Using time-correlated single-photon counting, we measured fluorescence lifetimes for all CaRubies and demonstrate a 10-fold increase in the average lifetime upon Ca(2+) chelation. Since only the fluorescence quantum yield but not the absorbance of the CaRubies is Ca(2+)-dependent, calibrated two-photon fluorescence excitation measurements of absolute Ca(2+) concentrations are feasible. 相似文献
64.
Dr. Dongdong Sun Dr. Yanan Liu Du Liu Rong Zhang Xicheng Yang Prof. Jie Liu 《Chemistry (Weinheim an der Bergstrasse, Germany)》2012,18(14):4285-4295
Telomerase inhibition is an attractive strategy for cancer chemotherapy. In the current study, we have synthesized and characterized two chiral ruthenium(II) complexes, namely, Λ‐[Ru(phen)2(p‐MOPIP)]2+ and Δ‐[Ru(phen)2(p‐MOPIP)]2+, where phen is 1,10‐phenanthroline and p‐MOPIP is 2‐(4‐methoxyphenyl)‐imidazo[4,5f][1,10]phenanthroline. The chiral selectivity of the compounds and their ability to discriminate quadruplex DNA were investigated by using UV/Vis, fluorescence spectroscopy, circular dichroism spectroscopy, fluorescence resonance energy transfer melting assay, polymerase chain reaction stop assay and telomerase repeat amplification protocol. The results indicate that the two chiral compounds could induce and stabilize the formation of antiparallel G‐quadruplexes of telomeric DNA in the presence or absence of metal cations. We report the remarkable ability of the two complexes Λ‐[Ru(phen)2(p‐MOPIP)]2+ and Δ‐[Ru(phen)2(p‐MOPIP)]2+ to stabilize selectively G‐quadruplex DNA; the former is a better G‐quadruplex binder than the latter. The anticancer activities of these complexes were evaluated by using the MTT assay. Interestingly, the antiproliferative activity of Λ‐[Ru(phen)2(p‐MOPIP)]2+ was higher than that of Δ‐[Ru(phen)2(p‐MOPIP)]2+, and Λ‐[Ru(phen)2(p‐MOPIP)]2+ showed a significant antitumor activity in HepG2 cells. The status of the nuclei in Λ/Δ‐[Ru(phen)2(p‐MOPIP)]2+‐treated HepG2 cells was investigated by using real‐time living cell microscopy to determine the effects of Λ/Δ‐[Ru(phen)2(p‐MOPIP)]2+ on intracellular accumulation. The results show that Λ/Δ‐[Ru(phen)2(p‐MOPIP)]2+ can be taken up by HepG2 cells and can enter into the cytoplasm as well as accumulate in the nuclei; this suggests that the nuclei were the cellular targets of Λ/Δ‐[Ru(phen)2(p‐MOPIP)]2+. 相似文献
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67.
介绍了一种可以完成脉宽、幅值、频率可调、十路脉冲输出且延时可调功能的程控脉冲发生器。硬件主要包括主电源和辅助电源、功率放大电路、控制系统处理器、数字键盘和液晶显示屏。该脉冲发生器输出脉冲宽度可在1~30 s间调节,脉冲幅值在1~15 V间调节,输出脉冲频率范围为1 Hz~30 kHz,十路脉冲输出中每路脉冲之间可以在0~1 ms范围内精确调节。该脉冲发生器可为多个脉冲源的并联运行提供延时触发,为多个绝缘栅双极型晶体管(IGBT)开关串联提供同步触发。 相似文献
68.
Hu Z Liang D Zhao J Huang J Zhu Q 《Chemical communications (Cambridge, England)》2012,48(59):7371-7373
A base-controlled synthesis of 2-substituted secondary and tertiary 1H-indole-3-carboxamides through PdCl(2)-catalyzed cyclization of o-alkynyltrifluoroacetanilides followed by isocyanide insertion has been developed. The reaction proceeds smoothly at ambient temperature using O(2) in air as the sole oxidant of the palladium catalyst. 相似文献
69.
一种抑制杂交元零能模式的假设应力场方法 总被引:1,自引:0,他引:1
基于杂交元位移场直接导出可以表示单元任意变形的简单变形模式,同时指出与所有假设应力模式正交的非零变形为零能机动模式,从而可以用简单变形模式方便地识别和抑制单元零能模式。在此基础上利用初始应力模式与简单变形模式的正交性提出一种假设杂交元应力场的有效方法,结合等函数法应力模式组成初始应力模式,不仅可以根据实际问题需要灵活地假设不同分布规律的应力场,而且所形成杂交元完全避免零能机动模式。在数值算例中采用本文方法分别形成了2D-4节点杂交元和3D-8节点杂交元的多种假设应力场,表明本文所提出方法是有效可行的。 相似文献
70.