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分子印迹技术是一种制备具有分子识别能力的聚合物的有效技术,已经广泛应用于制备对小分子具有选择性的分子印迹聚合物,但制备能够特异性识别生物大分子--蛋白质的分子印迹聚合物的研究仍然具有挑战性。本文讨论了制备蛋白质分子印迹聚合物的难点,评述了目前印迹蛋白质的方法及各自的优缺点,展望了蛋白质印迹技术的发展趋势。 相似文献
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水相识别分子印迹技术 总被引:1,自引:0,他引:1
在各种基于超分子方法的仿生识别体系中,分子印迹聚合物已经证明是一种有潜力的合成受体,受到了广泛的关注。传统的分子印迹技术通常是在有机溶剂中制备对小分子具有选择性的印迹聚合物,而在水相中制备及识别生物大分子的研究仍具有相当的挑战性。从小分子到生物大分子、从有机相到水相,反映了分子印迹技术的发展趋势。本文对最近几年分子印迹在水相制备与识别方面的最新进展进行了总结与评述,探讨了水相识别印迹聚合物的设计策略与制备方法;着重介绍了水相识别技术在固相萃取、色谱固定相、药物控释、中药有效成份提取以及生物分子识别等方面的应用;指出了提高水相识别选择性的途径并对其将来的发展进行了建议与展望。 相似文献
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分子印迹聚合物研究:从小分子到生物大分子 总被引:4,自引:0,他引:4
分子印迹技术是一项制备功能聚合物材料的方法,其对印迹分子的专一性选择识别能力引起了人们的广泛关注。随着方法的基本确立和技术的逐渐成熟,其应用领域和范围不断扩大。本文在总结以往研究结果的基础上,对迄今为止进展相对缓慢的生物大分子印迹研究予以了特别关注,对相关的水环境下的分子识别问题进行了仔细的讨论,认真的分析了生物大分子印迹研究工作的难点和不利因素,对分子印迹技术的未来发展和应用前景进行了展望。 相似文献
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分子印迹技术是将分子设计、大分子合成、分子识别、生物模拟以及生物工程等技术的优点结合起来发展而成的一种新兴跨学科方法,所形成的分子印迹聚合物亲合性好、选择性和稳定性高。二氧化钛及其复合材料价格低廉、安全高效、绿色环保、光催化活性高,被广泛应用于光催化、光电转换等领域。二氧化钛及其复合材料形成的分子印迹聚合物稳定性高,光催化活性好,并且具有良好的选择性,能对低浓度、高毒性污染物进行选择性富集及光催化降解,拓宽了其应用范围。本文综述了近二十年来分子印迹型二氧化钛及其复合材料的制备方法及其在光催化降解、传感器构建以及其他领域的应用概况,并进行展望。 相似文献
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氟哌酸分子印迹共混膜的制备及结构表征 总被引:2,自引:0,他引:2
以氟哌酸为模板分子,α-甲基丙烯酸为功能单体,三甲氧基丙烷三甲基丙烯酸酯为交联剂,利用本体聚合方法制备了具有特异选择性的氟哌酸分子印迹聚合物,并利用聚砜与氟哌酸分子印迹聚合物共混的方法制备了分子印迹聚合物膜.运用红外光谱分析和透射电子显微镜研究了氟哌酸分子印迹聚合物的结构.运用扫描电子显微镜研究了分子识别膜的机理和吸附性能,运用平衡结合实验法证明了分子识别膜对氟哌酸表现出了较高的选择性. 相似文献
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Lu Y Chen X Hu W Lu N Sun J Shen J 《Langmuir : the ACS journal of surfaces and colloids》2007,23(6):3254-3259
Layer-by-layer assembled polyelectrolyte multilayer films of poly(acrylic acid) (PAA)/poly(allylamine hydrochloride) (PAH) have been successfully patterned by room-temperature imprinting using a Norland Optical Adhesives (NOA 63) polymer mold. The proper amount of water in the PAA/PAH multilayer film can decrease the viscosity of the film and facilitate the imprinting. Many factors, such as imprinting pressure, length of imprinting time, and the structure and size of the patterns in the polymer mold, can produce an influence on the final imprinted pattern structures on multilayer films. A high imprinting pressure of 100 bar and elongated imprinting time of several hours is needed to achieve a patterned PAA/PAH multilayer film with a feature size of several tens of micrometers. With a twice imprinting, grid structures can be successfully produced when a NOA 63 mold having line structures is used. Room-temperature imprinting by using polymer NOA 63 mold provides a facile way to fabricate layered polymeric films with various kinds of pattern structures. 相似文献
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为了在含水介质中进行有效印迹,本研究中以双甲基丙烯酰-β-环糊精(BMA-β-CD)和2-(二乙基胺基)乙基甲基丙烯酸酯(DEAEM)为功能单体制备了胆酸印迹聚合物MIP1,并用平衡结合实验研究了MIP1在含水介质中对模板分子的识别能力。结果表明,MIP1比单独以BMA-β-CD或DEAEM为功能单体制备的印迹聚合物MIP2和MIP3,显示出对模板分子更好的选择性结合能力。MIP1的特异性吸附量ΔCP为38.81μmol/g,印迹因子IF为2.46。研究表明,在含水介质中,利用模板分子与功能单体之间的疏水作用和离子作用是提高印迹聚合物分子识别能力的关键。研究还表明,在识别过程中,疏水作用在驱动分子进入印迹孔穴时起重要作用。 相似文献
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Shi-Hui Song Kazumi Shirasaka Yasuhito Hirokawa Hiroyuki Asanuma Takehiko Wada Jun Sumaoka 《Supramolecular chemistry》2013,25(3):149-155
β-Cyclodextrin (β-CyD)-based polymeric receptors for γ-endorphin (γ-endor, an opioid heptadecapeptide) were prepared using the molecular imprinting method. When mono-3-(N-acrylamido)-3-deoxy-β-CyD bearing a vinyl group in the secondary hydroxyl side of the cavity of β-CyD was polymerised in water in the presence of γ-endor, the binding activity of the β-CyD polymer to this peptide in water was enormously promoted by the imprinting. By contrast, the bindings towards methionine–enkephalin (N-terminal pentapeptide of γ-endor) and its homologue leucine–enkephalin were suppressed. Thus, the binding of γ-endor by the imprinted polymer was highly selective. The imprinting towards γ-endor was also successful with the use of the β-CyD monomer bearing a vinyl group in the primary hydroxyl side of the cavity, although the recognition was less strict. Various factors affecting the imprinting efficiency (kinds of β-CyD vinyl monomer and template, as well as the pH of imprinting mixture) are discussed. 相似文献
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Molecularly imprinted polymers using serotonin as the template molecule was prepared for selective recognition from platelet rich plasma by non-covalent imprinting approach. Four different monomers (methacrylic acid, acrylamide, 4-vinylpyridine and 2-acrylamido-2-methylpropane sulfonic acid) and acetonitrile and DMSO as porogen were investigated for the first time by bulk polymerization. The molecularly imprinted polymer which was prepared by acrylamide/methacrylic acid had the largest imprinting factor for serotonin. The affinity and specificity of these polymers were evaluated by equilibrium binding experiments. The effect of polarity of the solvents was examined by polymers binding capacity and imprinting factor. According to the Scatchard analysis the K(d) and Q(max) values were calculated as 1.95 micromoll(-1) and 19.129 micromolg(-1), respectively. The polymer was tested to evaluate serotonin from platelet rich plasma and 70% serotonin recovery was found. 相似文献
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Currently, small proteins imprinting are more reported since large proteins molecular imprinting faces challenge due to their bulk size and complex structure. In this work, bovine serum albumin (BSA) surface-imprinted magnetic polymer was successfully synthesized based on atomic transfer radical polymerization (ATRP) method in the presence of common monomer (N-isopropylacrylamide) with the assistant of basic functional monomer (N-[3-(dimethylamino)propyl]-methacrylamide), which provides a achievable attempt for imprinting larger target proteins based on the ATPR with the mild reaction conditions. The BSA-imprinted polymer exhibited higher adsorption capacity and selectivity to BSA over the non-imprinted polymer. Competitive adsorption tests indicated the BSA-imprinted polymer had better selective adsorption and recognition properties to BSA in the mixture. The obtained BSA-imprinted polymer was applied to bovine serum, which also showed selectivity to BSA. In addition, a conventional aqueous two-phase solution of PEG/sulphate was used as elution for adsorbed BSA, which was compared with common NaCl elution. 相似文献
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Mechanism of molecular recognition on molecular imprinted monolith by capillary electrochromatography 总被引:2,自引:0,他引:2
The recognition mechanism of molecularly imprinted polymer (MIP) in capillary electrochromatography (CEC) is complicated since it possesses a hybrid process, which comprises the features of chromatographic retention, electrophoretic migration and molecular imprinting. For an understanding of the molecular recognition of MIP in CEC, a monolithic MIP in a capillary with 1,1'-binaphthyl-2,2'-diamine (BNA) imprinting was prepared by in situ copolymerization of imprinted molecule, methacrylic acid and ethylene glycol dimethacrylate in porogenic solvent, a mixture of toluene-isooctane. Strong recognition ability and high column performance (theory plates was 43,000 plates/m) of BNA were achieved on this monolithic MIP in CEC mode. In addition, BNA and its structural analogue, 1,1'-bi-2, 2'-naphthol, differing in functional groups, were used as model compounds to study imprinting effect on the resultant BNA-imprinted monolithic column, a reference column without imprinting of BNA and a open capillary. The effects of organic modifier concentration, pH value of buffer, salt concentration of buffer and column temperature on the retention and recognition of two compounds were investigated. The results showed that the molecular recognition on MIP monolith in CEC mode mainly derived from imprinting cavities on BNA-imprinted polymer other than chromatographic retention and electrophoretic migration. 相似文献